Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0315 and Shew_0316 overlap by 4 nucleotides Shew_0316 and Shew_0317 are separated by 55 nucleotides Shew_0317 and hemC are separated by 2 nucleotides
Shew_0315: Shew_0315 - HemY domain-containing protein (RefSeq), at 360,568 to 361,734
_0315
Shew_0316: Shew_0316 - protein of unknown function DUF513, hemX (RefSeq), at 361,731 to 362,873
_0316
Shew_0317: Shew_0317 - uroporphyrinogen III synthase HEM4 (RefSeq), at 362,929 to 363,660
_0317
Shew_0318: hemC - porphobilinogen deaminase (RefSeq), at 363,663 to 364,616
hemC
Position (kb) 361
362
363 Strain fitness (log2 ratio) -1
0
1 at 361.178 kb on + strand, within Shew_0315 at 361.453 kb on + strand, within Shew_0315 at 361.770 kb on + strand at 361.962 kb on - strand, within Shew_0316 at 362.185 kb on - strand, within Shew_0316 at 362.185 kb on - strand, within Shew_0316 at 362.234 kb on - strand, within Shew_0316 at 362.452 kb on + strand, within Shew_0316 at 362.558 kb on + strand, within Shew_0316 at 362.884 kb on + strand at 362.949 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 361,178 + Shew_0315 0.52 +0.4 361,453 + Shew_0315 0.76 +0.8 361,770 + +0.5 361,962 - Shew_0316 0.20 +1.5 362,185 - Shew_0316 0.40 +0.2 362,185 - Shew_0316 0.40 +0.8 362,234 - Shew_0316 0.44 +0.5 362,452 + Shew_0316 0.63 +1.2 362,558 + Shew_0316 0.72 +1.1 362,884 + +0.8 362,949 - +0.7
Or see this region's nucleotide sequence