Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS16130 and CA265_RS16135 overlap by 1 nucleotides CA265_RS16135 and CA265_RS16140 are separated by 39 nucleotides CA265_RS16140 and CA265_RS16145 overlap by 1 nucleotides CA265_RS16145 and CA265_RS16150 are separated by 216 nucleotides CA265_RS16150 and CA265_RS16155 are separated by 234 nucleotides CA265_RS16155 and CA265_RS16160 overlap by 4 nucleotides
CA265_RS16130: CA265_RS16130 - cell division protein FtsX, at 4,054,232 to 4,055,110
_RS16130
CA265_RS16135: CA265_RS16135 - hypothetical protein, at 4,055,110 to 4,055,337
_RS16135
CA265_RS16140: CA265_RS16140 - hypothetical protein, at 4,055,377 to 4,055,580
_RS16140
CA265_RS16145: CA265_RS16145 - PIN domain nuclease, at 4,055,580 to 4,055,768
_RS16145
CA265_RS16150: CA265_RS16150 - UDP-diphosphatase, at 4,055,985 to 4,056,779
_RS16150
CA265_RS16155: CA265_RS16155 - tRNA pseudouridine(55) synthase TruB, at 4,057,014 to 4,057,745
_RS16155
CA265_RS16160: CA265_RS16160 - hypothetical protein, at 4,057,742 to 4,058,602
_RS16160
Position (kb)
4055
4056
4057 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4055.013 kb on + strand, within CA265_RS16130 at 4055.079 kb on + strand at 4055.348 kb on + strand at 4055.348 kb on + strand at 4055.353 kb on - strand at 4055.353 kb on - strand at 4055.373 kb on + strand at 4055.556 kb on - strand, within CA265_RS16140 at 4055.586 kb on + strand at 4055.587 kb on - strand at 4055.587 kb on - strand at 4055.590 kb on + strand at 4055.597 kb on + strand at 4055.597 kb on + strand at 4055.597 kb on + strand at 4055.597 kb on + strand at 4055.603 kb on + strand, within CA265_RS16145 at 4055.662 kb on + strand, within CA265_RS16145 at 4055.689 kb on + strand, within CA265_RS16145 at 4055.807 kb on + strand at 4055.807 kb on + strand at 4055.808 kb on - strand at 4055.825 kb on + strand at 4055.834 kb on - strand at 4055.854 kb on - strand at 4055.856 kb on + strand at 4055.907 kb on - strand at 4055.925 kb on + strand at 4055.984 kb on + strand at 4056.014 kb on + strand at 4056.015 kb on - strand at 4056.046 kb on + strand at 4056.116 kb on - strand, within CA265_RS16150 at 4056.146 kb on - strand, within CA265_RS16150 at 4056.149 kb on - strand, within CA265_RS16150 at 4056.160 kb on + strand, within CA265_RS16150 at 4056.204 kb on - strand, within CA265_RS16150 at 4056.209 kb on - strand, within CA265_RS16150 at 4056.212 kb on - strand, within CA265_RS16150 at 4056.250 kb on + strand, within CA265_RS16150 at 4056.307 kb on + strand, within CA265_RS16150 at 4056.385 kb on - strand, within CA265_RS16150 at 4056.388 kb on + strand, within CA265_RS16150 at 4056.420 kb on - strand, within CA265_RS16150 at 4056.458 kb on + strand, within CA265_RS16150 at 4056.459 kb on - strand, within CA265_RS16150 at 4056.526 kb on + strand, within CA265_RS16150 at 4056.526 kb on + strand, within CA265_RS16150 at 4056.534 kb on - strand, within CA265_RS16150 at 4056.534 kb on - strand, within CA265_RS16150 at 4056.638 kb on + strand, within CA265_RS16150 at 4056.640 kb on + strand, within CA265_RS16150 at 4056.696 kb on - strand, within CA265_RS16150 at 4056.773 kb on + strand at 4056.791 kb on - strand at 4056.791 kb on - strand at 4056.791 kb on - strand at 4056.823 kb on - strand at 4056.826 kb on + strand at 4056.892 kb on - strand at 4057.076 kb on + strand at 4057.076 kb on + strand at 4057.171 kb on + strand, within CA265_RS16155 at 4057.172 kb on - strand, within CA265_RS16155 at 4057.172 kb on - strand, within CA265_RS16155 at 4057.172 kb on - strand, within CA265_RS16155 at 4057.199 kb on + strand, within CA265_RS16155 at 4057.199 kb on + strand, within CA265_RS16155 at 4057.216 kb on + strand, within CA265_RS16155 at 4057.229 kb on - strand, within CA265_RS16155 at 4057.259 kb on + strand, within CA265_RS16155 at 4057.340 kb on + strand, within CA265_RS16155 at 4057.390 kb on + strand, within CA265_RS16155 at 4057.391 kb on - strand, within CA265_RS16155 at 4057.391 kb on - strand, within CA265_RS16155 at 4057.448 kb on - strand, within CA265_RS16155 at 4057.570 kb on - strand, within CA265_RS16155 at 4057.570 kb on - strand, within CA265_RS16155 at 4057.684 kb on + strand at 4057.684 kb on + strand at 4057.694 kb on + strand at 4057.774 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 4,055,013 + CA265_RS16130 0.89 +0.6 4,055,079 + +0.4 4,055,348 + -0.3 4,055,348 + -0.6 4,055,353 - -1.0 4,055,353 - -2.0 4,055,373 + -0.1 4,055,556 - CA265_RS16140 0.88 -0.1 4,055,586 + -1.2 4,055,587 - -1.1 4,055,587 - -2.1 4,055,590 + -0.7 4,055,597 + -1.1 4,055,597 + +0.4 4,055,597 + -0.5 4,055,597 + +0.5 4,055,603 + CA265_RS16145 0.12 -0.7 4,055,662 + CA265_RS16145 0.43 -0.9 4,055,689 + CA265_RS16145 0.58 -0.1 4,055,807 + +2.4 4,055,807 + +0.7 4,055,808 - -1.3 4,055,825 + -0.6 4,055,834 - -0.6 4,055,854 - -0.1 4,055,856 + -0.9 4,055,907 - -1.2 4,055,925 + -0.1 4,055,984 + +0.3 4,056,014 + -1.6 4,056,015 - -1.4 4,056,046 + -1.8 4,056,116 - CA265_RS16150 0.16 -0.9 4,056,146 - CA265_RS16150 0.20 -1.3 4,056,149 - CA265_RS16150 0.21 +1.2 4,056,160 + CA265_RS16150 0.22 -0.5 4,056,204 - CA265_RS16150 0.28 -1.0 4,056,209 - CA265_RS16150 0.28 +0.0 4,056,212 - CA265_RS16150 0.29 -0.8 4,056,250 + CA265_RS16150 0.33 -0.6 4,056,307 + CA265_RS16150 0.41 -0.9 4,056,385 - CA265_RS16150 0.50 -1.8 4,056,388 + CA265_RS16150 0.51 -0.6 4,056,420 - CA265_RS16150 0.55 -1.3 4,056,458 + CA265_RS16150 0.59 +1.0 4,056,459 - CA265_RS16150 0.60 -0.2 4,056,526 + CA265_RS16150 0.68 +0.4 4,056,526 + CA265_RS16150 0.68 -0.4 4,056,534 - CA265_RS16150 0.69 -0.7 4,056,534 - CA265_RS16150 0.69 -1.9 4,056,638 + CA265_RS16150 0.82 -0.7 4,056,640 + CA265_RS16150 0.82 -0.6 4,056,696 - CA265_RS16150 0.89 -1.4 4,056,773 + -1.2 4,056,791 - -0.8 4,056,791 - +0.4 4,056,791 - -0.9 4,056,823 - -0.0 4,056,826 + -0.4 4,056,892 - +0.4 4,057,076 + +0.8 4,057,076 + +0.0 4,057,171 + CA265_RS16155 0.21 +1.4 4,057,172 - CA265_RS16155 0.22 +0.4 4,057,172 - CA265_RS16155 0.22 +0.3 4,057,172 - CA265_RS16155 0.22 +0.8 4,057,199 + CA265_RS16155 0.25 -0.6 4,057,199 + CA265_RS16155 0.25 -2.4 4,057,216 + CA265_RS16155 0.28 -0.3 4,057,229 - CA265_RS16155 0.29 +2.5 4,057,259 + CA265_RS16155 0.33 +0.0 4,057,340 + CA265_RS16155 0.45 +1.4 4,057,390 + CA265_RS16155 0.51 +0.1 4,057,391 - CA265_RS16155 0.52 +1.6 4,057,391 - CA265_RS16155 0.52 -0.8 4,057,448 - CA265_RS16155 0.59 +1.1 4,057,570 - CA265_RS16155 0.76 +0.7 4,057,570 - CA265_RS16155 0.76 +0.4 4,057,684 + +0.2 4,057,684 + -1.1 4,057,694 + -1.3 4,057,774 + +0.5
Or see this region's nucleotide sequence