Experiment: R2A with D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00590 and CA265_RS00595 are separated by 223 nucleotides CA265_RS00595 and CA265_RS00600 are separated by 114 nucleotides CA265_RS00600 and CA265_RS00605 are separated by 135 nucleotides
CA265_RS00590: CA265_RS00590 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 134,934 to 135,935
_RS00590
CA265_RS00595: CA265_RS00595 - hypothetical protein, at 136,159 to 136,356
_RS00595
CA265_RS00600: CA265_RS00600 - recombinase RecA, at 136,471 to 137,484
_RS00600
CA265_RS00605: CA265_RS00605 - endonuclease III, at 137,620 to 138,285
_RS00605
Position (kb) 136
137
138 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 135.934 kb on - strand at 136.148 kb on + strand at 136.148 kb on + strand at 136.158 kb on + strand at 136.158 kb on + strand at 136.158 kb on + strand at 136.159 kb on - strand at 136.308 kb on + strand, within CA265_RS00595 at 136.309 kb on - strand, within CA265_RS00595 at 136.310 kb on + strand, within CA265_RS00595 at 136.310 kb on + strand, within CA265_RS00595 at 136.310 kb on + strand, within CA265_RS00595 at 136.311 kb on - strand, within CA265_RS00595 at 136.384 kb on - strand at 136.402 kb on + strand at 136.403 kb on - strand at 136.427 kb on + strand at 136.433 kb on + strand at 136.450 kb on - strand at 136.454 kb on + strand at 136.498 kb on + strand at 136.575 kb on + strand, within CA265_RS00600 at 136.594 kb on - strand, within CA265_RS00600 at 136.632 kb on + strand, within CA265_RS00600 at 136.806 kb on + strand, within CA265_RS00600 at 136.810 kb on + strand, within CA265_RS00600 at 136.895 kb on - strand, within CA265_RS00600 at 136.911 kb on + strand, within CA265_RS00600 at 136.981 kb on + strand, within CA265_RS00600 at 137.065 kb on + strand, within CA265_RS00600 at 137.083 kb on + strand, within CA265_RS00600 at 137.119 kb on - strand, within CA265_RS00600 at 137.119 kb on - strand, within CA265_RS00600 at 137.149 kb on - strand, within CA265_RS00600 at 137.202 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.327 kb on - strand, within CA265_RS00600 at 137.334 kb on + strand, within CA265_RS00600 at 137.335 kb on - strand, within CA265_RS00600 at 137.407 kb on - strand at 137.442 kb on + strand at 137.530 kb on - strand at 137.530 kb on - strand at 137.669 kb on + strand at 137.669 kb on + strand at 137.681 kb on + strand at 137.682 kb on - strand at 137.682 kb on - strand at 137.729 kb on + strand, within CA265_RS00605 at 137.894 kb on + strand, within CA265_RS00605 at 137.900 kb on - strand, within CA265_RS00605 at 137.975 kb on - strand, within CA265_RS00605 at 137.975 kb on - strand, within CA265_RS00605 at 138.030 kb on + strand, within CA265_RS00605 at 138.133 kb on + strand, within CA265_RS00605 at 138.206 kb on + strand, within CA265_RS00605 at 138.211 kb on + strand, within CA265_RS00605 at 138.212 kb on - strand, within CA265_RS00605 at 138.296 kb on - strand at 138.316 kb on - strand at 138.413 kb on - strand at 138.427 kb on + strand at 138.474 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.12 mg/ml remove 135,934 - -0.2 136,148 + -0.5 136,148 + -0.3 136,158 + -0.2 136,158 + -1.0 136,158 + +1.5 136,159 - +0.5 136,308 + CA265_RS00595 0.75 +0.6 136,309 - CA265_RS00595 0.76 +0.0 136,310 + CA265_RS00595 0.76 -1.1 136,310 + CA265_RS00595 0.76 +0.7 136,310 + CA265_RS00595 0.76 +0.1 136,311 - CA265_RS00595 0.77 +0.2 136,384 - -0.2 136,402 + -0.6 136,403 - -0.3 136,427 + +0.6 136,433 + -0.1 136,450 - +0.8 136,454 + +0.8 136,498 + -1.4 136,575 + CA265_RS00600 0.10 +0.4 136,594 - CA265_RS00600 0.12 -3.1 136,632 + CA265_RS00600 0.16 -1.7 136,806 + CA265_RS00600 0.33 -1.5 136,810 + CA265_RS00600 0.33 -1.8 136,895 - CA265_RS00600 0.42 +0.2 136,911 + CA265_RS00600 0.43 -1.1 136,981 + CA265_RS00600 0.50 -1.6 137,065 + CA265_RS00600 0.59 -3.9 137,083 + CA265_RS00600 0.60 -2.6 137,119 - CA265_RS00600 0.64 -0.5 137,119 - CA265_RS00600 0.64 +0.4 137,149 - CA265_RS00600 0.67 -1.9 137,202 + CA265_RS00600 0.72 -0.9 137,244 + CA265_RS00600 0.76 +0.8 137,244 + CA265_RS00600 0.76 -1.4 137,244 + CA265_RS00600 0.76 -0.4 137,244 + CA265_RS00600 0.76 -3.0 137,327 - CA265_RS00600 0.84 -1.8 137,334 + CA265_RS00600 0.85 -1.5 137,335 - CA265_RS00600 0.85 -1.6 137,407 - +0.1 137,442 + -2.3 137,530 - -0.2 137,530 - -0.2 137,669 + +1.2 137,669 + +0.1 137,681 + -1.1 137,682 - -0.5 137,682 - -0.8 137,729 + CA265_RS00605 0.16 -0.5 137,894 + CA265_RS00605 0.41 +1.1 137,900 - CA265_RS00605 0.42 -1.5 137,975 - CA265_RS00605 0.53 +0.2 137,975 - CA265_RS00605 0.53 -0.4 138,030 + CA265_RS00605 0.62 -0.8 138,133 + CA265_RS00605 0.77 -0.6 138,206 + CA265_RS00605 0.88 -0.2 138,211 + CA265_RS00605 0.89 +0.2 138,212 - CA265_RS00605 0.89 +0.1 138,296 - +0.4 138,316 - -0.6 138,413 - +0.5 138,427 + -0.6 138,474 + -0.1
Or see this region's nucleotide sequence