Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00750 and CA265_RS00755 are separated by 86 nucleotides CA265_RS00755 and CA265_RS00760 are separated by 71 nucleotides CA265_RS00760 and CA265_RS00765 are separated by 128 nucleotides
CA265_RS00750: CA265_RS00750 - hypothetical protein, at 181,608 to 182,831
_RS00750
CA265_RS00755: CA265_RS00755 - 5-dehydro-4-deoxy-D-glucuronate isomerase, at 182,918 to 183,757
_RS00755
CA265_RS00760: CA265_RS00760 - 2-deoxy-D-gluconate 3-dehydrogenase, at 183,829 to 184,593
_RS00760
CA265_RS00765: CA265_RS00765 - hypothetical protein, at 184,722 to 185,567
_RS00765
Position (kb) 183
184
185 Strain fitness (log2 ratio) -2
-1
0
1
2 at 182.830 kb on - strand at 182.838 kb on + strand at 182.839 kb on - strand at 182.841 kb on + strand at 182.846 kb on - strand at 182.853 kb on - strand at 182.962 kb on + strand at 182.963 kb on - strand at 183.036 kb on + strand, within CA265_RS00755 at 183.042 kb on + strand, within CA265_RS00755 at 183.060 kb on - strand, within CA265_RS00755 at 183.060 kb on - strand, within CA265_RS00755 at 183.061 kb on + strand, within CA265_RS00755 at 183.061 kb on + strand, within CA265_RS00755 at 183.061 kb on + strand, within CA265_RS00755 at 183.061 kb on + strand, within CA265_RS00755 at 183.062 kb on - strand, within CA265_RS00755 at 183.062 kb on - strand, within CA265_RS00755 at 183.062 kb on - strand, within CA265_RS00755 at 183.062 kb on - strand, within CA265_RS00755 at 183.062 kb on - strand, within CA265_RS00755 at 183.096 kb on + strand, within CA265_RS00755 at 183.160 kb on + strand, within CA265_RS00755 at 183.161 kb on - strand, within CA265_RS00755 at 183.163 kb on + strand at 183.181 kb on + strand, within CA265_RS00755 at 183.218 kb on + strand, within CA265_RS00755 at 183.219 kb on - strand, within CA265_RS00755 at 183.220 kb on + strand, within CA265_RS00755 at 183.275 kb on - strand, within CA265_RS00755 at 183.298 kb on - strand, within CA265_RS00755 at 183.299 kb on + strand, within CA265_RS00755 at 183.326 kb on - strand, within CA265_RS00755 at 183.326 kb on - strand, within CA265_RS00755 at 183.346 kb on - strand, within CA265_RS00755 at 183.461 kb on - strand, within CA265_RS00755 at 183.508 kb on + strand, within CA265_RS00755 at 183.517 kb on + strand, within CA265_RS00755 at 183.631 kb on + strand, within CA265_RS00755 at 183.632 kb on - strand, within CA265_RS00755 at 183.686 kb on - strand at 183.856 kb on - strand at 183.908 kb on + strand, within CA265_RS00760 at 183.909 kb on - strand, within CA265_RS00760 at 184.156 kb on + strand, within CA265_RS00760 at 184.157 kb on - strand, within CA265_RS00760 at 184.198 kb on - strand, within CA265_RS00760 at 184.269 kb on - strand, within CA265_RS00760 at 184.269 kb on - strand, within CA265_RS00760 at 184.379 kb on + strand, within CA265_RS00760 at 184.399 kb on + strand, within CA265_RS00760 at 184.400 kb on - strand, within CA265_RS00760 at 184.402 kb on - strand, within CA265_RS00760 at 184.416 kb on + strand, within CA265_RS00760 at 184.416 kb on + strand, within CA265_RS00760 at 184.455 kb on + strand, within CA265_RS00760 at 184.456 kb on - strand, within CA265_RS00760 at 184.457 kb on + strand, within CA265_RS00760 at 184.457 kb on + strand, within CA265_RS00760 at 184.458 kb on - strand, within CA265_RS00760 at 184.468 kb on - strand, within CA265_RS00760 at 184.518 kb on - strand at 184.543 kb on + strand at 184.591 kb on + strand at 184.592 kb on - strand at 184.592 kb on - strand at 184.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 182,830 - +0.5 182,838 + +0.4 182,839 - +0.6 182,841 + +0.6 182,846 - -0.6 182,853 - -0.8 182,962 + -0.7 182,963 - +0.6 183,036 + CA265_RS00755 0.14 +0.3 183,042 + CA265_RS00755 0.15 -0.2 183,060 - CA265_RS00755 0.17 +0.3 183,060 - CA265_RS00755 0.17 +0.5 183,061 + CA265_RS00755 0.17 +1.3 183,061 + CA265_RS00755 0.17 +0.5 183,061 + CA265_RS00755 0.17 -2.0 183,061 + CA265_RS00755 0.17 +1.4 183,062 - CA265_RS00755 0.17 -0.3 183,062 - CA265_RS00755 0.17 -0.1 183,062 - CA265_RS00755 0.17 -0.3 183,062 - CA265_RS00755 0.17 -0.3 183,062 - CA265_RS00755 0.17 +0.8 183,096 + CA265_RS00755 0.21 +2.4 183,160 + CA265_RS00755 0.29 +2.0 183,161 - CA265_RS00755 0.29 -0.5 183,163 + +1.4 183,181 + CA265_RS00755 0.31 +0.7 183,218 + CA265_RS00755 0.36 +0.4 183,219 - CA265_RS00755 0.36 -0.6 183,220 + CA265_RS00755 0.36 +0.7 183,275 - CA265_RS00755 0.42 -0.1 183,298 - CA265_RS00755 0.45 +0.0 183,299 + CA265_RS00755 0.45 +0.3 183,326 - CA265_RS00755 0.49 +0.2 183,326 - CA265_RS00755 0.49 +1.2 183,346 - CA265_RS00755 0.51 +0.3 183,461 - CA265_RS00755 0.65 -0.1 183,508 + CA265_RS00755 0.70 -0.7 183,517 + CA265_RS00755 0.71 -0.6 183,631 + CA265_RS00755 0.85 +0.3 183,632 - CA265_RS00755 0.85 +0.1 183,686 - +0.1 183,856 - -0.6 183,908 + CA265_RS00760 0.10 -0.0 183,909 - CA265_RS00760 0.10 -0.5 184,156 + CA265_RS00760 0.43 +0.3 184,157 - CA265_RS00760 0.43 +0.3 184,198 - CA265_RS00760 0.48 +0.2 184,269 - CA265_RS00760 0.58 +1.4 184,269 - CA265_RS00760 0.58 -0.6 184,379 + CA265_RS00760 0.72 +0.9 184,399 + CA265_RS00760 0.75 +0.1 184,400 - CA265_RS00760 0.75 +0.6 184,402 - CA265_RS00760 0.75 -0.1 184,416 + CA265_RS00760 0.77 +1.2 184,416 + CA265_RS00760 0.77 -0.4 184,455 + CA265_RS00760 0.82 +0.6 184,456 - CA265_RS00760 0.82 +0.2 184,457 + CA265_RS00760 0.82 +0.5 184,457 + CA265_RS00760 0.82 -0.3 184,458 - CA265_RS00760 0.82 -0.3 184,468 - CA265_RS00760 0.84 -0.0 184,518 - +0.0 184,543 + +0.4 184,591 + +1.6 184,592 - +0.3 184,592 - +0.6 184,680 - +0.2
Or see this region's nucleotide sequence