Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS10865

Experiment: Temperature shift 30_to_21

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Temperature shift 30_to_21
2,393,666	+				-0.3
2,393,666	+				+0.3
2,393,666	+				+1.1
2,393,667	-				-1.5
2,393,667	-				+2.1
2,393,672	+		PFLU_RS10860	0.10	-0.1
2,393,729	+		PFLU_RS10860	0.18	+0.2
2,393,729	+		PFLU_RS10860	0.18	-0.8
2,393,729	+		PFLU_RS10860	0.18	+0.3
2,393,877	-		PFLU_RS10860	0.39	+0.8
2,393,913	+		PFLU_RS10860	0.43	+0.2
2,393,917	+		PFLU_RS10860	0.44	+0.2
2,393,917	+		PFLU_RS10860	0.44	+0.8
2,393,917	+		PFLU_RS10860	0.44	-0.6
2,393,917	+		PFLU_RS10860	0.44	-0.7
2,393,918	-		PFLU_RS10860	0.44	-0.7
2,393,918	-		PFLU_RS10860	0.44	+1.1
2,394,178	+		PFLU_RS10860	0.80	+1.5
2,394,178	+		PFLU_RS10860	0.80	+3.1
2,394,178	+		PFLU_RS10860	0.80	+0.5
2,394,178	+		PFLU_RS10860	0.80	-0.7
2,394,178	+		PFLU_RS10860	0.80	+0.8
2,394,179	-		PFLU_RS10860	0.80	-0.9
2,394,179	-		PFLU_RS10860	0.80	+1.4
2,394,179	-		PFLU_RS10860	0.80	-0.4
2,394,179	-		PFLU_RS10860	0.80	+0.2
2,394,179	-		PFLU_RS10860	0.80	+0.5
2,394,181	-		PFLU_RS10860	0.80	+1.1
2,394,181	-		PFLU_RS10860	0.80	-1.0
2,394,262	+				+0.3
2,394,262	+				+0.8
2,394,262	+				-0.8
2,394,263	-				-1.9
2,394,263	-				-1.0
2,394,347	-				+0.3
2,394,362	+				-0.0
2,394,362	+				-0.7
2,394,362	+				+0.9
2,394,362	+				-0.5
2,394,362	+				+1.3
2,394,363	-				-0.0
2,394,363	-				+0.8
2,394,363	-				+0.4
2,394,363	-				+0.0
2,394,363	-				-0.5
2,394,385	+				+0.5
2,394,385	+				-0.3
2,394,386	-				+1.6
2,394,401	+				+1.9
2,394,401	+				-0.2
2,394,401	+				+0.1
2,394,401	+				-3.5
2,394,401	+				+0.4
2,394,401	+				+0.5
2,394,401	+				+3.5
2,394,401	+				-1.6
2,394,401	+				-0.3
2,394,401	+				+0.3
2,394,401	+				+0.8
2,394,401	+				+1.0
2,394,402	-				+2.3
2,394,402	-				-0.3
2,394,402	-				+0.1
2,394,402	-				-1.6
2,394,402	-				-0.9
2,394,402	-				+0.4
2,394,402	-				-0.5
2,394,407	+				+0.5
2,394,408	-				+0.3
2,394,408	-				-0.3
2,394,408	-				+0.2
2,394,427	+				+0.5
2,394,428	-				+0.2
2,394,455	+				+0.9
2,394,455	+				-2.0
2,394,477	-				+0.9
2,394,477	-				+0.5
2,394,477	-				+2.5
2,394,491	+				-0.5
2,394,491	+				-1.3
2,394,491	+				-0.6
2,394,491	+				-0.4
2,394,491	+				+1.5
2,394,491	+				+0.3
2,394,492	-				-2.3
2,394,492	-				+0.2
2,394,492	-				+0.4
2,394,518	+				-0.8
2,394,519	-				-1.8
2,394,519	-				-1.8
2,394,540	+				-0.7
2,394,541	-				-0.1
2,394,569	+				+0.3
2,394,569	+				-0.4
2,394,569	+				+1.1
2,394,571	+				-0.3
2,394,571	+				-1.4
2,394,585	+				+0.9
2,394,586	-				-0.6
2,394,586	-				-1.2
2,394,586	-				+0.3
2,394,590	+				+1.0
2,394,590	+				-0.1
2,394,596	+				-0.6
2,394,596	+				+0.2
2,394,596	+				+0.4
2,394,596	+				+1.8
2,394,596	+				-0.5
2,394,597	-				-0.6
2,394,597	-				-0.5
2,394,597	-				+1.3
2,394,597	-				+0.5
2,394,628	+				-1.0
2,394,628	+				-0.0
2,394,628	+				+2.7
2,394,629	-				-0.0
2,394,629	-				+0.4
2,394,629	-				+0.5
2,394,717	+				-1.8
2,394,717	+				+0.7
2,394,718	-				-1.0
2,394,718	-				+1.0
2,394,753	-		PFLU_RS10865	0.11	+1.5
2,394,833	+		PFLU_RS10865	0.19	-1.6
2,394,834	-		PFLU_RS10865	0.19	-0.2
2,394,834	-		PFLU_RS10865	0.19	-0.6
2,394,868	+		PFLU_RS10865	0.22	-0.5
2,394,868	+		PFLU_RS10865	0.22	+0.9
2,395,042	+		PFLU_RS10865	0.38	-0.5
2,395,059	+		PFLU_RS10865	0.40	-0.5
2,395,186	-		PFLU_RS10865	0.52	+1.0
2,395,188	-		PFLU_RS10865	0.52	+1.4
2,395,277	+		PFLU_RS10865	0.60	-1.0
2,395,306	+		PFLU_RS10865	0.63	+0.1
2,395,306	+		PFLU_RS10865	0.63	+2.0
2,395,306	+		PFLU_RS10865	0.63	-0.0
2,395,307	-		PFLU_RS10865	0.63	-0.9
2,395,307	-		PFLU_RS10865	0.63	-0.0
2,395,307	-		PFLU_RS10865	0.63	+1.0
2,395,307	-		PFLU_RS10865	0.63	-0.5
2,395,396	-		PFLU_RS10865	0.71	-1.0
2,395,599	-		PFLU_RS10865	0.90	+0.5
2,395,617	+				+1.8
2,395,618	-				-1.0
2,395,618	-				-2.8
2,395,640	-				+1.6
2,395,665	+				-0.4
2,395,666	-				+1.5
2,395,666	-				+0.4
2,395,666	-				-0.3
2,395,666	-				+0.2
2,395,745	+				-0.2
2,395,745	+				+0.5
2,395,746	-				-0.3
2,395,746	-				-0.0
2,395,746	-				+0.3
2,395,746	-				-0.1
2,395,746	-				-0.8
2,395,746	-				-1.7
2,395,749	+				+0.5
2,395,749	+				+0.5
2,395,749	+				+0.1
2,395,749	+				+1.0
2,395,749	+				+2.1
2,395,750	-				+1.1
2,395,750	-				-0.0
2,395,750	-				-0.0
2,395,750	-				+0.1
2,395,751	+				+0.3
2,395,752	-				+0.8
2,395,758	+				-0.0
2,395,759	-				-0.6
2,395,759	-				-1.5
2,395,759	-				-0.2
2,395,759	-				-1.0
2,395,823	+				+1.7
2,395,824	-				+0.9
2,395,824	-				+2.9
2,395,824	-				-0.0
2,395,849	+		PFLU_RS10870	0.12	+2.9
2,395,849	+		PFLU_RS10870	0.12	+0.7
2,395,849	+		PFLU_RS10870	0.12	+2.0
2,395,849	+		PFLU_RS10870	0.12	+1.0
2,395,850	-		PFLU_RS10870	0.12	+0.7
2,395,879	+		PFLU_RS10870	0.14	+0.0
2,395,879	+		PFLU_RS10870	0.14	-0.1
2,395,879	+		PFLU_RS10870	0.14	-1.2
2,395,880	-		PFLU_RS10870	0.14	+0.7
2,396,020	+		PFLU_RS10870	0.27	+1.5
2,396,020	+		PFLU_RS10870	0.27	+0.5
2,396,020	+		PFLU_RS10870	0.27	+0.5
2,396,020	+		PFLU_RS10870	0.27	+0.2
2,396,021	-		PFLU_RS10870	0.27	+1.4
2,396,021	-		PFLU_RS10870	0.27	-0.4
2,396,021	-		PFLU_RS10870	0.27	+0.3
2,396,021	-		PFLU_RS10870	0.27	+1.0
2,396,136	+		PFLU_RS10870	0.37	+0.5
2,396,136	+		PFLU_RS10870	0.37	+1.3
2,396,137	-		PFLU_RS10870	0.37	-0.3
2,396,137	-		PFLU_RS10870	0.37	-0.0
2,396,153	+		PFLU_RS10870	0.38	+0.4
2,396,233	+		PFLU_RS10870	0.45	-0.0
2,396,233	+		PFLU_RS10870	0.45	+0.5
2,396,233	+		PFLU_RS10870	0.45	+0.6
2,396,234	-		PFLU_RS10870	0.46	+1.1
2,396,234	-		PFLU_RS10870	0.46	-0.5
2,396,234	-		PFLU_RS10870	0.46	+0.3
2,396,234	-		PFLU_RS10870	0.46	-0.4
2,396,290	+		PFLU_RS10870	0.50	+0.9
2,396,291	-		PFLU_RS10870	0.51	-0.8
2,396,301	+		PFLU_RS10870	0.51	-1.2
2,396,301	+		PFLU_RS10870	0.51	-0.5
2,396,302	-		PFLU_RS10870	0.52	+1.1
2,396,345	-		PFLU_RS10870	0.55	-0.3
2,396,347	+		PFLU_RS10870	0.55	-0.0
2,396,423	-		PFLU_RS10870	0.62	+0.1
2,396,423	-		PFLU_RS10870	0.62	-1.5
2,396,548	+		PFLU_RS10870	0.73	-1.8
2,396,548	+		PFLU_RS10870	0.73	-0.4