Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05030 and CA265_RS05035 are separated by 571 nucleotides CA265_RS05035 and CA265_RS05040 are separated by 70 nucleotides
CA265_RS05030: CA265_RS05030 - GNAT family N-acetyltransferase, at 1,194,190 to 1,194,633
_RS05030
CA265_RS05035: CA265_RS05035 - TetR family transcriptional regulator, at 1,195,205 to 1,195,810
_RS05035
CA265_RS05040: CA265_RS05040 - efflux transporter periplasmic adaptor subunit, at 1,195,881 to 1,197,083
_RS05040
Position (kb)
1195
1196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1194.283 kb on - strand, within CA265_RS05030 at 1194.351 kb on - strand, within CA265_RS05030 at 1194.432 kb on + strand, within CA265_RS05030 at 1194.432 kb on + strand, within CA265_RS05030 at 1194.432 kb on + strand, within CA265_RS05030 at 1194.433 kb on - strand, within CA265_RS05030 at 1194.433 kb on - strand, within CA265_RS05030 at 1194.433 kb on - strand, within CA265_RS05030 at 1194.645 kb on - strand at 1194.748 kb on + strand at 1194.855 kb on - strand at 1194.858 kb on + strand at 1194.952 kb on - strand at 1195.052 kb on + strand at 1195.091 kb on - strand at 1195.316 kb on - strand, within CA265_RS05035 at 1195.337 kb on + strand, within CA265_RS05035 at 1195.410 kb on - strand, within CA265_RS05035 at 1195.413 kb on + strand, within CA265_RS05035 at 1195.430 kb on - strand, within CA265_RS05035 at 1195.447 kb on + strand, within CA265_RS05035 at 1195.468 kb on + strand, within CA265_RS05035 at 1195.539 kb on - strand, within CA265_RS05035 at 1195.539 kb on - strand, within CA265_RS05035 at 1195.550 kb on - strand, within CA265_RS05035 at 1195.550 kb on - strand, within CA265_RS05035 at 1195.562 kb on - strand, within CA265_RS05035 at 1195.604 kb on - strand, within CA265_RS05035 at 1195.618 kb on - strand, within CA265_RS05035 at 1195.618 kb on - strand, within CA265_RS05035 at 1195.660 kb on + strand, within CA265_RS05035 at 1195.674 kb on - strand, within CA265_RS05035 at 1195.720 kb on - strand, within CA265_RS05035 at 1195.799 kb on - strand at 1195.841 kb on - strand at 1195.856 kb on + strand at 1195.856 kb on + strand at 1195.880 kb on + strand at 1195.881 kb on - strand at 1195.966 kb on + strand at 1195.966 kb on + strand at 1195.967 kb on - strand at 1195.967 kb on - strand at 1195.972 kb on + strand at 1195.972 kb on + strand at 1195.989 kb on - strand at 1195.989 kb on - strand at 1196.041 kb on + strand, within CA265_RS05040 at 1196.074 kb on - strand, within CA265_RS05040 at 1196.144 kb on - strand, within CA265_RS05040 at 1196.152 kb on + strand, within CA265_RS05040 at 1196.173 kb on - strand, within CA265_RS05040 at 1196.275 kb on + strand, within CA265_RS05040 at 1196.298 kb on - strand, within CA265_RS05040 at 1196.397 kb on - strand, within CA265_RS05040 at 1196.444 kb on + strand, within CA265_RS05040 at 1196.497 kb on + strand, within CA265_RS05040 at 1196.559 kb on + strand, within CA265_RS05040 at 1196.613 kb on - strand, within CA265_RS05040 at 1196.641 kb on + strand, within CA265_RS05040 at 1196.702 kb on + strand, within CA265_RS05040 at 1196.789 kb on + strand, within CA265_RS05040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 1,194,283 - CA265_RS05030 0.21 +1.0 1,194,351 - CA265_RS05030 0.36 +0.1 1,194,432 + CA265_RS05030 0.55 +1.9 1,194,432 + CA265_RS05030 0.55 +1.5 1,194,432 + CA265_RS05030 0.55 +2.2 1,194,433 - CA265_RS05030 0.55 +1.0 1,194,433 - CA265_RS05030 0.55 +0.3 1,194,433 - CA265_RS05030 0.55 +0.6 1,194,645 - -0.1 1,194,748 + +5.0 1,194,855 - +0.7 1,194,858 + +4.6 1,194,952 - +0.7 1,195,052 + +4.0 1,195,091 - +0.6 1,195,316 - CA265_RS05035 0.18 -1.1 1,195,337 + CA265_RS05035 0.22 +2.7 1,195,410 - CA265_RS05035 0.34 -1.5 1,195,413 + CA265_RS05035 0.34 +3.3 1,195,430 - CA265_RS05035 0.37 +1.6 1,195,447 + CA265_RS05035 0.40 +1.9 1,195,468 + CA265_RS05035 0.43 +2.4 1,195,539 - CA265_RS05035 0.55 -0.1 1,195,539 - CA265_RS05035 0.55 -0.0 1,195,550 - CA265_RS05035 0.57 -2.2 1,195,550 - CA265_RS05035 0.57 -0.2 1,195,562 - CA265_RS05035 0.59 +1.9 1,195,604 - CA265_RS05035 0.66 -0.5 1,195,618 - CA265_RS05035 0.68 +1.1 1,195,618 - CA265_RS05035 0.68 +0.9 1,195,660 + CA265_RS05035 0.75 +2.7 1,195,674 - CA265_RS05035 0.77 +0.8 1,195,720 - CA265_RS05035 0.85 +0.6 1,195,799 - +2.4 1,195,841 - -1.6 1,195,856 + +5.5 1,195,856 + +5.1 1,195,880 + -2.2 1,195,881 - -1.7 1,195,966 + -1.9 1,195,966 + -1.3 1,195,967 - -1.7 1,195,967 - -1.5 1,195,972 + -2.0 1,195,972 + -2.6 1,195,989 - -1.3 1,195,989 - +0.9 1,196,041 + CA265_RS05040 0.13 -1.5 1,196,074 - CA265_RS05040 0.16 -3.6 1,196,144 - CA265_RS05040 0.22 -2.3 1,196,152 + CA265_RS05040 0.23 -1.1 1,196,173 - CA265_RS05040 0.24 -1.2 1,196,275 + CA265_RS05040 0.33 +0.5 1,196,298 - CA265_RS05040 0.35 -2.0 1,196,397 - CA265_RS05040 0.43 -2.8 1,196,444 + CA265_RS05040 0.47 -1.9 1,196,497 + CA265_RS05040 0.51 -1.9 1,196,559 + CA265_RS05040 0.56 -2.7 1,196,613 - CA265_RS05040 0.61 -2.1 1,196,641 + CA265_RS05040 0.63 -1.2 1,196,702 + CA265_RS05040 0.68 -2.0 1,196,789 + CA265_RS05040 0.75 -1.5
Or see this region's nucleotide sequence