Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20985 and CA265_RS20990 are separated by 148 nucleotides CA265_RS20990 and CA265_RS20995 are separated by 7 nucleotides CA265_RS20995 and CA265_RS21000 are separated by 44 nucleotides
CA265_RS20985: CA265_RS20985 - hypothetical protein, at 5,170,170 to 5,171,864
_RS20985
CA265_RS20990: CA265_RS20990 - sugar transporter, at 5,172,013 to 5,172,462
_RS20990
CA265_RS20995: CA265_RS20995 - biotin--[acetyl-CoA-carboxylase] ligase, at 5,172,470 to 5,173,246
_RS20995
CA265_RS21000: CA265_RS21000 - ribosome silencing factor, at 5,173,291 to 5,173,665
_RS21000
Position (kb)
5172
5173 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5171.851 kb on - strand at 5171.908 kb on + strand at 5172.014 kb on + strand at 5172.064 kb on - strand, within CA265_RS20990 at 5172.109 kb on - strand, within CA265_RS20990 at 5172.320 kb on - strand, within CA265_RS20990 at 5172.322 kb on - strand, within CA265_RS20990 at 5172.472 kb on - strand at 5173.220 kb on + strand at 5173.262 kb on + strand at 5173.307 kb on + strand at 5173.320 kb on + strand at 5173.352 kb on + strand, within CA265_RS21000 at 5173.374 kb on + strand, within CA265_RS21000 at 5173.374 kb on + strand, within CA265_RS21000 at 5173.425 kb on + strand, within CA265_RS21000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 5,171,851 - -0.2 5,171,908 + -1.7 5,172,014 + +0.9 5,172,064 - CA265_RS20990 0.11 -2.9 5,172,109 - CA265_RS20990 0.21 +0.1 5,172,320 - CA265_RS20990 0.68 -0.0 5,172,322 - CA265_RS20990 0.69 -1.4 5,172,472 - -0.2 5,173,220 + -2.5 5,173,262 + +0.6 5,173,307 + +0.1 5,173,320 + +0.4 5,173,352 + CA265_RS21000 0.16 -0.0 5,173,374 + CA265_RS21000 0.22 -0.8 5,173,374 + CA265_RS21000 0.22 -0.5 5,173,425 + CA265_RS21000 0.36 -0.5
Or see this region's nucleotide sequence