Experiment: sodium fluoride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3213 and Shewana3_3214 are separated by 202 nucleotides Shewana3_3214 and Shewana3_3215 are separated by 22 nucleotides Shewana3_3215 and Shewana3_3216 overlap by 4 nucleotides Shewana3_3216 and obgE are separated by 27 nucleotides
Shewana3_3213: Shewana3_3213 - hypothetical protein (RefSeq), at 3,858,222 to 3,858,635
_3213
Shewana3_3214: Shewana3_3214 - dihydrofolate reductase (RefSeq), at 3,858,838 to 3,859,320
_3214
Shewana3_3215: Shewana3_3215 - hypothetical protein (RefSeq), at 3,859,343 to 3,859,816
_3215
Shewana3_3216: Shewana3_3216 - hypothetical protein (RefSeq), at 3,859,813 to 3,860,574
_3216
Shewana3_3217: obgE - GTPase ObgE (RefSeq), at 3,860,602 to 3,861,768
obgE
Position (kb)
3859
3860 Strain fitness (log2 ratio)
-2
-1
0
1 at 3858.418 kb on - strand, within Shewana3_3213 at 3858.821 kb on + strand at 3859.467 kb on - strand, within Shewana3_3215 at 3859.570 kb on - strand, within Shewana3_3215 at 3859.937 kb on - strand, within Shewana3_3216 at 3860.205 kb on - strand, within Shewana3_3216 at 3860.409 kb on - strand, within Shewana3_3216 at 3860.463 kb on - strand, within Shewana3_3216 at 3860.539 kb on - strand at 3860.698 kb on - strand at 3860.728 kb on - strand, within obgE
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 200 mM remove 3,858,418 - Shewana3_3213 0.47 +0.2 3,858,821 + -2.5 3,859,467 - Shewana3_3215 0.26 +0.1 3,859,570 - Shewana3_3215 0.48 -0.4 3,859,937 - Shewana3_3216 0.16 -0.4 3,860,205 - Shewana3_3216 0.51 +0.3 3,860,409 - Shewana3_3216 0.78 -1.5 3,860,463 - Shewana3_3216 0.85 -0.6 3,860,539 - -1.2 3,860,698 - +0.4 3,860,728 - obgE Shewana3_3217 0.11 +0.2
Or see this region's nucleotide sequence