Experiment: CDM1 without Adenine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14075 and BBR_RS14080 overlap by 4 nucleotides BBR_RS14080 and BBR_RS14085 overlap by 4 nucleotides BBR_RS14085 and BBR_RS20820 are separated by 20 nucleotides
BBR_RS14075: BBR_RS14075 - glutamate-5-semialdehyde dehydrogenase, at 933,175 to 934,470
_RS14075
BBR_RS14080: BBR_RS14080 - hypothetical protein, at 934,467 to 935,030
_RS14080
BBR_RS14085: BBR_RS14085 - nicotinate-nucleotide adenylyltransferase, at 935,027 to 935,755
_RS14085
BBR_RS20820: BBR_RS20820 - beta-glucosidase, at 935,776 to 936,989
_RS20820
Position (kb) 934
935
936 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 933.487 kb on - strand, within BBR_RS14075 at 933.494 kb on + strand, within BBR_RS14075 at 933.494 kb on + strand, within BBR_RS14075 at 933.494 kb on + strand, within BBR_RS14075 at 933.671 kb on + strand, within BBR_RS14075 at 933.718 kb on + strand, within BBR_RS14075 at 933.741 kb on + strand, within BBR_RS14075 at 933.793 kb on + strand, within BBR_RS14075 at 933.812 kb on + strand, within BBR_RS14075 at 933.832 kb on + strand, within BBR_RS14075 at 933.837 kb on + strand, within BBR_RS14075 at 933.890 kb on + strand, within BBR_RS14075 at 933.951 kb on + strand, within BBR_RS14075 at 933.951 kb on + strand, within BBR_RS14075 at 933.953 kb on + strand, within BBR_RS14075 at 934.044 kb on + strand, within BBR_RS14075 at 934.044 kb on + strand, within BBR_RS14075 at 934.044 kb on + strand, within BBR_RS14075 at 934.044 kb on + strand, within BBR_RS14075 at 934.044 kb on + strand, within BBR_RS14075 at 934.044 kb on + strand, within BBR_RS14075 at 934.052 kb on - strand, within BBR_RS14075 at 934.127 kb on - strand, within BBR_RS14075 at 934.137 kb on + strand, within BBR_RS14075 at 934.180 kb on - strand, within BBR_RS14075 at 934.300 kb on + strand, within BBR_RS14075 at 934.358 kb on + strand at 934.410 kb on - strand at 934.436 kb on + strand at 934.453 kb on + strand at 934.453 kb on + strand at 934.453 kb on + strand at 934.461 kb on - strand at 934.461 kb on - strand at 934.461 kb on - strand at 934.461 kb on - strand at 934.461 kb on - strand at 934.495 kb on + strand at 934.495 kb on + strand at 934.718 kb on + strand, within BBR_RS14080 at 934.718 kb on + strand, within BBR_RS14080 at 934.778 kb on + strand, within BBR_RS14080 at 934.842 kb on + strand, within BBR_RS14080 at 935.068 kb on + strand at 935.073 kb on + strand at 935.073 kb on + strand at 935.076 kb on - strand at 935.083 kb on + strand at 935.088 kb on + strand at 935.091 kb on - strand at 935.158 kb on + strand, within BBR_RS14085 at 935.165 kb on + strand, within BBR_RS14085 at 935.917 kb on + strand, within BBR_RS20820 at 935.982 kb on - strand, within BBR_RS20820
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Adenine remove 933,487 - BBR_RS14075 0.24 +0.6 933,494 + BBR_RS14075 0.25 -0.9 933,494 + BBR_RS14075 0.25 -1.4 933,494 + BBR_RS14075 0.25 +2.0 933,671 + BBR_RS14075 0.38 -1.5 933,718 + BBR_RS14075 0.42 -2.8 933,741 + BBR_RS14075 0.44 -1.3 933,793 + BBR_RS14075 0.48 -1.8 933,812 + BBR_RS14075 0.49 -3.4 933,832 + BBR_RS14075 0.51 -2.6 933,837 + BBR_RS14075 0.51 -0.7 933,890 + BBR_RS14075 0.55 -1.5 933,951 + BBR_RS14075 0.60 -3.1 933,951 + BBR_RS14075 0.60 -2.4 933,953 + BBR_RS14075 0.60 -2.7 934,044 + BBR_RS14075 0.67 -3.5 934,044 + BBR_RS14075 0.67 -3.6 934,044 + BBR_RS14075 0.67 -1.6 934,044 + BBR_RS14075 0.67 -2.9 934,044 + BBR_RS14075 0.67 -1.7 934,044 + BBR_RS14075 0.67 -1.8 934,052 - BBR_RS14075 0.68 -0.0 934,127 - BBR_RS14075 0.73 -0.7 934,137 + BBR_RS14075 0.74 -1.0 934,180 - BBR_RS14075 0.78 -0.9 934,300 + BBR_RS14075 0.87 -2.4 934,358 + -3.7 934,410 - -1.3 934,436 + -2.8 934,453 + +1.9 934,453 + +2.5 934,453 + -0.8 934,461 - +0.4 934,461 - -1.1 934,461 - +0.5 934,461 - -1.9 934,461 - -1.4 934,495 + +4.8 934,495 + +4.6 934,718 + BBR_RS14080 0.45 +5.4 934,718 + BBR_RS14080 0.45 +4.4 934,778 + BBR_RS14080 0.55 +5.0 934,842 + BBR_RS14080 0.66 +3.8 935,068 + +1.3 935,073 + +2.9 935,073 + +3.1 935,076 - +1.1 935,083 + -3.5 935,088 + +3.0 935,091 - -1.7 935,158 + BBR_RS14085 0.18 +2.3 935,165 + BBR_RS14085 0.19 +1.7 935,917 + BBR_RS20820 0.12 -1.1 935,982 - BBR_RS20820 0.17 +1.0
Or see this region's nucleotide sequence