Strain Fitness in Shewanella loihica PV-4 around Shew_1024

Experiment: nitrite 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_1022 and Shew_1023 are separated by 146 nucleotidesShew_1023 and Shew_1024 are separated by 1 nucleotidesShew_1024 and Shew_1025 overlap by 4 nucleotidesShew_1025 and Shew_1026 are separated by 115 nucleotides Shew_1022: Shew_1022 - tyrosyl-tRNA synthetase (RefSeq), at 1,181,413 to 1,182,609 _1022 Shew_1023: Shew_1023 - hypothetical protein (RefSeq), at 1,182,756 to 1,183,235 _1023 Shew_1024: Shew_1024 - hypothetical protein (RefSeq), at 1,183,237 to 1,183,857 _1024 Shew_1025: Shew_1025 - hypothetical protein (RefSeq), at 1,183,854 to 1,184,243 _1025 Shew_1026: Shew_1026 - cobalamin biosynthesis protein CbiB (RefSeq), at 1,184,359 to 1,185,342 _1026 Position (kb) 1183 1184Strain fitness (log2 ratio) -1 0 1at 1182.871 kb on - strand, within Shew_1023at 1183.044 kb on + strand, within Shew_1023at 1183.065 kb on - strand, within Shew_1023at 1183.233 kb on + strandat 1183.373 kb on - strand, within Shew_1024at 1183.373 kb on - strand, within Shew_1024at 1183.588 kb on + strand, within Shew_1024at 1183.750 kb on + strand, within Shew_1024at 1183.925 kb on + strand, within Shew_1025at 1183.925 kb on + strand, within Shew_1025at 1184.490 kb on - strand, within Shew_1026at 1184.562 kb on - strand, within Shew_1026at 1184.768 kb on + strand, within Shew_1026

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 200 mM
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1,182,871 - Shew_1023 0.24 -0.5
1,183,044 + Shew_1023 0.60 +1.4
1,183,065 - Shew_1023 0.64 +0.4
1,183,233 + -1.6
1,183,373 - Shew_1024 0.22 +0.5
1,183,373 - Shew_1024 0.22 +0.1
1,183,588 + Shew_1024 0.57 +0.1
1,183,750 + Shew_1024 0.83 -0.2
1,183,925 + Shew_1025 0.18 -1.3
1,183,925 + Shew_1025 0.18 -0.1
1,184,490 - Shew_1026 0.13 +0.1
1,184,562 - Shew_1026 0.21 +0.7
1,184,768 + Shew_1026 0.42 +0.2

Or see this region's nucleotide sequence