Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_543 and Pf6N2E2_544 are separated by 153 nucleotides Pf6N2E2_544 and Pf6N2E2_545 are separated by 24 nucleotides Pf6N2E2_545 and Pf6N2E2_546 are separated by 198 nucleotides Pf6N2E2_546 and Pf6N2E2_547 are separated by 221 nucleotides
Pf6N2E2_543 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 612,214 to 613,329
_543
Pf6N2E2_544 - Short-chain dehydrogenase/reductase SDR, at 613,483 to 614,250
_544
Pf6N2E2_545 - Predicted aminoglycoside phosphotransferase, at 614,275 to 615,342
_545
Pf6N2E2_546 - FIG00966904: hypothetical protein, at 615,541 to 615,936
_546
Pf6N2E2_547 - Phophatidylinositol-4-phosphate 5-kinase (EC 2.7.1.68), at 616,158 to 616,700
_547
Position (kb) 614
615
616 Strain fitness (log2 ratio) -2
-1
0
1
2 at 613.444 kb on + strand at 613.444 kb on + strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.529 kb on + strand at 613.529 kb on + strand at 613.553 kb on + strand at 613.553 kb on + strand at 613.554 kb on - strand at 613.769 kb on + strand, within Pf6N2E2_544 at 613.769 kb on + strand, within Pf6N2E2_544 at 613.769 kb on + strand, within Pf6N2E2_544 at 613.770 kb on - strand, within Pf6N2E2_544 at 613.773 kb on + strand, within Pf6N2E2_544 at 613.774 kb on - strand, within Pf6N2E2_544 at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.387 kb on + strand, within Pf6N2E2_545 at 614.387 kb on + strand, within Pf6N2E2_545 at 614.387 kb on + strand, within Pf6N2E2_545 at 614.388 kb on - strand, within Pf6N2E2_545 at 614.388 kb on - strand, within Pf6N2E2_545 at 614.388 kb on - strand, within Pf6N2E2_545 at 614.388 kb on - strand, within Pf6N2E2_545 at 614.426 kb on + strand, within Pf6N2E2_545 at 614.426 kb on + strand, within Pf6N2E2_545 at 614.426 kb on + strand, within Pf6N2E2_545 at 614.426 kb on + strand, within Pf6N2E2_545 at 614.427 kb on - strand, within Pf6N2E2_545 at 614.427 kb on - strand, within Pf6N2E2_545 at 614.432 kb on + strand, within Pf6N2E2_545 at 614.432 kb on + strand, within Pf6N2E2_545 at 614.432 kb on + strand, within Pf6N2E2_545 at 614.432 kb on + strand, within Pf6N2E2_545 at 614.433 kb on - strand, within Pf6N2E2_545 at 614.433 kb on - strand, within Pf6N2E2_545 at 614.433 kb on - strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.477 kb on + strand, within Pf6N2E2_545 at 614.478 kb on - strand, within Pf6N2E2_545 at 614.481 kb on - strand, within Pf6N2E2_545 at 614.693 kb on + strand, within Pf6N2E2_545 at 615.011 kb on + strand, within Pf6N2E2_545 at 615.011 kb on + strand, within Pf6N2E2_545 at 615.012 kb on - strand, within Pf6N2E2_545 at 615.012 kb on - strand, within Pf6N2E2_545 at 615.012 kb on - strand, within Pf6N2E2_545 at 615.053 kb on + strand, within Pf6N2E2_545 at 615.053 kb on + strand, within Pf6N2E2_545 at 615.194 kb on + strand, within Pf6N2E2_545 at 615.194 kb on + strand, within Pf6N2E2_545 at 615.195 kb on - strand, within Pf6N2E2_545 at 615.195 kb on - strand, within Pf6N2E2_545 at 615.272 kb on + strand at 615.360 kb on - strand at 615.429 kb on + strand at 615.429 kb on + strand at 615.430 kb on - strand at 615.430 kb on - strand at 615.430 kb on - strand at 615.430 kb on - strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.501 kb on + strand at 615.859 kb on - strand, within Pf6N2E2_546 at 615.876 kb on + strand, within Pf6N2E2_546 at 615.877 kb on - strand, within Pf6N2E2_546 at 615.938 kb on + strand at 615.963 kb on + strand at 615.963 kb on + strand at 615.963 kb on + strand at 615.963 kb on + strand at 615.964 kb on - strand at 615.964 kb on - strand at 615.964 kb on - strand at 615.964 kb on - strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.972 kb on + strand at 615.973 kb on - strand at 615.973 kb on - strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.985 kb on - strand at 615.985 kb on - strand at 615.985 kb on - strand at 616.092 kb on + strand at 616.160 kb on - strand at 616.160 kb on - strand at 616.160 kb on - strand at 616.160 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 613,444 + +1.3 613,444 + -0.1 613,445 - +1.4 613,445 - +0.1 613,445 - +0.3 613,445 - -0.2 613,445 - +0.5 613,445 - -0.3 613,529 + -0.1 613,529 + -0.5 613,553 + +1.3 613,553 + +2.2 613,554 - -0.5 613,769 + Pf6N2E2_544 0.37 +0.1 613,769 + Pf6N2E2_544 0.37 -0.2 613,769 + Pf6N2E2_544 0.37 -0.4 613,770 - Pf6N2E2_544 0.37 +0.0 613,773 + Pf6N2E2_544 0.38 -0.0 613,774 - Pf6N2E2_544 0.38 +0.6 614,266 + -0.3 614,266 + -0.3 614,266 + +0.6 614,266 + -1.1 614,266 + -0.8 614,266 + +0.3 614,266 + +0.2 614,267 - -0.7 614,267 - +0.6 614,267 - -1.4 614,267 - -0.1 614,267 - +0.5 614,387 + Pf6N2E2_545 0.10 +0.1 614,387 + Pf6N2E2_545 0.10 +0.9 614,387 + Pf6N2E2_545 0.10 -0.3 614,388 - Pf6N2E2_545 0.11 -1.5 614,388 - Pf6N2E2_545 0.11 -0.1 614,388 - Pf6N2E2_545 0.11 +0.7 614,388 - Pf6N2E2_545 0.11 -0.8 614,426 + Pf6N2E2_545 0.14 +0.2 614,426 + Pf6N2E2_545 0.14 +1.0 614,426 + Pf6N2E2_545 0.14 +0.4 614,426 + Pf6N2E2_545 0.14 -0.8 614,427 - Pf6N2E2_545 0.14 +0.3 614,427 - Pf6N2E2_545 0.14 +1.0 614,432 + Pf6N2E2_545 0.15 +0.1 614,432 + Pf6N2E2_545 0.15 -0.1 614,432 + Pf6N2E2_545 0.15 -0.2 614,432 + Pf6N2E2_545 0.15 +0.2 614,433 - Pf6N2E2_545 0.15 +1.2 614,433 - Pf6N2E2_545 0.15 -0.2 614,433 - Pf6N2E2_545 0.15 +0.0 614,435 + Pf6N2E2_545 0.15 +0.5 614,435 + Pf6N2E2_545 0.15 +0.6 614,435 + Pf6N2E2_545 0.15 +0.6 614,435 + Pf6N2E2_545 0.15 +1.1 614,435 + Pf6N2E2_545 0.15 +0.1 614,435 + Pf6N2E2_545 0.15 -0.4 614,435 + Pf6N2E2_545 0.15 -1.5 614,435 + Pf6N2E2_545 0.15 +1.7 614,435 + Pf6N2E2_545 0.15 -0.2 614,436 - Pf6N2E2_545 0.15 -0.8 614,436 - Pf6N2E2_545 0.15 -0.2 614,436 - Pf6N2E2_545 0.15 -0.3 614,436 - Pf6N2E2_545 0.15 -0.0 614,436 - Pf6N2E2_545 0.15 -0.2 614,436 - Pf6N2E2_545 0.15 +0.7 614,436 - Pf6N2E2_545 0.15 +1.4 614,477 + Pf6N2E2_545 0.19 +0.6 614,478 - Pf6N2E2_545 0.19 -0.2 614,481 - Pf6N2E2_545 0.19 +0.8 614,693 + Pf6N2E2_545 0.39 -0.2 615,011 + Pf6N2E2_545 0.69 -0.1 615,011 + Pf6N2E2_545 0.69 +1.6 615,012 - Pf6N2E2_545 0.69 +0.1 615,012 - Pf6N2E2_545 0.69 +0.7 615,012 - Pf6N2E2_545 0.69 +1.2 615,053 + Pf6N2E2_545 0.73 -0.3 615,053 + Pf6N2E2_545 0.73 -0.0 615,194 + Pf6N2E2_545 0.86 +0.8 615,194 + Pf6N2E2_545 0.86 +0.2 615,195 - Pf6N2E2_545 0.86 +0.8 615,195 - Pf6N2E2_545 0.86 -0.2 615,272 + +0.1 615,360 - -0.4 615,429 + -0.5 615,429 + +1.1 615,430 - -0.8 615,430 - +0.0 615,430 - +0.5 615,430 - -0.7 615,431 + -1.2 615,431 + +0.3 615,431 + -0.2 615,431 + +0.2 615,431 + +0.2 615,431 + +0.1 615,431 + -0.0 615,431 + +0.2 615,431 + -0.5 615,431 + +0.5 615,431 + +0.2 615,432 - -0.0 615,432 - -0.3 615,432 - +0.7 615,432 - +0.8 615,432 - -1.3 615,432 - -0.2 615,432 - +0.3 615,432 - +0.7 615,501 + +0.1 615,859 - Pf6N2E2_546 0.80 +0.6 615,876 + Pf6N2E2_546 0.85 +0.6 615,877 - Pf6N2E2_546 0.85 -2.5 615,938 + +0.4 615,963 + -1.0 615,963 + +1.1 615,963 + +0.7 615,963 + -0.9 615,964 - +0.8 615,964 - +0.9 615,964 - +0.8 615,964 - +0.1 615,967 + -0.6 615,967 + +0.2 615,967 + -0.4 615,967 + +0.3 615,967 + +0.5 615,967 + +0.0 615,967 + -0.2 615,968 - +1.1 615,968 - +0.9 615,968 - -1.0 615,968 - +0.5 615,968 - -0.6 615,968 - +0.0 615,968 - +0.0 615,968 - -0.2 615,968 - -0.3 615,968 - +0.6 615,968 - -0.7 615,968 - +0.6 615,968 - +0.4 615,972 + -1.1 615,973 - -0.3 615,973 - +1.2 615,976 + +0.8 615,976 + -0.5 615,976 + -2.3 615,976 + +0.0 615,976 + +0.1 615,976 + -0.4 615,976 + -0.1 615,976 + +1.1 615,976 + +0.8 615,976 + +0.6 615,977 - -1.6 615,977 - +0.2 615,977 - -0.6 615,977 - +0.4 615,977 - +0.3 615,984 + -0.4 615,984 + -1.0 615,984 + +0.2 615,984 + +1.2 615,985 - -0.8 615,985 - -0.2 615,985 - +0.7 616,092 + +0.7 616,160 - -1.1 616,160 - -0.2 616,160 - +0.9 616,160 - +0.8
Or see this region's nucleotide sequence