Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08385 and CA265_RS08390 are separated by 53 nucleotides CA265_RS08390 and CA265_RS08395 are separated by 173 nucleotides
CA265_RS08385: CA265_RS08385 - sodium:solute symporter, at 2,082,575 to 2,084,143
_RS08385
CA265_RS08390: CA265_RS08390 - dihydrodipicolinate synthase family protein, at 2,084,197 to 2,085,129
_RS08390
CA265_RS08395: CA265_RS08395 - AraC family transcriptional regulator, at 2,085,303 to 2,086,172
_RS08395
Position (kb)
2084
2085
2086 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2083.246 kb on + strand, within CA265_RS08385 at 2083.246 kb on + strand, within CA265_RS08385 at 2083.366 kb on + strand, within CA265_RS08385 at 2083.406 kb on + strand, within CA265_RS08385 at 2083.407 kb on - strand, within CA265_RS08385 at 2083.409 kb on - strand, within CA265_RS08385 at 2083.451 kb on + strand, within CA265_RS08385 at 2083.452 kb on - strand, within CA265_RS08385 at 2083.452 kb on - strand, within CA265_RS08385 at 2083.457 kb on + strand, within CA265_RS08385 at 2083.504 kb on - strand, within CA265_RS08385 at 2083.525 kb on - strand, within CA265_RS08385 at 2083.525 kb on - strand, within CA265_RS08385 at 2083.642 kb on - strand, within CA265_RS08385 at 2083.730 kb on - strand, within CA265_RS08385 at 2083.771 kb on - strand, within CA265_RS08385 at 2083.785 kb on + strand, within CA265_RS08385 at 2083.987 kb on - strand at 2083.987 kb on - strand at 2084.169 kb on - strand at 2084.193 kb on + strand at 2084.236 kb on - strand at 2084.278 kb on - strand at 2084.300 kb on + strand, within CA265_RS08390 at 2084.322 kb on - strand, within CA265_RS08390 at 2084.457 kb on + strand, within CA265_RS08390 at 2084.458 kb on - strand, within CA265_RS08390 at 2084.484 kb on - strand, within CA265_RS08390 at 2084.528 kb on + strand, within CA265_RS08390 at 2084.643 kb on - strand, within CA265_RS08390 at 2084.676 kb on + strand, within CA265_RS08390 at 2084.728 kb on - strand, within CA265_RS08390 at 2084.780 kb on - strand, within CA265_RS08390 at 2084.786 kb on + strand, within CA265_RS08390 at 2084.846 kb on - strand, within CA265_RS08390 at 2084.919 kb on - strand, within CA265_RS08390 at 2084.919 kb on - strand, within CA265_RS08390 at 2084.919 kb on - strand, within CA265_RS08390 at 2084.941 kb on - strand, within CA265_RS08390 at 2085.004 kb on - strand, within CA265_RS08390 at 2085.006 kb on + strand, within CA265_RS08390 at 2085.033 kb on - strand, within CA265_RS08390 at 2085.057 kb on + strand at 2085.057 kb on + strand at 2085.108 kb on - strand at 2085.111 kb on + strand at 2085.119 kb on + strand at 2085.126 kb on - strand at 2085.127 kb on + strand at 2085.135 kb on + strand at 2085.170 kb on + strand at 2085.273 kb on - strand at 2085.290 kb on - strand at 2085.347 kb on + strand at 2085.375 kb on - strand at 2085.378 kb on + strand at 2085.387 kb on - strand at 2085.571 kb on + strand, within CA265_RS08395 at 2085.581 kb on - strand, within CA265_RS08395 at 2085.596 kb on - strand, within CA265_RS08395 at 2085.644 kb on - strand, within CA265_RS08395 at 2085.664 kb on - strand, within CA265_RS08395 at 2085.720 kb on - strand, within CA265_RS08395 at 2085.740 kb on + strand, within CA265_RS08395 at 2085.741 kb on - strand, within CA265_RS08395 at 2085.763 kb on - strand, within CA265_RS08395 at 2085.776 kb on + strand, within CA265_RS08395 at 2085.782 kb on + strand, within CA265_RS08395 at 2085.798 kb on + strand, within CA265_RS08395 at 2085.798 kb on + strand, within CA265_RS08395 at 2085.842 kb on - strand, within CA265_RS08395 at 2085.857 kb on + strand, within CA265_RS08395 at 2085.858 kb on - strand, within CA265_RS08395 at 2085.858 kb on - strand, within CA265_RS08395 at 2085.863 kb on + strand, within CA265_RS08395 at 2085.914 kb on + strand, within CA265_RS08395 at 2085.947 kb on + strand, within CA265_RS08395 at 2085.960 kb on - strand, within CA265_RS08395 at 2085.978 kb on + strand, within CA265_RS08395 at 2086.017 kb on - strand, within CA265_RS08395 at 2086.054 kb on + strand, within CA265_RS08395 at 2086.110 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 2,083,246 + CA265_RS08385 0.43 +1.7 2,083,246 + CA265_RS08385 0.43 +2.1 2,083,366 + CA265_RS08385 0.50 +1.1 2,083,406 + CA265_RS08385 0.53 +1.7 2,083,407 - CA265_RS08385 0.53 -0.1 2,083,409 - CA265_RS08385 0.53 -0.7 2,083,451 + CA265_RS08385 0.56 +0.7 2,083,452 - CA265_RS08385 0.56 -0.5 2,083,452 - CA265_RS08385 0.56 +0.1 2,083,457 + CA265_RS08385 0.56 +1.1 2,083,504 - CA265_RS08385 0.59 +0.1 2,083,525 - CA265_RS08385 0.61 -0.0 2,083,525 - CA265_RS08385 0.61 -0.3 2,083,642 - CA265_RS08385 0.68 +0.4 2,083,730 - CA265_RS08385 0.74 -1.2 2,083,771 - CA265_RS08385 0.76 +0.4 2,083,785 + CA265_RS08385 0.77 +0.5 2,083,987 - -0.4 2,083,987 - -0.0 2,084,169 - -0.4 2,084,193 + +0.5 2,084,236 - -0.2 2,084,278 - -0.7 2,084,300 + CA265_RS08390 0.11 +0.6 2,084,322 - CA265_RS08390 0.13 +0.6 2,084,457 + CA265_RS08390 0.28 +0.3 2,084,458 - CA265_RS08390 0.28 +0.7 2,084,484 - CA265_RS08390 0.31 -0.2 2,084,528 + CA265_RS08390 0.35 +0.5 2,084,643 - CA265_RS08390 0.48 -1.2 2,084,676 + CA265_RS08390 0.51 +0.1 2,084,728 - CA265_RS08390 0.57 +0.8 2,084,780 - CA265_RS08390 0.62 +0.5 2,084,786 + CA265_RS08390 0.63 -0.4 2,084,846 - CA265_RS08390 0.70 +0.4 2,084,919 - CA265_RS08390 0.77 +0.1 2,084,919 - CA265_RS08390 0.77 +1.1 2,084,919 - CA265_RS08390 0.77 +0.0 2,084,941 - CA265_RS08390 0.80 -2.2 2,085,004 - CA265_RS08390 0.86 +0.0 2,085,006 + CA265_RS08390 0.87 +0.3 2,085,033 - CA265_RS08390 0.90 -0.3 2,085,057 + -0.1 2,085,057 + +0.3 2,085,108 - -0.2 2,085,111 + -0.2 2,085,119 + +1.2 2,085,126 - -0.1 2,085,127 + +1.0 2,085,135 + +0.7 2,085,170 + +0.3 2,085,273 - -0.2 2,085,290 - +0.6 2,085,347 + -0.5 2,085,375 - +0.6 2,085,378 + -0.2 2,085,387 - -0.2 2,085,571 + CA265_RS08395 0.31 -1.6 2,085,581 - CA265_RS08395 0.32 +1.6 2,085,596 - CA265_RS08395 0.34 -0.5 2,085,644 - CA265_RS08395 0.39 +0.4 2,085,664 - CA265_RS08395 0.41 -2.2 2,085,720 - CA265_RS08395 0.48 -0.3 2,085,740 + CA265_RS08395 0.50 -0.4 2,085,741 - CA265_RS08395 0.50 -0.1 2,085,763 - CA265_RS08395 0.53 +0.7 2,085,776 + CA265_RS08395 0.54 +0.4 2,085,782 + CA265_RS08395 0.55 +0.1 2,085,798 + CA265_RS08395 0.57 -0.2 2,085,798 + CA265_RS08395 0.57 -1.0 2,085,842 - CA265_RS08395 0.62 +1.2 2,085,857 + CA265_RS08395 0.64 -0.6 2,085,858 - CA265_RS08395 0.64 +0.5 2,085,858 - CA265_RS08395 0.64 +1.0 2,085,863 + CA265_RS08395 0.64 +1.4 2,085,914 + CA265_RS08395 0.70 -0.1 2,085,947 + CA265_RS08395 0.74 +0.0 2,085,960 - CA265_RS08395 0.76 +0.5 2,085,978 + CA265_RS08395 0.78 +0.3 2,086,017 - CA265_RS08395 0.82 -0.5 2,086,054 + CA265_RS08395 0.86 +0.9 2,086,110 - +0.3
Or see this region's nucleotide sequence