Experiment: sodium fluoride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2330 and Shewana3_2331 are separated by 74 nucleotides Shewana3_2331 and Shewana3_2332 are separated by 6 nucleotides Shewana3_2332 and Shewana3_2333 are separated by 10 nucleotides
Shewana3_2330: Shewana3_2330 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,806,888 to 2,807,898
_2330
Shewana3_2331: Shewana3_2331 - protein tyrosine phosphatase, catalytic region (RefSeq), at 2,807,973 to 2,808,464
_2331
Shewana3_2332: Shewana3_2332 - redox-active disulfide protein 2 (RefSeq), at 2,808,471 to 2,808,707
_2332
Shewana3_2333: Shewana3_2333 - permease (RefSeq), at 2,808,718 to 2,809,716
_2333
Position (kb)
2808
2809 Strain fitness (log2 ratio)
-2
-1
0
1 at 2807.522 kb on + strand, within Shewana3_2330 at 2807.668 kb on - strand, within Shewana3_2330 at 2807.740 kb on + strand at 2807.847 kb on + strand at 2807.855 kb on - strand at 2808.121 kb on - strand, within Shewana3_2331 at 2808.203 kb on - strand, within Shewana3_2331 at 2808.203 kb on - strand, within Shewana3_2331 at 2808.293 kb on + strand, within Shewana3_2331 at 2808.293 kb on + strand, within Shewana3_2331 at 2808.293 kb on + strand, within Shewana3_2331 at 2808.567 kb on + strand, within Shewana3_2332 at 2808.666 kb on - strand, within Shewana3_2332 at 2808.996 kb on - strand, within Shewana3_2333 at 2809.454 kb on - strand, within Shewana3_2333
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 200 mM remove 2,807,522 + Shewana3_2330 0.63 +1.6 2,807,668 - Shewana3_2330 0.77 +1.1 2,807,740 + +1.6 2,807,847 + -1.7 2,807,855 - +0.4 2,808,121 - Shewana3_2331 0.30 -0.2 2,808,203 - Shewana3_2331 0.47 +0.5 2,808,203 - Shewana3_2331 0.47 +0.4 2,808,293 + Shewana3_2331 0.65 -0.4 2,808,293 + Shewana3_2331 0.65 +0.6 2,808,293 + Shewana3_2331 0.65 -1.2 2,808,567 + Shewana3_2332 0.41 -0.7 2,808,666 - Shewana3_2332 0.82 -2.0 2,808,996 - Shewana3_2333 0.28 +0.8 2,809,454 - Shewana3_2333 0.74 -1.1
Or see this region's nucleotide sequence