Experiment: Temperature shift 30_to_21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01675 and PFLU_RS01680 overlap by 2 nucleotides PFLU_RS01680 and PFLU_RS30350 are separated by 139 nucleotides PFLU_RS30350 and PFLU_RS01685 are separated by 81 nucleotides
PFLU_RS01675: PFLU_RS01675 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 372,782 to 373,237
_RS01675
PFLU_RS01680: PFLU_RS01680 - hypothetical protein, at 373,236 to 373,676
_RS01680
PFLU_RS30350: PFLU_RS30350 - hypothetical protein, at 373,816 to 374,127
_RS30350
PFLU_RS01685: PFLU_RS01685 - nitrogen regulation protein NR(I), at 374,209 to 375,645
_RS01685
Position (kb) 373
374
375 Strain fitness (log2 ratio) -2
-1
0
1
2 at 372.908 kb on + strand, within PFLU_RS01675 at 372.908 kb on + strand, within PFLU_RS01675 at 372.909 kb on - strand, within PFLU_RS01675 at 372.909 kb on - strand, within PFLU_RS01675 at 373.078 kb on - strand, within PFLU_RS01675 at 373.087 kb on - strand, within PFLU_RS01675 at 373.183 kb on + strand, within PFLU_RS01675 at 373.271 kb on + strand at 373.300 kb on + strand, within PFLU_RS01680 at 373.300 kb on + strand, within PFLU_RS01680 at 373.300 kb on + strand, within PFLU_RS01680 at 373.300 kb on + strand, within PFLU_RS01680 at 373.300 kb on + strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.301 kb on - strand, within PFLU_RS01680 at 373.486 kb on + strand, within PFLU_RS01680 at 373.487 kb on - strand, within PFLU_RS01680 at 373.487 kb on - strand, within PFLU_RS01680 at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.674 kb on + strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.675 kb on - strand at 373.730 kb on - strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.747 kb on + strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.748 kb on - strand at 373.760 kb on + strand at 373.760 kb on + strand at 373.760 kb on + strand at 373.760 kb on + strand at 373.761 kb on - strand at 373.761 kb on - strand at 373.761 kb on - strand at 373.787 kb on + strand at 373.787 kb on + strand at 373.832 kb on + strand at 373.858 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.883 kb on + strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 373.884 kb on - strand, within PFLU_RS30350 at 374.010 kb on + strand, within PFLU_RS30350 at 374.011 kb on - strand, within PFLU_RS30350 at 374.011 kb on - strand, within PFLU_RS30350 at 374.011 kb on - strand, within PFLU_RS30350 at 374.011 kb on - strand, within PFLU_RS30350 at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.125 kb on + strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.155 kb on - strand at 374.283 kb on + strand at 374.283 kb on + strand at 374.283 kb on + strand at 374.284 kb on - strand at 374.284 kb on - strand at 374.284 kb on - strand at 374.284 kb on - strand at 374.286 kb on + strand at 374.286 kb on + strand at 374.287 kb on - strand at 374.287 kb on - strand at 374.287 kb on - strand at 374.603 kb on + strand, within PFLU_RS01685 at 374.604 kb on - strand, within PFLU_RS01685 at 374.604 kb on - strand, within PFLU_RS01685 at 374.721 kb on + strand, within PFLU_RS01685 at 374.722 kb on - strand, within PFLU_RS01685 at 374.722 kb on - strand, within PFLU_RS01685 at 374.858 kb on + strand, within PFLU_RS01685
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_21 remove 372,908 + PFLU_RS01675 0.28 +0.4 372,908 + PFLU_RS01675 0.28 -0.5 372,909 - PFLU_RS01675 0.28 -2.6 372,909 - PFLU_RS01675 0.28 -0.1 373,078 - PFLU_RS01675 0.65 -0.2 373,087 - PFLU_RS01675 0.67 +1.2 373,183 + PFLU_RS01675 0.88 -0.9 373,271 + +1.7 373,300 + PFLU_RS01680 0.15 +0.4 373,300 + PFLU_RS01680 0.15 -1.8 373,300 + PFLU_RS01680 0.15 -0.4 373,300 + PFLU_RS01680 0.15 +0.3 373,300 + PFLU_RS01680 0.15 +2.3 373,301 - PFLU_RS01680 0.15 +2.0 373,301 - PFLU_RS01680 0.15 +0.7 373,301 - PFLU_RS01680 0.15 +2.4 373,301 - PFLU_RS01680 0.15 -1.5 373,301 - PFLU_RS01680 0.15 -0.1 373,301 - PFLU_RS01680 0.15 +1.4 373,301 - PFLU_RS01680 0.15 +1.0 373,301 - PFLU_RS01680 0.15 +0.7 373,486 + PFLU_RS01680 0.57 +0.3 373,487 - PFLU_RS01680 0.57 -0.3 373,487 - PFLU_RS01680 0.57 +0.3 373,674 + +0.5 373,674 + +0.4 373,674 + -0.6 373,674 + +0.5 373,674 + +0.8 373,674 + +1.4 373,674 + -1.8 373,674 + +0.2 373,675 - +0.5 373,675 - -0.8 373,675 - +0.4 373,675 - -0.2 373,675 - -1.1 373,675 - +1.0 373,675 - +0.3 373,730 - +0.2 373,747 + -0.5 373,747 + -1.6 373,747 + -0.3 373,747 + +1.4 373,747 + +0.0 373,747 + -0.4 373,747 + +0.8 373,747 + -0.9 373,747 + +1.6 373,747 + +0.6 373,747 + +0.3 373,747 + -1.6 373,747 + +0.4 373,747 + -0.5 373,748 - +0.1 373,748 - +0.5 373,748 - -0.2 373,748 - -0.4 373,748 - +0.3 373,748 - +0.2 373,748 - +0.9 373,748 - -0.1 373,748 - -0.5 373,748 - +0.4 373,748 - +1.5 373,748 - -1.5 373,748 - -0.2 373,748 - +0.2 373,748 - -0.3 373,760 + -0.8 373,760 + -0.2 373,760 + +0.2 373,760 + -0.3 373,761 - -1.1 373,761 - -0.3 373,761 - +1.0 373,787 + -1.2 373,787 + +0.6 373,832 + -0.0 373,858 + PFLU_RS30350 0.13 -1.6 373,883 + PFLU_RS30350 0.21 +0.1 373,883 + PFLU_RS30350 0.21 +0.4 373,883 + PFLU_RS30350 0.21 +1.8 373,883 + PFLU_RS30350 0.21 +0.2 373,883 + PFLU_RS30350 0.21 -0.8 373,883 + PFLU_RS30350 0.21 -0.2 373,883 + PFLU_RS30350 0.21 +0.4 373,884 - PFLU_RS30350 0.22 +1.0 373,884 - PFLU_RS30350 0.22 -0.0 373,884 - PFLU_RS30350 0.22 +0.3 373,884 - PFLU_RS30350 0.22 -0.4 373,884 - PFLU_RS30350 0.22 +0.8 373,884 - PFLU_RS30350 0.22 +0.6 373,884 - PFLU_RS30350 0.22 +0.8 373,884 - PFLU_RS30350 0.22 +0.4 373,884 - PFLU_RS30350 0.22 +1.2 373,884 - PFLU_RS30350 0.22 +0.6 373,884 - PFLU_RS30350 0.22 +2.4 374,010 + PFLU_RS30350 0.62 -0.1 374,011 - PFLU_RS30350 0.62 +1.3 374,011 - PFLU_RS30350 0.62 -0.1 374,011 - PFLU_RS30350 0.62 +0.2 374,011 - PFLU_RS30350 0.62 +1.3 374,125 + +1.0 374,125 + -0.6 374,125 + +0.3 374,125 + -1.2 374,125 + +0.3 374,125 + +0.6 374,125 + -0.5 374,125 + -0.5 374,125 + -0.9 374,125 + +0.2 374,153 - -0.5 374,153 - +0.2 374,153 - +0.1 374,153 - -0.7 374,153 - -0.9 374,153 - +0.8 374,153 - +1.1 374,153 - +0.2 374,153 - +1.4 374,153 - +0.9 374,153 - -0.5 374,155 - +0.3 374,155 - +1.2 374,155 - -0.0 374,155 - -2.0 374,155 - +0.2 374,155 - -0.4 374,155 - +0.3 374,155 - -0.0 374,283 + -0.8 374,283 + -0.6 374,283 + +0.8 374,284 - +0.5 374,284 - +0.9 374,284 - -0.2 374,284 - -1.0 374,286 + -0.6 374,286 + -1.6 374,287 - -0.6 374,287 - -0.2 374,287 - +0.6 374,603 + PFLU_RS01685 0.27 +0.4 374,604 - PFLU_RS01685 0.27 +0.6 374,604 - PFLU_RS01685 0.27 +1.4 374,721 + PFLU_RS01685 0.36 +1.7 374,722 - PFLU_RS01685 0.36 +0.3 374,722 - PFLU_RS01685 0.36 +1.3 374,858 + PFLU_RS01685 0.45 -0.1
Or see this region's nucleotide sequence