Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_196 and Pf6N2E2_197 overlap by 4 nucleotides Pf6N2E2_197 and Pf6N2E2_198 are separated by 106 nucleotides Pf6N2E2_198 and Pf6N2E2_199 are separated by 9 nucleotides Pf6N2E2_199 and Pf6N2E2_200 are separated by 60 nucleotides
Pf6N2E2_196 - hypothetical protein, at 249,721 to 249,948
_196
Pf6N2E2_197 - Death on curing protein, Doc toxin, at 249,945 to 250,262
_197
Pf6N2E2_198 - hypothetical protein, at 250,369 to 250,527
_198
Pf6N2E2_199 - hypothetical protein, at 250,537 to 250,800
_199
Pf6N2E2_200 - Possible glycosyltransferase, at 250,861 to 251,967
_200
Position (kb)
250
251 Strain fitness (log2 ratio)
-1
0
1
2 at 249.566 kb on + strand at 249.566 kb on + strand at 249.567 kb on - strand at 249.567 kb on - strand at 249.576 kb on + strand at 249.576 kb on + strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.677 kb on - strand at 249.717 kb on + strand at 249.717 kb on + strand at 249.803 kb on - strand, within Pf6N2E2_196 at 249.991 kb on + strand, within Pf6N2E2_197 at 249.991 kb on + strand, within Pf6N2E2_197 at 249.991 kb on + strand, within Pf6N2E2_197 at 249.992 kb on - strand, within Pf6N2E2_197 at 250.008 kb on + strand, within Pf6N2E2_197 at 250.009 kb on - strand, within Pf6N2E2_197 at 250.009 kb on - strand, within Pf6N2E2_197 at 250.012 kb on - strand, within Pf6N2E2_197 at 250.012 kb on - strand, within Pf6N2E2_197 at 250.071 kb on + strand, within Pf6N2E2_197 at 250.071 kb on + strand, within Pf6N2E2_197 at 250.071 kb on + strand, within Pf6N2E2_197 at 250.071 kb on + strand, within Pf6N2E2_197 at 250.072 kb on - strand, within Pf6N2E2_197 at 250.072 kb on - strand, within Pf6N2E2_197 at 250.072 kb on - strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.103 kb on + strand, within Pf6N2E2_197 at 250.104 kb on - strand, within Pf6N2E2_197 at 250.114 kb on - strand, within Pf6N2E2_197 at 250.171 kb on - strand, within Pf6N2E2_197 at 250.182 kb on + strand, within Pf6N2E2_197 at 250.182 kb on + strand, within Pf6N2E2_197 at 250.182 kb on + strand, within Pf6N2E2_197 at 250.182 kb on + strand, within Pf6N2E2_197 at 250.211 kb on - strand, within Pf6N2E2_197 at 250.429 kb on + strand, within Pf6N2E2_198 at 250.430 kb on - strand, within Pf6N2E2_198 at 250.430 kb on - strand, within Pf6N2E2_198 at 250.430 kb on - strand, within Pf6N2E2_198 at 250.455 kb on + strand, within Pf6N2E2_198 at 250.455 kb on + strand, within Pf6N2E2_198 at 250.455 kb on + strand, within Pf6N2E2_198 at 250.456 kb on - strand, within Pf6N2E2_198 at 250.456 kb on - strand, within Pf6N2E2_198 at 250.456 kb on - strand, within Pf6N2E2_198 at 250.480 kb on + strand, within Pf6N2E2_198 at 250.748 kb on + strand, within Pf6N2E2_199 at 250.749 kb on - strand, within Pf6N2E2_199 at 250.749 kb on - strand, within Pf6N2E2_199 at 250.749 kb on - strand, within Pf6N2E2_199 at 250.777 kb on + strand at 250.778 kb on - strand at 250.787 kb on + strand at 250.787 kb on + strand at 250.809 kb on + strand at 250.810 kb on - strand at 250.843 kb on + strand at 251.087 kb on + strand, within Pf6N2E2_200 at 251.088 kb on - strand, within Pf6N2E2_200 at 251.222 kb on - strand, within Pf6N2E2_200 at 251.222 kb on - strand, within Pf6N2E2_200 at 251.422 kb on + strand, within Pf6N2E2_200 at 251.423 kb on - strand, within Pf6N2E2_200 at 251.476 kb on + strand, within Pf6N2E2_200 at 251.476 kb on + strand, within Pf6N2E2_200 at 251.477 kb on - strand, within Pf6N2E2_200 at 251.544 kb on - strand, within Pf6N2E2_200 at 251.574 kb on - strand, within Pf6N2E2_200
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 249,566 + -0.4 249,566 + +0.2 249,567 - +0.7 249,567 - -0.3 249,576 + +1.7 249,576 + +0.2 249,577 - +0.3 249,577 - +2.4 249,577 - -0.1 249,577 - -0.2 249,577 - +1.4 249,577 - +0.2 249,577 - +0.6 249,677 - +0.1 249,717 + -0.8 249,717 + -0.5 249,803 - Pf6N2E2_196 0.36 -0.9 249,991 + Pf6N2E2_197 0.14 +0.4 249,991 + Pf6N2E2_197 0.14 -0.5 249,991 + Pf6N2E2_197 0.14 -0.6 249,992 - Pf6N2E2_197 0.15 -0.6 250,008 + Pf6N2E2_197 0.20 -0.6 250,009 - Pf6N2E2_197 0.20 -0.4 250,009 - Pf6N2E2_197 0.20 -0.5 250,012 - Pf6N2E2_197 0.21 -0.2 250,012 - Pf6N2E2_197 0.21 -1.0 250,071 + Pf6N2E2_197 0.40 +0.6 250,071 + Pf6N2E2_197 0.40 +0.6 250,071 + Pf6N2E2_197 0.40 +0.5 250,071 + Pf6N2E2_197 0.40 +0.8 250,072 - Pf6N2E2_197 0.40 -0.7 250,072 - Pf6N2E2_197 0.40 +0.6 250,072 - Pf6N2E2_197 0.40 +0.2 250,073 + Pf6N2E2_197 0.40 +0.8 250,073 + Pf6N2E2_197 0.40 +1.5 250,073 + Pf6N2E2_197 0.40 +0.2 250,073 + Pf6N2E2_197 0.40 -0.3 250,073 + Pf6N2E2_197 0.40 -0.2 250,074 - Pf6N2E2_197 0.41 -0.5 250,074 - Pf6N2E2_197 0.41 -0.5 250,074 - Pf6N2E2_197 0.41 -1.0 250,074 - Pf6N2E2_197 0.41 +0.3 250,074 - Pf6N2E2_197 0.41 +0.2 250,103 + Pf6N2E2_197 0.50 +0.4 250,104 - Pf6N2E2_197 0.50 -0.4 250,114 - Pf6N2E2_197 0.53 +0.7 250,171 - Pf6N2E2_197 0.71 -1.2 250,182 + Pf6N2E2_197 0.75 +1.0 250,182 + Pf6N2E2_197 0.75 +0.3 250,182 + Pf6N2E2_197 0.75 -0.4 250,182 + Pf6N2E2_197 0.75 -0.3 250,211 - Pf6N2E2_197 0.84 +1.0 250,429 + Pf6N2E2_198 0.38 +0.1 250,430 - Pf6N2E2_198 0.38 +0.2 250,430 - Pf6N2E2_198 0.38 +0.2 250,430 - Pf6N2E2_198 0.38 +0.6 250,455 + Pf6N2E2_198 0.54 +0.4 250,455 + Pf6N2E2_198 0.54 +0.2 250,455 + Pf6N2E2_198 0.54 +0.9 250,456 - Pf6N2E2_198 0.55 -0.1 250,456 - Pf6N2E2_198 0.55 +0.1 250,456 - Pf6N2E2_198 0.55 +0.1 250,480 + Pf6N2E2_198 0.70 -1.3 250,748 + Pf6N2E2_199 0.80 -0.7 250,749 - Pf6N2E2_199 0.80 -1.2 250,749 - Pf6N2E2_199 0.80 -0.4 250,749 - Pf6N2E2_199 0.80 -0.1 250,777 + -0.4 250,778 - +0.6 250,787 + +0.2 250,787 + -1.1 250,809 + +0.2 250,810 - -0.1 250,843 + -0.1 251,087 + Pf6N2E2_200 0.20 +0.5 251,088 - Pf6N2E2_200 0.21 -1.1 251,222 - Pf6N2E2_200 0.33 -0.3 251,222 - Pf6N2E2_200 0.33 -1.2 251,422 + Pf6N2E2_200 0.51 +0.0 251,423 - Pf6N2E2_200 0.51 -1.2 251,476 + Pf6N2E2_200 0.56 -1.2 251,476 + Pf6N2E2_200 0.56 -1.5 251,477 - Pf6N2E2_200 0.56 +0.8 251,544 - Pf6N2E2_200 0.62 -1.7 251,574 - Pf6N2E2_200 0.64 -0.7
Or see this region's nucleotide sequence