Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0748 and Shew_0749 are separated by 71 nucleotides Shew_0749 and Shew_0750 are separated by 62 nucleotides Shew_0750 and Shew_0751 are separated by 69 nucleotides Shew_0751 and Shew_0752 are separated by 523 nucleotides
Shew_0748: Shew_0748 - acetyltransferase (RefSeq), at 849,062 to 849,622
_0748
Shew_0749: Shew_0749 - hypothetical protein (RefSeq), at 849,694 to 850,038
_0749
Shew_0750: Shew_0750 - hypothetical protein (RefSeq), at 850,101 to 850,538
_0750
Shew_0751: Shew_0751 - S-adenosylmethionine synthetase (RefSeq), at 850,608 to 851,759
_0751
Shew_0752: Shew_0752 - transketolase (RefSeq), at 852,283 to 854,277
_0752
Position (kb) 850
851
852 Strain fitness (log2 ratio) -1
0
1 at 849.617 kb on + strand at 849.775 kb on - strand, within Shew_0749 at 849.838 kb on - strand, within Shew_0749 at 850.103 kb on + strand at 850.111 kb on + strand at 850.119 kb on - strand at 850.189 kb on - strand, within Shew_0750 at 850.431 kb on + strand, within Shew_0750 at 851.889 kb on + strand at 851.994 kb on + strand at 851.994 kb on + strand at 852.028 kb on + strand at 852.032 kb on - strand at 852.038 kb on + strand at 852.038 kb on + strand at 852.043 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 849,617 + +0.1 849,775 - Shew_0749 0.23 -0.0 849,838 - Shew_0749 0.42 +0.7 850,103 + +0.1 850,111 + -0.3 850,119 - +1.1 850,189 - Shew_0750 0.20 +0.0 850,431 + Shew_0750 0.75 +0.0 851,889 + +0.3 851,994 + -0.6 851,994 + -0.1 852,028 + +0.1 852,032 - -0.1 852,038 + +0.2 852,038 + -0.4 852,043 + +0.3
Or see this region's nucleotide sequence