Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05710 and CA265_RS05715 are separated by 40 nucleotides CA265_RS05715 and CA265_RS05720 are separated by 681 nucleotides
CA265_RS05710: CA265_RS05710 - hydroxyproline-2-epimerase, at 1,388,343 to 1,389,344
_RS05710
CA265_RS05715: CA265_RS05715 - amino acid dehydrogenase, at 1,389,385 to 1,390,626
_RS05715
CA265_RS05720: CA265_RS05720 - hypothetical protein, at 1,391,308 to 1,393,236
_RS05720
Position (kb)
1389
1390
1391 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 1388.634 kb on - strand, within CA265_RS05710 at 1388.634 kb on - strand, within CA265_RS05710 at 1388.643 kb on - strand, within CA265_RS05710 at 1388.644 kb on + strand, within CA265_RS05710 at 1388.645 kb on - strand, within CA265_RS05710 at 1388.775 kb on + strand, within CA265_RS05710 at 1388.801 kb on - strand, within CA265_RS05710 at 1388.836 kb on + strand, within CA265_RS05710 at 1388.836 kb on + strand, within CA265_RS05710 at 1388.901 kb on + strand, within CA265_RS05710 at 1388.921 kb on + strand, within CA265_RS05710 at 1388.962 kb on - strand, within CA265_RS05710 at 1388.963 kb on + strand, within CA265_RS05710 at 1388.963 kb on + strand, within CA265_RS05710 at 1388.964 kb on - strand, within CA265_RS05710 at 1388.964 kb on - strand, within CA265_RS05710 at 1388.980 kb on + strand, within CA265_RS05710 at 1389.003 kb on - strand, within CA265_RS05710 at 1389.010 kb on + strand, within CA265_RS05710 at 1389.010 kb on + strand, within CA265_RS05710 at 1389.011 kb on - strand, within CA265_RS05710 at 1389.011 kb on - strand, within CA265_RS05710 at 1389.038 kb on + strand, within CA265_RS05710 at 1389.066 kb on + strand, within CA265_RS05710 at 1389.133 kb on - strand, within CA265_RS05710 at 1389.182 kb on + strand, within CA265_RS05710 at 1389.248 kb on - strand at 1389.257 kb on - strand at 1389.257 kb on - strand at 1389.282 kb on + strand at 1389.299 kb on - strand at 1389.318 kb on + strand at 1389.372 kb on - strand at 1389.373 kb on + strand at 1389.403 kb on - strand at 1389.418 kb on + strand at 1389.419 kb on + strand at 1389.419 kb on + strand at 1389.437 kb on - strand at 1389.439 kb on + strand at 1389.440 kb on - strand at 1389.465 kb on - strand at 1389.487 kb on - strand at 1389.527 kb on + strand, within CA265_RS05715 at 1389.611 kb on - strand, within CA265_RS05715 at 1389.627 kb on - strand, within CA265_RS05715 at 1389.660 kb on + strand, within CA265_RS05715 at 1389.688 kb on - strand, within CA265_RS05715 at 1389.737 kb on + strand, within CA265_RS05715 at 1389.740 kb on - strand, within CA265_RS05715 at 1389.870 kb on - strand, within CA265_RS05715 at 1389.874 kb on - strand, within CA265_RS05715 at 1389.874 kb on - strand, within CA265_RS05715 at 1389.884 kb on + strand, within CA265_RS05715 at 1389.884 kb on + strand, within CA265_RS05715 at 1389.908 kb on + strand, within CA265_RS05715 at 1389.945 kb on - strand, within CA265_RS05715 at 1390.011 kb on - strand, within CA265_RS05715 at 1390.029 kb on - strand, within CA265_RS05715 at 1390.051 kb on - strand, within CA265_RS05715 at 1390.099 kb on - strand, within CA265_RS05715 at 1390.123 kb on - strand, within CA265_RS05715 at 1390.127 kb on + strand, within CA265_RS05715 at 1390.194 kb on + strand, within CA265_RS05715 at 1390.194 kb on + strand, within CA265_RS05715 at 1390.330 kb on - strand, within CA265_RS05715 at 1390.330 kb on - strand, within CA265_RS05715 at 1390.362 kb on + strand, within CA265_RS05715 at 1390.433 kb on - strand, within CA265_RS05715 at 1390.468 kb on + strand, within CA265_RS05715 at 1390.469 kb on - strand, within CA265_RS05715 at 1390.624 kb on + strand at 1390.630 kb on - strand at 1390.630 kb on - strand at 1390.632 kb on + strand at 1390.661 kb on - strand at 1390.734 kb on + strand at 1390.758 kb on - strand at 1390.793 kb on + strand at 1390.798 kb on + strand at 1390.810 kb on + strand at 1390.810 kb on + strand at 1390.876 kb on + strand at 1390.877 kb on - strand at 1390.882 kb on - strand at 1390.920 kb on + strand at 1390.928 kb on + strand at 1391.117 kb on + strand at 1391.301 kb on + strand at 1391.302 kb on - strand at 1391.341 kb on + strand at 1391.386 kb on - strand at 1391.428 kb on - strand at 1391.517 kb on + strand, within CA265_RS05720 at 1391.552 kb on + strand, within CA265_RS05720 at 1391.552 kb on + strand, within CA265_RS05720 at 1391.595 kb on + strand, within CA265_RS05720 at 1391.617 kb on - strand, within CA265_RS05720 at 1391.617 kb on - strand, within CA265_RS05720
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 1,388,634 - CA265_RS05710 0.29 +0.8 1,388,634 - CA265_RS05710 0.29 -1.0 1,388,643 - CA265_RS05710 0.30 -0.2 1,388,644 + CA265_RS05710 0.30 +0.2 1,388,645 - CA265_RS05710 0.30 +0.3 1,388,775 + CA265_RS05710 0.43 -0.1 1,388,801 - CA265_RS05710 0.46 -0.6 1,388,836 + CA265_RS05710 0.49 +0.6 1,388,836 + CA265_RS05710 0.49 +0.3 1,388,901 + CA265_RS05710 0.56 +0.8 1,388,921 + CA265_RS05710 0.58 -0.3 1,388,962 - CA265_RS05710 0.62 -0.2 1,388,963 + CA265_RS05710 0.62 +0.4 1,388,963 + CA265_RS05710 0.62 -0.8 1,388,964 - CA265_RS05710 0.62 -0.4 1,388,964 - CA265_RS05710 0.62 -0.9 1,388,980 + CA265_RS05710 0.64 -0.9 1,389,003 - CA265_RS05710 0.66 -0.7 1,389,010 + CA265_RS05710 0.67 -0.2 1,389,010 + CA265_RS05710 0.67 -0.6 1,389,011 - CA265_RS05710 0.67 +0.5 1,389,011 - CA265_RS05710 0.67 +0.4 1,389,038 + CA265_RS05710 0.69 +1.0 1,389,066 + CA265_RS05710 0.72 +0.4 1,389,133 - CA265_RS05710 0.79 +0.5 1,389,182 + CA265_RS05710 0.84 +0.7 1,389,248 - -0.6 1,389,257 - -0.1 1,389,257 - -0.4 1,389,282 + -0.8 1,389,299 - -0.4 1,389,318 + -0.4 1,389,372 - -0.6 1,389,373 + +0.3 1,389,403 - +1.4 1,389,418 + -0.3 1,389,419 + +0.3 1,389,419 + -0.2 1,389,437 - -0.1 1,389,439 + +0.5 1,389,440 - -1.1 1,389,465 - -0.1 1,389,487 - +0.1 1,389,527 + CA265_RS05715 0.11 -0.5 1,389,611 - CA265_RS05715 0.18 +0.2 1,389,627 - CA265_RS05715 0.19 -0.3 1,389,660 + CA265_RS05715 0.22 +0.7 1,389,688 - CA265_RS05715 0.24 +1.4 1,389,737 + CA265_RS05715 0.28 +0.5 1,389,740 - CA265_RS05715 0.29 +0.3 1,389,870 - CA265_RS05715 0.39 -0.3 1,389,874 - CA265_RS05715 0.39 -0.7 1,389,874 - CA265_RS05715 0.39 -0.3 1,389,884 + CA265_RS05715 0.40 -0.2 1,389,884 + CA265_RS05715 0.40 +0.1 1,389,908 + CA265_RS05715 0.42 -0.2 1,389,945 - CA265_RS05715 0.45 +0.6 1,390,011 - CA265_RS05715 0.50 -0.1 1,390,029 - CA265_RS05715 0.52 -0.6 1,390,051 - CA265_RS05715 0.54 +0.2 1,390,099 - CA265_RS05715 0.57 -0.2 1,390,123 - CA265_RS05715 0.59 +0.3 1,390,127 + CA265_RS05715 0.60 +0.1 1,390,194 + CA265_RS05715 0.65 -0.2 1,390,194 + CA265_RS05715 0.65 -0.4 1,390,330 - CA265_RS05715 0.76 -0.2 1,390,330 - CA265_RS05715 0.76 +0.2 1,390,362 + CA265_RS05715 0.79 +0.4 1,390,433 - CA265_RS05715 0.84 -0.3 1,390,468 + CA265_RS05715 0.87 -1.6 1,390,469 - CA265_RS05715 0.87 +0.1 1,390,624 + -1.0 1,390,630 - -0.0 1,390,630 - +1.9 1,390,632 + -1.3 1,390,661 - -0.6 1,390,734 + -0.2 1,390,758 - +0.7 1,390,793 + -0.6 1,390,798 + -0.8 1,390,810 + -1.0 1,390,810 + -1.0 1,390,876 + -0.8 1,390,877 - -0.1 1,390,882 - -0.7 1,390,920 + -1.3 1,390,928 + -0.5 1,391,117 + -0.8 1,391,301 + -0.3 1,391,302 - +3.8 1,391,341 + -0.0 1,391,386 - +3.5 1,391,428 - +6.1 1,391,517 + CA265_RS05720 0.11 -0.7 1,391,552 + CA265_RS05720 0.13 -1.6 1,391,552 + CA265_RS05720 0.13 -0.4 1,391,595 + CA265_RS05720 0.15 -1.3 1,391,617 - CA265_RS05720 0.16 +4.2 1,391,617 - CA265_RS05720 0.16 +4.1
Or see this region's nucleotide sequence