Experiment: nitrite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0284 and Shew_0285 are separated by 28 nucleotides Shew_0285 and Shew_0286 are separated by 197 nucleotides
Shew_0284: Shew_0284 - hypothetical protein (RefSeq), at 302,166 to 303,491
_0284
Shew_0285: Shew_0285 - diguanylate cyclase (RefSeq), at 303,520 to 304,401
_0285
Shew_0286: Shew_0286 - extracellular solute-binding protein (RefSeq), at 304,599 to 305,408
_0286
Position (kb) 303
304
305 Strain fitness (log2 ratio) -2
-1
0
1
2 at 302.621 kb on + strand, within Shew_0284 at 302.650 kb on - strand, within Shew_0284 at 302.698 kb on + strand, within Shew_0284 at 302.859 kb on - strand, within Shew_0284 at 303.036 kb on + strand, within Shew_0284 at 303.046 kb on + strand, within Shew_0284 at 303.046 kb on + strand, within Shew_0284 at 303.046 kb on + strand, within Shew_0284 at 303.257 kb on - strand, within Shew_0284 at 303.279 kb on + strand, within Shew_0284 at 303.388 kb on + strand at 303.542 kb on - strand at 303.561 kb on + strand at 303.584 kb on - strand at 303.584 kb on + strand at 303.784 kb on - strand, within Shew_0285 at 304.174 kb on + strand, within Shew_0285 at 304.786 kb on + strand, within Shew_0286 at 304.794 kb on - strand, within Shew_0286 at 305.014 kb on - strand, within Shew_0286 at 305.014 kb on - strand, within Shew_0286 at 305.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 200 mM remove 302,621 + Shew_0284 0.34 +0.1 302,650 - Shew_0284 0.37 +2.3 302,698 + Shew_0284 0.40 +0.0 302,859 - Shew_0284 0.52 +1.0 303,036 + Shew_0284 0.66 +0.1 303,046 + Shew_0284 0.66 +0.4 303,046 + Shew_0284 0.66 +0.2 303,046 + Shew_0284 0.66 -0.5 303,257 - Shew_0284 0.82 -0.1 303,279 + Shew_0284 0.84 -0.3 303,388 + -1.3 303,542 - +0.2 303,561 + +0.4 303,584 - -0.2 303,584 + +0.1 303,784 - Shew_0285 0.30 -2.3 304,174 + Shew_0285 0.74 -0.3 304,786 + Shew_0286 0.23 +0.0 304,794 - Shew_0286 0.24 +0.0 305,014 - Shew_0286 0.51 +0.1 305,014 - Shew_0286 0.51 +0.5 305,373 + -0.7
Or see this region's nucleotide sequence