Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2722 and Shew_2723 are separated by 46 nucleotides Shew_2723 and Shew_2724 are separated by 94 nucleotides Shew_2724 and Shew_2725 overlap by 7 nucleotides
Shew_2722: Shew_2722 - hypothetical protein (RefSeq), at 3,222,236 to 3,222,943
_2722
Shew_2723: Shew_2723 - NUDIX hydrolase (RefSeq), at 3,222,990 to 3,223,526
_2723
Shew_2724: Shew_2724 - indigoidine synthase A family protein (RefSeq), at 3,223,621 to 3,224,535
_2724
Shew_2725: Shew_2725 - ribokinase-like domain-containing protein (RefSeq), at 3,224,529 to 3,225,620
_2725
Position (kb)
3223
3224
3225 Strain fitness (log2 ratio)
-1
0
1 at 3222.625 kb on + strand, within Shew_2722 at 3222.633 kb on - strand, within Shew_2722 at 3223.193 kb on + strand, within Shew_2723 at 3223.247 kb on + strand, within Shew_2723 at 3223.911 kb on + strand, within Shew_2724 at 3223.911 kb on + strand, within Shew_2724 at 3223.919 kb on - strand, within Shew_2724 at 3224.291 kb on + strand, within Shew_2724 at 3224.299 kb on - strand, within Shew_2724 at 3224.402 kb on - strand, within Shew_2724 at 3224.458 kb on + strand at 3224.875 kb on + strand, within Shew_2725 at 3225.205 kb on - strand, within Shew_2725 at 3225.347 kb on + strand, within Shew_2725 at 3225.469 kb on - strand, within Shew_2725
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 3,222,625 + Shew_2722 0.55 +0.4 3,222,633 - Shew_2722 0.56 +0.4 3,223,193 + Shew_2723 0.38 +0.5 3,223,247 + Shew_2723 0.48 +0.5 3,223,911 + Shew_2724 0.32 +1.2 3,223,911 + Shew_2724 0.32 +1.0 3,223,919 - Shew_2724 0.33 +0.2 3,224,291 + Shew_2724 0.73 +0.5 3,224,299 - Shew_2724 0.74 +0.3 3,224,402 - Shew_2724 0.85 +0.7 3,224,458 + +0.5 3,224,875 + Shew_2725 0.32 -0.4 3,225,205 - Shew_2725 0.62 +0.2 3,225,347 + Shew_2725 0.75 +0.2 3,225,469 - Shew_2725 0.86 -0.0
Or see this region's nucleotide sequence