Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15255 and CA265_RS15260 are separated by 55 nucleotides CA265_RS15260 and CA265_RS15265 are separated by 179 nucleotides CA265_RS15265 and CA265_RS15270 are separated by 1 nucleotides CA265_RS15270 and CA265_RS15275 overlap by 1 nucleotides
CA265_RS15255: CA265_RS15255 - restriction endonuclease subunit S, at 3,835,714 to 3,836,670
_RS15255
CA265_RS15260: CA265_RS15260 - hypothetical protein, at 3,836,726 to 3,837,409
_RS15260
CA265_RS15265: CA265_RS15265 - TetR family transcriptional regulator, at 3,837,589 to 3,838,164
_RS15265
CA265_RS15270: CA265_RS15270 - hypothetical protein, at 3,838,166 to 3,838,576
_RS15270
CA265_RS15275: CA265_RS15275 - phenylalanine--tRNA ligase subunit alpha, at 3,838,576 to 3,839,616
_RS15275
Position (kb)
3837
3838
3839 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3836.617 kb on - strand at 3836.752 kb on + strand at 3836.876 kb on - strand, within CA265_RS15260 at 3836.882 kb on - strand, within CA265_RS15260 at 3836.888 kb on + strand, within CA265_RS15260 at 3836.889 kb on - strand, within CA265_RS15260 at 3836.894 kb on - strand, within CA265_RS15260 at 3836.937 kb on + strand, within CA265_RS15260 at 3837.078 kb on - strand, within CA265_RS15260 at 3837.091 kb on + strand, within CA265_RS15260 at 3837.092 kb on + strand, within CA265_RS15260 at 3837.092 kb on + strand, within CA265_RS15260 at 3837.093 kb on - strand, within CA265_RS15260 at 3837.106 kb on - strand, within CA265_RS15260 at 3837.273 kb on + strand, within CA265_RS15260 at 3837.274 kb on - strand, within CA265_RS15260 at 3837.274 kb on - strand, within CA265_RS15260 at 3837.274 kb on - strand, within CA265_RS15260 at 3837.295 kb on + strand, within CA265_RS15260 at 3837.319 kb on + strand, within CA265_RS15260 at 3837.319 kb on + strand, within CA265_RS15260 at 3837.335 kb on - strand, within CA265_RS15260 at 3837.395 kb on + strand at 3837.398 kb on + strand at 3837.398 kb on + strand at 3837.407 kb on + strand at 3837.408 kb on - strand at 3837.408 kb on - strand at 3837.451 kb on - strand at 3837.493 kb on + strand at 3837.498 kb on - strand at 3837.675 kb on + strand, within CA265_RS15265 at 3837.716 kb on - strand, within CA265_RS15265 at 3837.747 kb on + strand, within CA265_RS15265 at 3837.773 kb on + strand, within CA265_RS15265 at 3837.864 kb on + strand, within CA265_RS15265 at 3837.865 kb on - strand, within CA265_RS15265 at 3837.873 kb on + strand, within CA265_RS15265 at 3837.941 kb on + strand, within CA265_RS15265 at 3837.942 kb on - strand, within CA265_RS15265 at 3837.947 kb on - strand, within CA265_RS15265 at 3838.007 kb on - strand, within CA265_RS15265 at 3838.020 kb on + strand, within CA265_RS15265 at 3838.020 kb on + strand, within CA265_RS15265 at 3838.042 kb on - strand, within CA265_RS15265 at 3838.181 kb on + strand at 3838.182 kb on - strand at 3838.185 kb on - strand at 3838.210 kb on + strand, within CA265_RS15270 at 3838.211 kb on - strand, within CA265_RS15270 at 3838.221 kb on - strand, within CA265_RS15270 at 3838.221 kb on - strand, within CA265_RS15270 at 3838.221 kb on - strand, within CA265_RS15270 at 3838.253 kb on - strand, within CA265_RS15270 at 3838.346 kb on + strand, within CA265_RS15270 at 3838.354 kb on + strand, within CA265_RS15270 at 3838.374 kb on + strand, within CA265_RS15270 at 3838.374 kb on + strand, within CA265_RS15270 at 3838.436 kb on + strand, within CA265_RS15270 at 3838.442 kb on - strand, within CA265_RS15270 at 3838.482 kb on + strand, within CA265_RS15270 at 3838.483 kb on - strand, within CA265_RS15270 at 3838.483 kb on - strand, within CA265_RS15270 at 3838.483 kb on - strand, within CA265_RS15270 at 3838.545 kb on - strand at 3838.562 kb on + strand at 3838.563 kb on - strand at 3838.566 kb on + strand at 3838.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 3,836,617 - -2.6 3,836,752 + -0.8 3,836,876 - CA265_RS15260 0.22 -0.0 3,836,882 - CA265_RS15260 0.23 -1.8 3,836,888 + CA265_RS15260 0.24 -0.3 3,836,889 - CA265_RS15260 0.24 +0.2 3,836,894 - CA265_RS15260 0.25 -0.8 3,836,937 + CA265_RS15260 0.31 -0.8 3,837,078 - CA265_RS15260 0.51 +0.2 3,837,091 + CA265_RS15260 0.53 -0.0 3,837,092 + CA265_RS15260 0.54 +0.6 3,837,092 + CA265_RS15260 0.54 -0.7 3,837,093 - CA265_RS15260 0.54 -0.1 3,837,106 - CA265_RS15260 0.56 -0.0 3,837,273 + CA265_RS15260 0.80 +1.0 3,837,274 - CA265_RS15260 0.80 +0.3 3,837,274 - CA265_RS15260 0.80 -0.2 3,837,274 - CA265_RS15260 0.80 +1.5 3,837,295 + CA265_RS15260 0.83 -0.1 3,837,319 + CA265_RS15260 0.87 -0.1 3,837,319 + CA265_RS15260 0.87 -0.4 3,837,335 - CA265_RS15260 0.89 +0.2 3,837,395 + +0.4 3,837,398 + -0.7 3,837,398 + +0.1 3,837,407 + -0.7 3,837,408 - +0.7 3,837,408 - +1.0 3,837,451 - +0.3 3,837,493 + +0.2 3,837,498 - +0.1 3,837,675 + CA265_RS15265 0.15 -1.5 3,837,716 - CA265_RS15265 0.22 -0.9 3,837,747 + CA265_RS15265 0.27 -0.7 3,837,773 + CA265_RS15265 0.32 -2.0 3,837,864 + CA265_RS15265 0.48 -0.8 3,837,865 - CA265_RS15265 0.48 +0.3 3,837,873 + CA265_RS15265 0.49 -1.0 3,837,941 + CA265_RS15265 0.61 +1.1 3,837,942 - CA265_RS15265 0.61 -0.9 3,837,947 - CA265_RS15265 0.62 -0.8 3,838,007 - CA265_RS15265 0.73 -0.3 3,838,020 + CA265_RS15265 0.75 -1.8 3,838,020 + CA265_RS15265 0.75 -0.8 3,838,042 - CA265_RS15265 0.79 -0.8 3,838,181 + -0.9 3,838,182 - +0.6 3,838,185 - +0.0 3,838,210 + CA265_RS15270 0.11 -0.7 3,838,211 - CA265_RS15270 0.11 +0.1 3,838,221 - CA265_RS15270 0.13 +0.1 3,838,221 - CA265_RS15270 0.13 +0.2 3,838,221 - CA265_RS15270 0.13 +2.2 3,838,253 - CA265_RS15270 0.21 +1.3 3,838,346 + CA265_RS15270 0.44 -0.8 3,838,354 + CA265_RS15270 0.46 -0.4 3,838,374 + CA265_RS15270 0.51 -2.0 3,838,374 + CA265_RS15270 0.51 +0.2 3,838,436 + CA265_RS15270 0.66 -0.6 3,838,442 - CA265_RS15270 0.67 -0.2 3,838,482 + CA265_RS15270 0.77 -3.1 3,838,483 - CA265_RS15270 0.77 +0.0 3,838,483 - CA265_RS15270 0.77 +0.3 3,838,483 - CA265_RS15270 0.77 -0.2 3,838,545 - -0.6 3,838,562 + -0.8 3,838,563 - +1.0 3,838,566 + -0.4 3,838,567 - +0.2
Or see this region's nucleotide sequence