Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20255 and CA265_RS20260 are separated by 100 nucleotides CA265_RS20260 and CA265_RS20265 overlap by 9 nucleotides CA265_RS20265 and CA265_RS20270 are separated by 331 nucleotides
CA265_RS20255: CA265_RS20255 - trans-aconitate 2-methyltransferase, at 5,004,118 to 5,004,924
_RS20255
CA265_RS20260: CA265_RS20260 - tRNA-Arg, at 5,005,025 to 5,005,100
_RS20260
CA265_RS20265: CA265_RS20265 - excinuclease ABC subunit C, at 5,005,092 to 5,005,337
_RS20265
CA265_RS20270: CA265_RS20270 - methionine--tRNA ligase, at 5,005,669 to 5,007,777
_RS20270
Position (kb) 5005
5006 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5004.126 kb on + strand at 5004.190 kb on + strand at 5004.197 kb on + strand at 5004.200 kb on - strand, within CA265_RS20255 at 5004.277 kb on + strand, within CA265_RS20255 at 5004.277 kb on + strand, within CA265_RS20255 at 5004.278 kb on - strand, within CA265_RS20255 at 5004.301 kb on + strand, within CA265_RS20255 at 5004.301 kb on + strand, within CA265_RS20255 at 5004.302 kb on - strand, within CA265_RS20255 at 5004.302 kb on - strand, within CA265_RS20255 at 5004.338 kb on + strand, within CA265_RS20255 at 5004.339 kb on - strand, within CA265_RS20255 at 5004.392 kb on + strand, within CA265_RS20255 at 5004.489 kb on + strand, within CA265_RS20255 at 5004.490 kb on - strand, within CA265_RS20255 at 5004.490 kb on - strand, within CA265_RS20255 at 5004.490 kb on - strand, within CA265_RS20255 at 5004.566 kb on - strand, within CA265_RS20255 at 5004.673 kb on - strand, within CA265_RS20255 at 5004.746 kb on + strand, within CA265_RS20255 at 5004.839 kb on - strand, within CA265_RS20255 at 5004.871 kb on - strand at 5004.894 kb on + strand at 5004.895 kb on - strand at 5004.943 kb on - strand at 5004.943 kb on - strand at 5004.987 kb on + strand at 5004.987 kb on + strand at 5005.014 kb on - strand at 5005.055 kb on - strand, within CA265_RS20260 at 5005.094 kb on - strand at 5005.213 kb on + strand, within CA265_RS20265 at 5005.213 kb on + strand, within CA265_RS20265 at 5005.254 kb on + strand, within CA265_RS20265 at 5005.317 kb on + strand at 5005.318 kb on - strand at 5005.322 kb on - strand at 5005.338 kb on - strand at 5005.338 kb on - strand at 5005.341 kb on + strand at 5005.341 kb on + strand at 5005.521 kb on - strand at 5005.521 kb on - strand at 5005.532 kb on - strand at 5005.541 kb on + strand at 5005.572 kb on - strand at 5005.583 kb on + strand at 5005.605 kb on + strand at 5005.648 kb on + strand at 5005.648 kb on + strand at 5005.662 kb on + strand at 5005.703 kb on - strand at 5005.892 kb on - strand, within CA265_RS20270 at 5005.940 kb on - strand, within CA265_RS20270 at 5005.942 kb on - strand, within CA265_RS20270 at 5005.942 kb on - strand, within CA265_RS20270 at 5005.960 kb on - strand, within CA265_RS20270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 5,004,126 + -0.3 5,004,190 + +0.3 5,004,197 + -0.0 5,004,200 - CA265_RS20255 0.10 -0.3 5,004,277 + CA265_RS20255 0.20 +1.0 5,004,277 + CA265_RS20255 0.20 -0.1 5,004,278 - CA265_RS20255 0.20 +0.8 5,004,301 + CA265_RS20255 0.23 +0.3 5,004,301 + CA265_RS20255 0.23 -0.7 5,004,302 - CA265_RS20255 0.23 -0.0 5,004,302 - CA265_RS20255 0.23 -0.7 5,004,338 + CA265_RS20255 0.27 +0.1 5,004,339 - CA265_RS20255 0.27 -1.9 5,004,392 + CA265_RS20255 0.34 +0.1 5,004,489 + CA265_RS20255 0.46 +1.8 5,004,490 - CA265_RS20255 0.46 -0.1 5,004,490 - CA265_RS20255 0.46 -0.7 5,004,490 - CA265_RS20255 0.46 -0.1 5,004,566 - CA265_RS20255 0.56 +0.4 5,004,673 - CA265_RS20255 0.69 -0.2 5,004,746 + CA265_RS20255 0.78 -0.4 5,004,839 - CA265_RS20255 0.89 -1.0 5,004,871 - -0.1 5,004,894 + +0.4 5,004,895 - -0.2 5,004,943 - +0.6 5,004,943 - +1.2 5,004,987 + +0.3 5,004,987 + -0.4 5,005,014 - -0.3 5,005,055 - CA265_RS20260 0.39 +0.4 5,005,094 - -2.3 5,005,213 + CA265_RS20265 0.49 -1.6 5,005,213 + CA265_RS20265 0.49 -0.3 5,005,254 + CA265_RS20265 0.66 -0.2 5,005,317 + -0.1 5,005,318 - -0.2 5,005,322 - +0.4 5,005,338 - -0.8 5,005,338 - -0.6 5,005,341 + +0.3 5,005,341 + -0.4 5,005,521 - +0.6 5,005,521 - +0.2 5,005,532 - -0.8 5,005,541 + -0.3 5,005,572 - +0.4 5,005,583 + +1.4 5,005,605 + -0.2 5,005,648 + -0.0 5,005,648 + -0.3 5,005,662 + -0.1 5,005,703 - +0.1 5,005,892 - CA265_RS20270 0.11 +0.2 5,005,940 - CA265_RS20270 0.13 +1.6 5,005,942 - CA265_RS20270 0.13 -0.6 5,005,942 - CA265_RS20270 0.13 +0.2 5,005,960 - CA265_RS20270 0.14 +1.0
Or see this region's nucleotide sequence