Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18095 and CA265_RS18100 are separated by 77 nucleotides CA265_RS18100 and CA265_RS18105 are separated by 2 nucleotides CA265_RS18105 and CA265_RS18110 are separated by 32 nucleotides CA265_RS18110 and CA265_RS18115 are separated by 108 nucleotides
CA265_RS18095: CA265_RS18095 - peptidase, at 4,517,308 to 4,519,527
_RS18095
CA265_RS18100: CA265_RS18100 - hypothetical protein, at 4,519,605 to 4,519,835
_RS18100
CA265_RS18105: CA265_RS18105 - hypothetical protein, at 4,519,838 to 4,520,206
_RS18105
CA265_RS18110: CA265_RS18110 - excinuclease ABC subunit C, at 4,520,239 to 4,522,041
_RS18110
CA265_RS18115: CA265_RS18115 - gliding motility protein GldN, at 4,522,150 to 4,523,064
_RS18115
Position (kb)
4520
4521
4522
4523 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4519.459 kb on + strand at 4519.525 kb on + strand at 4519.530 kb on - strand at 4519.530 kb on - strand at 4519.534 kb on + strand at 4519.802 kb on + strand, within CA265_RS18100 at 4519.804 kb on + strand, within CA265_RS18100 at 4519.812 kb on - strand, within CA265_RS18100 at 4519.856 kb on - strand at 4519.858 kb on + strand at 4519.866 kb on + strand at 4519.883 kb on - strand, within CA265_RS18105 at 4519.915 kb on - strand, within CA265_RS18105 at 4519.945 kb on + strand, within CA265_RS18105 at 4519.978 kb on + strand, within CA265_RS18105 at 4519.979 kb on - strand, within CA265_RS18105 at 4519.979 kb on - strand, within CA265_RS18105 at 4519.979 kb on - strand, within CA265_RS18105 at 4520.016 kb on - strand, within CA265_RS18105 at 4520.024 kb on - strand, within CA265_RS18105 at 4520.042 kb on + strand, within CA265_RS18105 at 4520.043 kb on - strand, within CA265_RS18105 at 4520.043 kb on - strand, within CA265_RS18105 at 4520.044 kb on + strand, within CA265_RS18105 at 4520.044 kb on + strand, within CA265_RS18105 at 4520.044 kb on + strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.047 kb on - strand, within CA265_RS18105 at 4520.047 kb on - strand, within CA265_RS18105 at 4520.047 kb on - strand, within CA265_RS18105 at 4520.047 kb on - strand, within CA265_RS18105 at 4520.086 kb on - strand, within CA265_RS18105 at 4520.156 kb on - strand, within CA265_RS18105 at 4520.160 kb on - strand, within CA265_RS18105 at 4520.194 kb on + strand at 4520.201 kb on + strand at 4520.230 kb on + strand at 4520.283 kb on + strand at 4520.283 kb on + strand at 4520.297 kb on - strand at 4520.329 kb on + strand at 4520.344 kb on - strand at 4520.372 kb on - strand at 4520.415 kb on - strand at 4520.434 kb on - strand, within CA265_RS18110 at 4520.619 kb on + strand, within CA265_RS18110 at 4520.619 kb on + strand, within CA265_RS18110 at 4520.620 kb on - strand, within CA265_RS18110 at 4520.620 kb on - strand, within CA265_RS18110 at 4520.658 kb on + strand, within CA265_RS18110 at 4520.658 kb on + strand, within CA265_RS18110 at 4520.659 kb on - strand, within CA265_RS18110 at 4520.659 kb on - strand, within CA265_RS18110 at 4520.687 kb on - strand, within CA265_RS18110 at 4520.762 kb on - strand, within CA265_RS18110 at 4520.778 kb on + strand, within CA265_RS18110 at 4520.779 kb on - strand, within CA265_RS18110 at 4520.828 kb on + strand, within CA265_RS18110 at 4520.938 kb on + strand, within CA265_RS18110 at 4520.938 kb on + strand, within CA265_RS18110 at 4520.951 kb on + strand, within CA265_RS18110 at 4520.952 kb on - strand, within CA265_RS18110 at 4520.968 kb on + strand, within CA265_RS18110 at 4520.969 kb on - strand, within CA265_RS18110 at 4520.991 kb on - strand, within CA265_RS18110 at 4521.011 kb on + strand, within CA265_RS18110 at 4521.011 kb on + strand, within CA265_RS18110 at 4521.011 kb on + strand, within CA265_RS18110 at 4521.011 kb on + strand, within CA265_RS18110 at 4521.059 kb on - strand, within CA265_RS18110 at 4521.108 kb on + strand, within CA265_RS18110 at 4521.222 kb on - strand, within CA265_RS18110 at 4521.299 kb on + strand, within CA265_RS18110 at 4521.331 kb on + strand, within CA265_RS18110 at 4521.331 kb on + strand, within CA265_RS18110 at 4521.419 kb on + strand, within CA265_RS18110 at 4521.419 kb on + strand, within CA265_RS18110 at 4521.419 kb on + strand, within CA265_RS18110 at 4521.420 kb on - strand, within CA265_RS18110 at 4521.481 kb on - strand, within CA265_RS18110 at 4521.544 kb on - strand, within CA265_RS18110 at 4521.558 kb on + strand, within CA265_RS18110 at 4521.583 kb on + strand, within CA265_RS18110 at 4521.681 kb on - strand, within CA265_RS18110 at 4521.742 kb on + strand, within CA265_RS18110 at 4521.766 kb on + strand, within CA265_RS18110 at 4521.767 kb on - strand, within CA265_RS18110 at 4521.837 kb on + strand, within CA265_RS18110 at 4522.042 kb on - strand at 4522.048 kb on + strand at 4522.090 kb on - strand at 4522.090 kb on - strand at 4522.106 kb on - strand at 4522.144 kb on + strand at 4522.148 kb on - strand at 4522.152 kb on - strand at 4522.155 kb on - strand at 4522.211 kb on + strand at 4522.236 kb on + strand at 4522.237 kb on - strand at 4522.240 kb on - strand at 4522.277 kb on - strand, within CA265_RS18115 at 4522.284 kb on - strand, within CA265_RS18115 at 4522.284 kb on - strand, within CA265_RS18115 at 4522.294 kb on + strand, within CA265_RS18115 at 4522.392 kb on - strand, within CA265_RS18115 at 4522.394 kb on + strand, within CA265_RS18115 at 4522.451 kb on - strand, within CA265_RS18115 at 4522.451 kb on - strand, within CA265_RS18115 at 4522.451 kb on - strand, within CA265_RS18115 at 4522.452 kb on + strand, within CA265_RS18115 at 4522.452 kb on + strand, within CA265_RS18115 at 4522.452 kb on + strand, within CA265_RS18115 at 4522.453 kb on - strand, within CA265_RS18115 at 4522.457 kb on - strand, within CA265_RS18115 at 4522.457 kb on - strand, within CA265_RS18115 at 4522.461 kb on + strand, within CA265_RS18115 at 4522.462 kb on - strand, within CA265_RS18115 at 4522.528 kb on + strand, within CA265_RS18115 at 4522.548 kb on + strand, within CA265_RS18115 at 4522.553 kb on - strand, within CA265_RS18115 at 4522.683 kb on - strand, within CA265_RS18115 at 4522.683 kb on - strand, within CA265_RS18115 at 4522.718 kb on - strand, within CA265_RS18115 at 4522.720 kb on + strand, within CA265_RS18115 at 4522.721 kb on - strand, within CA265_RS18115 at 4522.761 kb on + strand, within CA265_RS18115 at 4522.775 kb on - strand, within CA265_RS18115 at 4522.888 kb on - strand, within CA265_RS18115 at 4522.899 kb on + strand, within CA265_RS18115 at 4522.920 kb on + strand, within CA265_RS18115 at 4522.964 kb on - strand, within CA265_RS18115 at 4522.995 kb on + strand at 4523.027 kb on - strand at 4523.040 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 4,519,459 + -1.6 4,519,525 + +0.3 4,519,530 - -1.9 4,519,530 - -1.0 4,519,534 + -0.4 4,519,802 + CA265_RS18100 0.85 -0.1 4,519,804 + CA265_RS18100 0.86 -0.3 4,519,812 - CA265_RS18100 0.90 +0.1 4,519,856 - +0.1 4,519,858 + -0.4 4,519,866 + +0.5 4,519,883 - CA265_RS18105 0.12 +0.0 4,519,915 - CA265_RS18105 0.21 -0.3 4,519,945 + CA265_RS18105 0.29 -0.2 4,519,978 + CA265_RS18105 0.38 -0.9 4,519,979 - CA265_RS18105 0.38 -0.5 4,519,979 - CA265_RS18105 0.38 -0.5 4,519,979 - CA265_RS18105 0.38 -1.2 4,520,016 - CA265_RS18105 0.48 -0.3 4,520,024 - CA265_RS18105 0.50 -0.3 4,520,042 + CA265_RS18105 0.55 +0.2 4,520,043 - CA265_RS18105 0.56 +0.6 4,520,043 - CA265_RS18105 0.56 +0.6 4,520,044 + CA265_RS18105 0.56 -0.1 4,520,044 + CA265_RS18105 0.56 +0.3 4,520,044 + CA265_RS18105 0.56 +0.5 4,520,045 - CA265_RS18105 0.56 -0.1 4,520,045 - CA265_RS18105 0.56 +0.1 4,520,045 - CA265_RS18105 0.56 -2.0 4,520,045 - CA265_RS18105 0.56 -1.9 4,520,045 - CA265_RS18105 0.56 -0.6 4,520,047 - CA265_RS18105 0.57 -0.5 4,520,047 - CA265_RS18105 0.57 -0.8 4,520,047 - CA265_RS18105 0.57 +0.1 4,520,047 - CA265_RS18105 0.57 -0.4 4,520,086 - CA265_RS18105 0.67 +2.8 4,520,156 - CA265_RS18105 0.86 -0.2 4,520,160 - CA265_RS18105 0.87 -0.9 4,520,194 + +1.0 4,520,201 + -0.6 4,520,230 + +0.5 4,520,283 + +0.6 4,520,283 + -0.7 4,520,297 - -0.4 4,520,329 + -1.2 4,520,344 - -2.4 4,520,372 - +0.3 4,520,415 - -0.8 4,520,434 - CA265_RS18110 0.11 -2.4 4,520,619 + CA265_RS18110 0.21 -0.8 4,520,619 + CA265_RS18110 0.21 -0.0 4,520,620 - CA265_RS18110 0.21 +1.5 4,520,620 - CA265_RS18110 0.21 -0.5 4,520,658 + CA265_RS18110 0.23 -1.2 4,520,658 + CA265_RS18110 0.23 -1.1 4,520,659 - CA265_RS18110 0.23 +1.5 4,520,659 - CA265_RS18110 0.23 -0.8 4,520,687 - CA265_RS18110 0.25 -0.5 4,520,762 - CA265_RS18110 0.29 -1.2 4,520,778 + CA265_RS18110 0.30 -0.2 4,520,779 - CA265_RS18110 0.30 -1.4 4,520,828 + CA265_RS18110 0.33 -2.4 4,520,938 + CA265_RS18110 0.39 -0.6 4,520,938 + CA265_RS18110 0.39 -0.9 4,520,951 + CA265_RS18110 0.39 -1.4 4,520,952 - CA265_RS18110 0.40 -0.9 4,520,968 + CA265_RS18110 0.40 -0.8 4,520,969 - CA265_RS18110 0.40 -1.7 4,520,991 - CA265_RS18110 0.42 -1.9 4,521,011 + CA265_RS18110 0.43 -0.4 4,521,011 + CA265_RS18110 0.43 -0.3 4,521,011 + CA265_RS18110 0.43 -0.2 4,521,011 + CA265_RS18110 0.43 +0.2 4,521,059 - CA265_RS18110 0.45 -0.8 4,521,108 + CA265_RS18110 0.48 -0.3 4,521,222 - CA265_RS18110 0.55 -0.5 4,521,299 + CA265_RS18110 0.59 -0.4 4,521,331 + CA265_RS18110 0.61 -0.7 4,521,331 + CA265_RS18110 0.61 +0.3 4,521,419 + CA265_RS18110 0.65 -0.6 4,521,419 + CA265_RS18110 0.65 -0.3 4,521,419 + CA265_RS18110 0.65 +0.0 4,521,420 - CA265_RS18110 0.66 -1.3 4,521,481 - CA265_RS18110 0.69 -0.3 4,521,544 - CA265_RS18110 0.72 -0.9 4,521,558 + CA265_RS18110 0.73 -0.3 4,521,583 + CA265_RS18110 0.75 +0.5 4,521,681 - CA265_RS18110 0.80 -0.4 4,521,742 + CA265_RS18110 0.83 -1.3 4,521,766 + CA265_RS18110 0.85 -1.0 4,521,767 - CA265_RS18110 0.85 -0.7 4,521,837 + CA265_RS18110 0.89 -0.1 4,522,042 - +0.2 4,522,048 + +0.6 4,522,090 - -0.1 4,522,090 - +0.1 4,522,106 - +1.1 4,522,144 + -0.2 4,522,148 - -1.1 4,522,152 - +0.6 4,522,155 - -0.5 4,522,211 + -0.2 4,522,236 + -0.3 4,522,237 - -0.9 4,522,240 - -0.4 4,522,277 - CA265_RS18115 0.14 -0.3 4,522,284 - CA265_RS18115 0.15 +0.2 4,522,284 - CA265_RS18115 0.15 +2.2 4,522,294 + CA265_RS18115 0.16 +0.2 4,522,392 - CA265_RS18115 0.26 -0.2 4,522,394 + CA265_RS18115 0.27 +0.5 4,522,451 - CA265_RS18115 0.33 +0.2 4,522,451 - CA265_RS18115 0.33 -1.1 4,522,451 - CA265_RS18115 0.33 -0.5 4,522,452 + CA265_RS18115 0.33 +0.5 4,522,452 + CA265_RS18115 0.33 +0.9 4,522,452 + CA265_RS18115 0.33 +0.0 4,522,453 - CA265_RS18115 0.33 -0.5 4,522,457 - CA265_RS18115 0.34 -0.1 4,522,457 - CA265_RS18115 0.34 -0.4 4,522,461 + CA265_RS18115 0.34 +0.4 4,522,462 - CA265_RS18115 0.34 -0.1 4,522,528 + CA265_RS18115 0.41 +0.4 4,522,548 + CA265_RS18115 0.43 -0.9 4,522,553 - CA265_RS18115 0.44 +0.0 4,522,683 - CA265_RS18115 0.58 -0.1 4,522,683 - CA265_RS18115 0.58 +0.3 4,522,718 - CA265_RS18115 0.62 -0.3 4,522,720 + CA265_RS18115 0.62 -1.5 4,522,721 - CA265_RS18115 0.62 +0.5 4,522,761 + CA265_RS18115 0.67 -0.4 4,522,775 - CA265_RS18115 0.68 -1.4 4,522,888 - CA265_RS18115 0.81 -0.5 4,522,899 + CA265_RS18115 0.82 -0.7 4,522,920 + CA265_RS18115 0.84 +0.0 4,522,964 - CA265_RS18115 0.89 -0.3 4,522,995 + -0.2 4,523,027 - +0.5 4,523,040 + +0.4
Or see this region's nucleotide sequence