Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2366 and Shew_2367 are separated by 102 nucleotides Shew_2367 and Shew_2368 are separated by 138 nucleotides Shew_2368 and Shew_2369 are separated by 0 nucleotides
Shew_2366: Shew_2366 - 3-oxoacyl-(acyl carrier protein) synthase III (RefSeq), at 2,767,400 to 2,768,467
_2366
Shew_2367: Shew_2367 - hypothetical protein (RefSeq), at 2,768,570 to 2,769,160
_2367
Shew_2368: Shew_2368 - exonuclease, RNase T and DNA polymerase III (RefSeq), at 2,769,299 to 2,769,979
_2368
Shew_2369: Shew_2369 - cyclic nucleotide-binding protein (RefSeq), at 2,769,980 to 2,771,827
_2369
Position (kb)
2768
2769
2770 Strain fitness (log2 ratio)
-1
0
1 at 2767.776 kb on - strand, within Shew_2366 at 2768.041 kb on - strand, within Shew_2366 at 2768.565 kb on - strand at 2768.668 kb on + strand, within Shew_2367 at 2768.880 kb on - strand, within Shew_2367 at 2768.994 kb on + strand, within Shew_2367 at 2769.181 kb on + strand at 2769.189 kb on - strand at 2770.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 2,767,776 - Shew_2366 0.35 +0.9 2,768,041 - Shew_2366 0.60 +1.0 2,768,565 - -0.4 2,768,668 + Shew_2367 0.17 +1.0 2,768,880 - Shew_2367 0.52 +1.4 2,768,994 + Shew_2367 0.72 -0.2 2,769,181 + +0.4 2,769,189 - -0.3 2,770,041 - +0.2
Or see this region's nucleotide sequence