Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09510 and CA265_RS09515 are separated by 110 nucleotides CA265_RS09515 and CA265_RS09520 are separated by 46 nucleotides CA265_RS09520 and CA265_RS09525 are separated by 159 nucleotides
CA265_RS09510: CA265_RS09510 - glycosyl transferase family 2, at 2,356,116 to 2,357,129
_RS09510
CA265_RS09515: CA265_RS09515 - type II 3-dehydroquinate dehydratase, at 2,357,240 to 2,357,659
_RS09515
CA265_RS09520: CA265_RS09520 - site-specific tyrosine recombinase XerD, at 2,357,706 to 2,358,605
_RS09520
CA265_RS09525: CA265_RS09525 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 2,358,765 to 2,359,652
_RS09525
Position (kb)
2357
2358
2359 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2357.127 kb on + strand at 2357.181 kb on - strand at 2357.660 kb on - strand at 2357.661 kb on + strand at 2357.662 kb on - strand at 2357.663 kb on - strand at 2357.669 kb on + strand at 2357.721 kb on + strand at 2357.723 kb on + strand at 2357.804 kb on + strand, within CA265_RS09520 at 2357.856 kb on - strand, within CA265_RS09520 at 2357.857 kb on + strand, within CA265_RS09520 at 2357.873 kb on - strand, within CA265_RS09520 at 2357.882 kb on + strand, within CA265_RS09520 at 2357.944 kb on + strand, within CA265_RS09520 at 2357.961 kb on + strand, within CA265_RS09520 at 2357.965 kb on + strand, within CA265_RS09520 at 2357.966 kb on - strand, within CA265_RS09520 at 2358.082 kb on + strand, within CA265_RS09520 at 2358.083 kb on - strand, within CA265_RS09520 at 2358.083 kb on - strand, within CA265_RS09520 at 2358.130 kb on + strand, within CA265_RS09520 at 2358.135 kb on + strand, within CA265_RS09520 at 2358.301 kb on + strand, within CA265_RS09520 at 2358.302 kb on - strand, within CA265_RS09520 at 2358.308 kb on + strand, within CA265_RS09520 at 2358.308 kb on + strand, within CA265_RS09520 at 2358.371 kb on + strand, within CA265_RS09520 at 2358.474 kb on - strand, within CA265_RS09520 at 2358.500 kb on - strand, within CA265_RS09520 at 2358.528 kb on - strand at 2358.528 kb on - strand at 2358.528 kb on - strand at 2358.530 kb on + strand at 2358.545 kb on + strand at 2358.564 kb on + strand at 2358.604 kb on - strand at 2358.610 kb on - strand at 2358.652 kb on - strand at 2358.652 kb on - strand at 2358.695 kb on - strand at 2358.709 kb on + strand at 2358.852 kb on + strand at 2358.916 kb on + strand, within CA265_RS09525 at 2358.942 kb on - strand, within CA265_RS09525 at 2358.963 kb on - strand, within CA265_RS09525 at 2358.963 kb on - strand, within CA265_RS09525 at 2358.969 kb on - strand, within CA265_RS09525 at 2358.969 kb on - strand, within CA265_RS09525 at 2358.976 kb on - strand, within CA265_RS09525 at 2359.043 kb on + strand, within CA265_RS09525 at 2359.079 kb on + strand, within CA265_RS09525 at 2359.091 kb on + strand, within CA265_RS09525 at 2359.179 kb on + strand, within CA265_RS09525 at 2359.215 kb on + strand, within CA265_RS09525 at 2359.230 kb on + strand, within CA265_RS09525 at 2359.241 kb on + strand, within CA265_RS09525 at 2359.376 kb on + strand, within CA265_RS09525 at 2359.442 kb on + strand, within CA265_RS09525 at 2359.548 kb on + strand, within CA265_RS09525 at 2359.600 kb on + strand at 2359.601 kb on - strand at 2359.602 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 2,357,127 + +0.0 2,357,181 - +1.3 2,357,660 - -1.4 2,357,661 + +0.0 2,357,662 - -0.3 2,357,663 - -0.6 2,357,669 + -0.4 2,357,721 + -1.0 2,357,723 + -3.4 2,357,804 + CA265_RS09520 0.11 -1.5 2,357,856 - CA265_RS09520 0.17 -0.9 2,357,857 + CA265_RS09520 0.17 -0.5 2,357,873 - CA265_RS09520 0.19 -1.9 2,357,882 + CA265_RS09520 0.20 -1.7 2,357,944 + CA265_RS09520 0.26 -2.4 2,357,961 + CA265_RS09520 0.28 -3.3 2,357,965 + CA265_RS09520 0.29 -1.3 2,357,966 - CA265_RS09520 0.29 -1.0 2,358,082 + CA265_RS09520 0.42 -2.6 2,358,083 - CA265_RS09520 0.42 -0.0 2,358,083 - CA265_RS09520 0.42 -0.7 2,358,130 + CA265_RS09520 0.47 -0.7 2,358,135 + CA265_RS09520 0.48 -1.6 2,358,301 + CA265_RS09520 0.66 -2.1 2,358,302 - CA265_RS09520 0.66 -0.5 2,358,308 + CA265_RS09520 0.67 -0.3 2,358,308 + CA265_RS09520 0.67 -1.3 2,358,371 + CA265_RS09520 0.74 -1.1 2,358,474 - CA265_RS09520 0.85 -1.0 2,358,500 - CA265_RS09520 0.88 -2.7 2,358,528 - -2.1 2,358,528 - -2.0 2,358,528 - -0.3 2,358,530 + -4.1 2,358,545 + -1.8 2,358,564 + -1.8 2,358,604 - +0.5 2,358,610 - -0.0 2,358,652 - -0.8 2,358,652 - -0.7 2,358,695 - -1.0 2,358,709 + -0.4 2,358,852 + -2.6 2,358,916 + CA265_RS09525 0.17 -0.8 2,358,942 - CA265_RS09525 0.20 -1.4 2,358,963 - CA265_RS09525 0.22 -1.5 2,358,963 - CA265_RS09525 0.22 -2.1 2,358,969 - CA265_RS09525 0.23 -2.2 2,358,969 - CA265_RS09525 0.23 -2.3 2,358,976 - CA265_RS09525 0.24 -2.9 2,359,043 + CA265_RS09525 0.31 -3.0 2,359,079 + CA265_RS09525 0.35 -1.8 2,359,091 + CA265_RS09525 0.37 -1.3 2,359,179 + CA265_RS09525 0.47 -3.5 2,359,215 + CA265_RS09525 0.51 -1.2 2,359,230 + CA265_RS09525 0.52 -3.1 2,359,241 + CA265_RS09525 0.54 -1.5 2,359,376 + CA265_RS09525 0.69 -0.7 2,359,442 + CA265_RS09525 0.76 -1.7 2,359,548 + CA265_RS09525 0.88 -2.1 2,359,600 + -1.2 2,359,601 - +0.3 2,359,602 + -2.3
Or see this region's nucleotide sequence