Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05035 and CA265_RS05040 are separated by 70 nucleotides CA265_RS05040 and CA265_RS05045 are separated by 24 nucleotides
CA265_RS05035: CA265_RS05035 - TetR family transcriptional regulator, at 1,195,205 to 1,195,810
_RS05035
CA265_RS05040: CA265_RS05040 - efflux transporter periplasmic adaptor subunit, at 1,195,881 to 1,197,083
_RS05040
CA265_RS05045: CA265_RS05045 - hydrophobe/amphiphile efflux-1 family RND transporter, at 1,197,108 to 1,200,308
_RS05045
Position (kb)
1195
1196
1197
1198 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1194.952 kb on - strand at 1195.052 kb on + strand at 1195.091 kb on - strand at 1195.316 kb on - strand, within CA265_RS05035 at 1195.337 kb on + strand, within CA265_RS05035 at 1195.410 kb on - strand, within CA265_RS05035 at 1195.413 kb on + strand, within CA265_RS05035 at 1195.430 kb on - strand, within CA265_RS05035 at 1195.447 kb on + strand, within CA265_RS05035 at 1195.468 kb on + strand, within CA265_RS05035 at 1195.539 kb on - strand, within CA265_RS05035 at 1195.539 kb on - strand, within CA265_RS05035 at 1195.550 kb on - strand, within CA265_RS05035 at 1195.550 kb on - strand, within CA265_RS05035 at 1195.562 kb on - strand, within CA265_RS05035 at 1195.604 kb on - strand, within CA265_RS05035 at 1195.618 kb on - strand, within CA265_RS05035 at 1195.618 kb on - strand, within CA265_RS05035 at 1195.660 kb on + strand, within CA265_RS05035 at 1195.674 kb on - strand, within CA265_RS05035 at 1195.720 kb on - strand, within CA265_RS05035 at 1195.799 kb on - strand at 1195.841 kb on - strand at 1195.856 kb on + strand at 1195.856 kb on + strand at 1195.880 kb on + strand at 1195.881 kb on - strand at 1195.966 kb on + strand at 1195.966 kb on + strand at 1195.967 kb on - strand at 1195.967 kb on - strand at 1195.972 kb on + strand at 1195.972 kb on + strand at 1195.989 kb on - strand at 1195.989 kb on - strand at 1196.041 kb on + strand, within CA265_RS05040 at 1196.074 kb on - strand, within CA265_RS05040 at 1196.144 kb on - strand, within CA265_RS05040 at 1196.152 kb on + strand, within CA265_RS05040 at 1196.173 kb on - strand, within CA265_RS05040 at 1196.275 kb on + strand, within CA265_RS05040 at 1196.298 kb on - strand, within CA265_RS05040 at 1196.397 kb on - strand, within CA265_RS05040 at 1196.444 kb on + strand, within CA265_RS05040 at 1196.497 kb on + strand, within CA265_RS05040 at 1196.559 kb on + strand, within CA265_RS05040 at 1196.613 kb on - strand, within CA265_RS05040 at 1196.641 kb on + strand, within CA265_RS05040 at 1196.702 kb on + strand, within CA265_RS05040 at 1196.789 kb on + strand, within CA265_RS05040 at 1196.871 kb on - strand, within CA265_RS05040 at 1196.875 kb on + strand, within CA265_RS05040 at 1196.875 kb on + strand, within CA265_RS05040 at 1196.876 kb on - strand, within CA265_RS05040 at 1196.876 kb on - strand, within CA265_RS05040 at 1196.890 kb on + strand, within CA265_RS05040 at 1196.904 kb on - strand, within CA265_RS05040 at 1196.996 kb on - strand at 1197.013 kb on + strand at 1197.014 kb on - strand at 1197.150 kb on - strand at 1197.169 kb on + strand at 1197.169 kb on + strand at 1197.170 kb on - strand at 1197.252 kb on + strand at 1197.280 kb on + strand at 1197.336 kb on + strand at 1197.337 kb on - strand at 1197.337 kb on - strand at 1197.337 kb on - strand at 1197.365 kb on + strand at 1197.398 kb on + strand at 1197.398 kb on + strand at 1197.422 kb on + strand at 1197.422 kb on + strand at 1197.423 kb on - strand at 1197.473 kb on - strand, within CA265_RS05045 at 1197.476 kb on - strand, within CA265_RS05045 at 1197.476 kb on - strand, within CA265_RS05045 at 1197.481 kb on - strand, within CA265_RS05045 at 1197.576 kb on - strand, within CA265_RS05045 at 1197.593 kb on + strand, within CA265_RS05045 at 1197.593 kb on + strand, within CA265_RS05045 at 1197.609 kb on - strand, within CA265_RS05045 at 1197.614 kb on + strand, within CA265_RS05045 at 1197.641 kb on - strand, within CA265_RS05045 at 1197.711 kb on - strand, within CA265_RS05045 at 1197.716 kb on + strand, within CA265_RS05045 at 1197.716 kb on + strand, within CA265_RS05045 at 1197.717 kb on - strand, within CA265_RS05045 at 1197.809 kb on + strand, within CA265_RS05045 at 1197.809 kb on + strand, within CA265_RS05045 at 1197.810 kb on - strand, within CA265_RS05045 at 1197.813 kb on + strand, within CA265_RS05045 at 1197.815 kb on + strand, within CA265_RS05045 at 1197.821 kb on + strand, within CA265_RS05045 at 1197.882 kb on - strand, within CA265_RS05045 at 1197.887 kb on - strand, within CA265_RS05045 at 1197.931 kb on - strand, within CA265_RS05045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 1,194,952 - +0.7 1,195,052 + +4.0 1,195,091 - +0.6 1,195,316 - CA265_RS05035 0.18 -1.1 1,195,337 + CA265_RS05035 0.22 +2.7 1,195,410 - CA265_RS05035 0.34 -1.5 1,195,413 + CA265_RS05035 0.34 +3.3 1,195,430 - CA265_RS05035 0.37 +1.6 1,195,447 + CA265_RS05035 0.40 +1.9 1,195,468 + CA265_RS05035 0.43 +2.4 1,195,539 - CA265_RS05035 0.55 -0.1 1,195,539 - CA265_RS05035 0.55 -0.0 1,195,550 - CA265_RS05035 0.57 -2.2 1,195,550 - CA265_RS05035 0.57 -0.2 1,195,562 - CA265_RS05035 0.59 +1.9 1,195,604 - CA265_RS05035 0.66 -0.5 1,195,618 - CA265_RS05035 0.68 +1.1 1,195,618 - CA265_RS05035 0.68 +0.9 1,195,660 + CA265_RS05035 0.75 +2.7 1,195,674 - CA265_RS05035 0.77 +0.8 1,195,720 - CA265_RS05035 0.85 +0.6 1,195,799 - +2.4 1,195,841 - -1.6 1,195,856 + +5.5 1,195,856 + +5.1 1,195,880 + -2.2 1,195,881 - -1.7 1,195,966 + -1.9 1,195,966 + -1.3 1,195,967 - -1.7 1,195,967 - -1.5 1,195,972 + -2.0 1,195,972 + -2.6 1,195,989 - -1.3 1,195,989 - +0.9 1,196,041 + CA265_RS05040 0.13 -1.5 1,196,074 - CA265_RS05040 0.16 -3.6 1,196,144 - CA265_RS05040 0.22 -2.3 1,196,152 + CA265_RS05040 0.23 -1.1 1,196,173 - CA265_RS05040 0.24 -1.2 1,196,275 + CA265_RS05040 0.33 +0.5 1,196,298 - CA265_RS05040 0.35 -2.0 1,196,397 - CA265_RS05040 0.43 -2.8 1,196,444 + CA265_RS05040 0.47 -1.9 1,196,497 + CA265_RS05040 0.51 -1.9 1,196,559 + CA265_RS05040 0.56 -2.7 1,196,613 - CA265_RS05040 0.61 -2.1 1,196,641 + CA265_RS05040 0.63 -1.2 1,196,702 + CA265_RS05040 0.68 -2.0 1,196,789 + CA265_RS05040 0.75 -1.5 1,196,871 - CA265_RS05040 0.82 -1.2 1,196,875 + CA265_RS05040 0.83 +0.3 1,196,875 + CA265_RS05040 0.83 -1.8 1,196,876 - CA265_RS05040 0.83 -2.2 1,196,876 - CA265_RS05040 0.83 -1.7 1,196,890 + CA265_RS05040 0.84 -1.1 1,196,904 - CA265_RS05040 0.85 -1.2 1,196,996 - -2.0 1,197,013 + -2.5 1,197,014 - -0.1 1,197,150 - -1.2 1,197,169 + -1.8 1,197,169 + -3.1 1,197,170 - -1.0 1,197,252 + -2.6 1,197,280 + -2.3 1,197,336 + -2.3 1,197,337 - -1.6 1,197,337 - -2.0 1,197,337 - -2.3 1,197,365 + -2.2 1,197,398 + -1.2 1,197,398 + -3.4 1,197,422 + -1.4 1,197,422 + -1.3 1,197,423 - -1.5 1,197,473 - CA265_RS05045 0.11 -2.4 1,197,476 - CA265_RS05045 0.11 -2.2 1,197,476 - CA265_RS05045 0.11 -2.1 1,197,481 - CA265_RS05045 0.12 -2.0 1,197,576 - CA265_RS05045 0.15 -2.9 1,197,593 + CA265_RS05045 0.15 -2.0 1,197,593 + CA265_RS05045 0.15 -2.6 1,197,609 - CA265_RS05045 0.16 -2.8 1,197,614 + CA265_RS05045 0.16 -2.3 1,197,641 - CA265_RS05045 0.17 -1.8 1,197,711 - CA265_RS05045 0.19 -3.0 1,197,716 + CA265_RS05045 0.19 -1.3 1,197,716 + CA265_RS05045 0.19 -2.5 1,197,717 - CA265_RS05045 0.19 -0.6 1,197,809 + CA265_RS05045 0.22 -1.1 1,197,809 + CA265_RS05045 0.22 -2.1 1,197,810 - CA265_RS05045 0.22 -3.8 1,197,813 + CA265_RS05045 0.22 -2.5 1,197,815 + CA265_RS05045 0.22 -0.8 1,197,821 + CA265_RS05045 0.22 -4.2 1,197,882 - CA265_RS05045 0.24 -2.3 1,197,887 - CA265_RS05045 0.24 -2.2 1,197,931 - CA265_RS05045 0.26 -1.4
Or see this region's nucleotide sequence