Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6108 and Pf6N2E2_2020 are separated by 63 nucleotides Pf6N2E2_2020 and Pf6N2E2_2021 are separated by 32 nucleotides Pf6N2E2_2021 and Pf6N2E2_2022 are separated by 68 nucleotides Pf6N2E2_2022 and Pf6N2E2_2023 are separated by 184 nucleotides
Pf6N2E2_6108 - tRNA-Gly-GCC, at 2,408,841 to 2,408,913
_6108
Pf6N2E2_2020 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 2,408,977 to 2,409,537
_2020
Pf6N2E2_2021 - Excinuclease ABC subunit C, at 2,409,570 to 2,411,327
_2021
Pf6N2E2_2022 - BarA-associated response regulator UvrY (= GacA = SirA), at 2,411,396 to 2,411,980
_2022
Pf6N2E2_2023 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 2,412,165 to 2,412,599
_2023
Position (kb)
2409
2410
2411
2412 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2408.620 kb on + strand at 2408.635 kb on + strand at 2408.635 kb on + strand at 2408.636 kb on - strand at 2408.676 kb on + strand at 2408.680 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.713 kb on - strand at 2408.713 kb on - strand at 2408.713 kb on - strand at 2408.713 kb on - strand at 2408.715 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.777 kb on - strand at 2408.935 kb on + strand at 2408.935 kb on + strand at 2408.936 kb on - strand at 2408.936 kb on - strand at 2409.579 kb on - strand at 2409.593 kb on - strand at 2409.593 kb on - strand at 2409.614 kb on + strand at 2409.737 kb on + strand at 2409.737 kb on + strand at 2409.738 kb on - strand at 2409.877 kb on + strand, within Pf6N2E2_2021 at 2409.877 kb on + strand, within Pf6N2E2_2021 at 2410.081 kb on + strand, within Pf6N2E2_2021 at 2410.081 kb on + strand, within Pf6N2E2_2021 at 2410.081 kb on + strand, within Pf6N2E2_2021 at 2410.082 kb on - strand, within Pf6N2E2_2021 at 2410.082 kb on - strand, within Pf6N2E2_2021 at 2410.114 kb on + strand, within Pf6N2E2_2021 at 2410.114 kb on + strand, within Pf6N2E2_2021 at 2410.204 kb on + strand, within Pf6N2E2_2021 at 2410.205 kb on - strand, within Pf6N2E2_2021 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.489 kb on + strand, within Pf6N2E2_2021 at 2410.835 kb on - strand, within Pf6N2E2_2021 at 2410.889 kb on - strand, within Pf6N2E2_2021 at 2410.918 kb on + strand, within Pf6N2E2_2021 at 2410.918 kb on + strand, within Pf6N2E2_2021 at 2410.919 kb on - strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.922 kb on - strand, within Pf6N2E2_2021 at 2410.922 kb on - strand, within Pf6N2E2_2021 at 2411.011 kb on + strand, within Pf6N2E2_2021 at 2411.011 kb on + strand, within Pf6N2E2_2021 at 2411.083 kb on + strand, within Pf6N2E2_2021 at 2411.090 kb on - strand, within Pf6N2E2_2021 at 2411.119 kb on + strand, within Pf6N2E2_2021 at 2411.119 kb on + strand, within Pf6N2E2_2021 at 2411.257 kb on + strand at 2411.258 kb on - strand at 2411.299 kb on + strand at 2411.300 kb on - strand at 2411.480 kb on - strand, within Pf6N2E2_2022 at 2411.490 kb on + strand, within Pf6N2E2_2022 at 2411.490 kb on + strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.540 kb on + strand, within Pf6N2E2_2022 at 2411.540 kb on + strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.668 kb on - strand, within Pf6N2E2_2022 at 2411.668 kb on - strand, within Pf6N2E2_2022 at 2411.951 kb on + strand at 2411.951 kb on + strand at 2411.952 kb on - strand at 2411.986 kb on + strand at 2411.986 kb on + strand at 2411.987 kb on - strand at 2411.987 kb on - strand at 2412.032 kb on - strand at 2412.083 kb on - strand at 2412.106 kb on - strand at 2412.122 kb on + strand at 2412.122 kb on + strand at 2412.123 kb on - strand at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.239 kb on - strand, within Pf6N2E2_2023
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 2,408,620 + -0.4 2,408,635 + +1.5 2,408,635 + +0.5 2,408,636 - +0.8 2,408,676 + -1.0 2,408,680 + -0.9 2,408,712 + +0.4 2,408,712 + +0.9 2,408,712 + -0.1 2,408,712 + +0.5 2,408,712 + +0.3 2,408,712 + +0.9 2,408,712 + -0.1 2,408,713 - -0.8 2,408,713 - +0.2 2,408,713 - +1.0 2,408,713 - -0.4 2,408,715 + +0.8 2,408,776 + +0.2 2,408,776 + -0.2 2,408,776 + +0.4 2,408,776 + +1.9 2,408,776 + -0.7 2,408,776 + -0.1 2,408,777 - -0.2 2,408,935 + -1.8 2,408,935 + +0.3 2,408,936 - +0.9 2,408,936 - -1.5 2,409,579 - -1.4 2,409,593 - -0.3 2,409,593 - -0.9 2,409,614 + -0.4 2,409,737 + -1.7 2,409,737 + +0.5 2,409,738 - -1.4 2,409,877 + Pf6N2E2_2021 0.17 -1.0 2,409,877 + Pf6N2E2_2021 0.17 -0.3 2,410,081 + Pf6N2E2_2021 0.29 -0.4 2,410,081 + Pf6N2E2_2021 0.29 -1.3 2,410,081 + Pf6N2E2_2021 0.29 -1.3 2,410,082 - Pf6N2E2_2021 0.29 -1.4 2,410,082 - Pf6N2E2_2021 0.29 +0.1 2,410,114 + Pf6N2E2_2021 0.31 -0.9 2,410,114 + Pf6N2E2_2021 0.31 -0.8 2,410,204 + Pf6N2E2_2021 0.36 -0.4 2,410,205 - Pf6N2E2_2021 0.36 -0.5 2,410,486 + Pf6N2E2_2021 0.52 +1.2 2,410,486 + Pf6N2E2_2021 0.52 -2.3 2,410,487 - Pf6N2E2_2021 0.52 -0.9 2,410,487 - Pf6N2E2_2021 0.52 +0.2 2,410,487 - Pf6N2E2_2021 0.52 -0.7 2,410,489 + Pf6N2E2_2021 0.52 -1.6 2,410,835 - Pf6N2E2_2021 0.72 -1.9 2,410,889 - Pf6N2E2_2021 0.75 -0.4 2,410,918 + Pf6N2E2_2021 0.77 -0.3 2,410,918 + Pf6N2E2_2021 0.77 +0.3 2,410,919 - Pf6N2E2_2021 0.77 -0.4 2,410,921 + Pf6N2E2_2021 0.77 -1.2 2,410,921 + Pf6N2E2_2021 0.77 -0.9 2,410,921 + Pf6N2E2_2021 0.77 +0.3 2,410,922 - Pf6N2E2_2021 0.77 -2.0 2,410,922 - Pf6N2E2_2021 0.77 -1.4 2,411,011 + Pf6N2E2_2021 0.82 -1.2 2,411,011 + Pf6N2E2_2021 0.82 +0.5 2,411,083 + Pf6N2E2_2021 0.86 -0.2 2,411,090 - Pf6N2E2_2021 0.86 -0.1 2,411,119 + Pf6N2E2_2021 0.88 -0.3 2,411,119 + Pf6N2E2_2021 0.88 -0.9 2,411,257 + -0.1 2,411,258 - -2.4 2,411,299 + -1.4 2,411,300 - +0.0 2,411,480 - Pf6N2E2_2022 0.14 +0.2 2,411,490 + Pf6N2E2_2022 0.16 -0.2 2,411,490 + Pf6N2E2_2022 0.16 +0.0 2,411,491 - Pf6N2E2_2022 0.16 -0.9 2,411,491 - Pf6N2E2_2022 0.16 +0.3 2,411,491 - Pf6N2E2_2022 0.16 -0.8 2,411,491 - Pf6N2E2_2022 0.16 -1.5 2,411,540 + Pf6N2E2_2022 0.25 -0.2 2,411,540 + Pf6N2E2_2022 0.25 -0.3 2,411,541 - Pf6N2E2_2022 0.25 -0.9 2,411,541 - Pf6N2E2_2022 0.25 -0.1 2,411,541 - Pf6N2E2_2022 0.25 -0.3 2,411,667 + Pf6N2E2_2022 0.46 +0.9 2,411,667 + Pf6N2E2_2022 0.46 +0.7 2,411,667 + Pf6N2E2_2022 0.46 +0.3 2,411,667 + Pf6N2E2_2022 0.46 +0.1 2,411,667 + Pf6N2E2_2022 0.46 -0.5 2,411,668 - Pf6N2E2_2022 0.46 +0.6 2,411,668 - Pf6N2E2_2022 0.46 -0.2 2,411,951 + +0.4 2,411,951 + +0.1 2,411,952 - -0.6 2,411,986 + -0.5 2,411,986 + +0.4 2,411,987 - +0.2 2,411,987 - +1.6 2,412,032 - +0.7 2,412,083 - +0.7 2,412,106 - -0.1 2,412,122 + -0.3 2,412,122 + +0.2 2,412,123 - -0.5 2,412,238 + Pf6N2E2_2023 0.17 +0.5 2,412,238 + Pf6N2E2_2023 0.17 +0.5 2,412,238 + Pf6N2E2_2023 0.17 +0.9 2,412,239 - Pf6N2E2_2023 0.17 -0.7
Or see this region's nucleotide sequence