Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1230 and Shew_1231 are separated by 434 nucleotides Shew_1231 and Shew_1232 overlap by 1 nucleotides Shew_1232 and Shew_1233 are separated by 50 nucleotides
Shew_1230: Shew_1230 - phosphoribosylaminoimidazole carboxylase, catalytic subunit (RefSeq), at 1,429,520 to 1,430,029
_1230
Shew_1231: Shew_1231 - ECF subfamily RNA polymerase sigma-24 factor (RefSeq), at 1,430,464 to 1,431,057
_1231
Shew_1232: Shew_1232 - hypothetical protein (RefSeq), at 1,431,057 to 1,431,824
_1232
Shew_1233: Shew_1233 - hypothetical protein (RefSeq), at 1,431,875 to 1,432,366
_1233
Position (kb)
1430
1431
1432 Strain fitness (log2 ratio)
-1
0
1
2 at 1429.538 kb on + strand at 1429.642 kb on + strand, within Shew_1230 at 1429.642 kb on + strand, within Shew_1230 at 1429.845 kb on - strand, within Shew_1230 at 1430.409 kb on + strand at 1430.614 kb on - strand, within Shew_1231 at 1430.677 kb on - strand, within Shew_1231 at 1431.131 kb on + strand at 1431.618 kb on + strand, within Shew_1232 at 1431.618 kb on + strand, within Shew_1232 at 1431.626 kb on - strand, within Shew_1232 at 1431.694 kb on - strand, within Shew_1232 at 1431.987 kb on - strand, within Shew_1233
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 1,429,538 + +0.2 1,429,642 + Shew_1230 0.24 +0.2 1,429,642 + Shew_1230 0.24 +0.1 1,429,845 - Shew_1230 0.64 +1.3 1,430,409 + -0.9 1,430,614 - Shew_1231 0.25 +0.5 1,430,677 - Shew_1231 0.36 +2.0 1,431,131 + -0.5 1,431,618 + Shew_1232 0.73 -0.1 1,431,618 + Shew_1232 0.73 +0.4 1,431,626 - Shew_1232 0.74 -0.1 1,431,694 - Shew_1232 0.83 +1.1 1,431,987 - Shew_1233 0.23 -0.2
Or see this region's nucleotide sequence