Experiment: CDM1 without Adenine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14250 and BBR_RS14255 are separated by 72 nucleotides BBR_RS14255 and BBR_RS14260 are separated by 90 nucleotides BBR_RS14260 and BBR_RS14265 are separated by 157 nucleotides
BBR_RS14250: BBR_RS14250 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 972,068 to 972,577
_RS14250
BBR_RS14255: BBR_RS14255 - metal ABC transporter permease, at 972,650 to 973,483
_RS14255
BBR_RS14260: BBR_RS14260 - ABC transporter ATP-binding protein, at 973,574 to 974,446
_RS14260
BBR_RS14265: BBR_RS14265 - Solute-binding protein of ABC transporter system for metals, at 974,604 to 975,776
_RS14265
Position (kb) 973
974
975 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 972.662 kb on + strand at 972.672 kb on + strand at 972.692 kb on - strand at 972.692 kb on - strand at 973.555 kb on - strand at 973.560 kb on - strand at 973.595 kb on + strand at 973.727 kb on - strand, within BBR_RS14260 at 973.761 kb on - strand, within BBR_RS14260 at 974.456 kb on - strand at 974.523 kb on - strand at 974.562 kb on + strand at 974.584 kb on - strand at 974.608 kb on - strand at 974.628 kb on - strand at 974.646 kb on - strand at 974.648 kb on + strand at 974.659 kb on + strand at 974.662 kb on - strand at 974.662 kb on - strand at 974.682 kb on - strand at 974.738 kb on - strand, within BBR_RS14265 at 974.776 kb on - strand, within BBR_RS14265 at 974.834 kb on + strand, within BBR_RS14265 at 974.841 kb on + strand, within BBR_RS14265 at 974.860 kb on - strand, within BBR_RS14265 at 974.893 kb on - strand, within BBR_RS14265 at 974.903 kb on + strand, within BBR_RS14265 at 974.915 kb on - strand, within BBR_RS14265 at 974.926 kb on + strand, within BBR_RS14265 at 975.051 kb on + strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.113 kb on - strand, within BBR_RS14265 at 975.151 kb on + strand, within BBR_RS14265 at 975.159 kb on + strand, within BBR_RS14265 at 975.191 kb on - strand, within BBR_RS14265 at 975.288 kb on + strand, within BBR_RS14265 at 975.295 kb on + strand, within BBR_RS14265 at 975.296 kb on - strand, within BBR_RS14265 at 975.324 kb on + strand, within BBR_RS14265
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Adenine remove 972,662 + +0.5 972,672 + +0.9 972,692 - +0.9 972,692 - +2.3 973,555 - +0.5 973,560 - -2.2 973,595 + +0.6 973,727 - BBR_RS14260 0.18 -2.4 973,761 - BBR_RS14260 0.21 -2.0 974,456 - +1.4 974,523 - -0.4 974,562 + -0.4 974,584 - +1.6 974,608 - +1.3 974,628 - +0.5 974,646 - +1.1 974,648 + -4.0 974,659 + -0.2 974,662 - -0.2 974,662 - +0.7 974,682 - -0.3 974,738 - BBR_RS14265 0.11 -0.4 974,776 - BBR_RS14265 0.15 +0.8 974,834 + BBR_RS14265 0.20 -2.3 974,841 + BBR_RS14265 0.20 -2.5 974,860 - BBR_RS14265 0.22 +0.6 974,893 - BBR_RS14265 0.25 +0.2 974,903 + BBR_RS14265 0.25 +0.0 974,915 - BBR_RS14265 0.27 -1.7 974,926 + BBR_RS14265 0.27 -3.1 975,051 + BBR_RS14265 0.38 -2.0 975,102 - BBR_RS14265 0.42 -0.9 975,102 - BBR_RS14265 0.42 +0.2 975,113 - BBR_RS14265 0.43 -2.1 975,151 + BBR_RS14265 0.47 -2.1 975,159 + BBR_RS14265 0.47 -2.7 975,191 - BBR_RS14265 0.50 -1.7 975,288 + BBR_RS14265 0.58 -3.0 975,295 + BBR_RS14265 0.59 -3.1 975,296 - BBR_RS14265 0.59 -2.4 975,324 + BBR_RS14265 0.61 -2.6
Or see this region's nucleotide sequence