Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05005 and CA265_RS05010 are separated by 286 nucleotides CA265_RS05010 and CA265_RS05015 overlap by 13 nucleotides CA265_RS05015 and CA265_RS05020 are separated by 51 nucleotides
CA265_RS05005: CA265_RS05005 - ion transporter, at 1,188,856 to 1,189,797
_RS05005
CA265_RS05010: CA265_RS05010 - anthranilate phosphoribosyltransferase (EC 2.4.2.18) <small>(from data)</small>, at 1,190,084 to 1,191,073
_RS05010
CA265_RS05015: CA265_RS05015 - N-(5'-phosphoribosyl)anthranilate isomerase, at 1,191,061 to 1,191,696
_RS05015
CA265_RS05020: CA265_RS05020 - tryptophan synthase subunit beta, at 1,191,748 to 1,192,929
_RS05020
Position (kb) 1190
1191
1192 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1189.105 kb on + strand, within CA265_RS05005 at 1189.105 kb on + strand, within CA265_RS05005 at 1189.105 kb on + strand, within CA265_RS05005 at 1189.106 kb on - strand, within CA265_RS05005 at 1189.211 kb on - strand, within CA265_RS05005 at 1189.322 kb on - strand, within CA265_RS05005 at 1189.332 kb on + strand, within CA265_RS05005 at 1189.332 kb on + strand, within CA265_RS05005 at 1189.333 kb on - strand, within CA265_RS05005 at 1189.353 kb on - strand, within CA265_RS05005 at 1189.451 kb on + strand, within CA265_RS05005 at 1189.560 kb on - strand, within CA265_RS05005 at 1189.560 kb on - strand, within CA265_RS05005 at 1189.565 kb on - strand, within CA265_RS05005 at 1189.573 kb on + strand, within CA265_RS05005 at 1189.593 kb on - strand, within CA265_RS05005 at 1189.650 kb on - strand, within CA265_RS05005 at 1189.650 kb on - strand, within CA265_RS05005 at 1189.650 kb on - strand, within CA265_RS05005 at 1189.693 kb on - strand, within CA265_RS05005 at 1189.768 kb on - strand at 1189.799 kb on + strand at 1189.849 kb on + strand at 1189.874 kb on + strand at 1190.020 kb on - strand at 1190.032 kb on - strand at 1190.228 kb on - strand, within CA265_RS05010 at 1190.233 kb on - strand, within CA265_RS05010 at 1190.410 kb on + strand, within CA265_RS05010 at 1190.444 kb on - strand, within CA265_RS05010 at 1190.460 kb on + strand, within CA265_RS05010 at 1190.493 kb on + strand, within CA265_RS05010 at 1190.505 kb on + strand, within CA265_RS05010 at 1190.529 kb on + strand, within CA265_RS05010 at 1190.656 kb on - strand, within CA265_RS05010 at 1190.685 kb on + strand, within CA265_RS05010 at 1190.730 kb on + strand, within CA265_RS05010 at 1190.730 kb on + strand, within CA265_RS05010 at 1190.925 kb on + strand, within CA265_RS05010 at 1191.060 kb on + strand at 1191.308 kb on + strand, within CA265_RS05015 at 1191.331 kb on + strand, within CA265_RS05015 at 1191.389 kb on + strand, within CA265_RS05015 at 1191.520 kb on - strand, within CA265_RS05015 at 1191.666 kb on + strand at 1191.694 kb on + strand at 1191.702 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 1,189,105 + CA265_RS05005 0.26 +0.4 1,189,105 + CA265_RS05005 0.26 +0.0 1,189,105 + CA265_RS05005 0.26 -0.1 1,189,106 - CA265_RS05005 0.27 -0.2 1,189,211 - CA265_RS05005 0.38 +0.3 1,189,322 - CA265_RS05005 0.49 -0.4 1,189,332 + CA265_RS05005 0.51 -1.1 1,189,332 + CA265_RS05005 0.51 -0.4 1,189,333 - CA265_RS05005 0.51 -0.1 1,189,353 - CA265_RS05005 0.53 -0.9 1,189,451 + CA265_RS05005 0.63 -0.3 1,189,560 - CA265_RS05005 0.75 -0.9 1,189,560 - CA265_RS05005 0.75 -1.1 1,189,565 - CA265_RS05005 0.75 -0.7 1,189,573 + CA265_RS05005 0.76 -0.5 1,189,593 - CA265_RS05005 0.78 -0.0 1,189,650 - CA265_RS05005 0.84 +0.3 1,189,650 - CA265_RS05005 0.84 -0.1 1,189,650 - CA265_RS05005 0.84 -0.2 1,189,693 - CA265_RS05005 0.89 +0.0 1,189,768 - -0.2 1,189,799 + +0.6 1,189,849 + +0.9 1,189,874 + +0.5 1,190,020 - +0.5 1,190,032 - -0.6 1,190,228 - CA265_RS05010 0.15 +0.3 1,190,233 - CA265_RS05010 0.15 +0.5 1,190,410 + CA265_RS05010 0.33 -1.8 1,190,444 - CA265_RS05010 0.36 -0.1 1,190,460 + CA265_RS05010 0.38 +1.9 1,190,493 + CA265_RS05010 0.41 +0.5 1,190,505 + CA265_RS05010 0.43 -1.0 1,190,529 + CA265_RS05010 0.45 -1.1 1,190,656 - CA265_RS05010 0.58 -1.1 1,190,685 + CA265_RS05010 0.61 -0.4 1,190,730 + CA265_RS05010 0.65 -1.9 1,190,730 + CA265_RS05010 0.65 +1.5 1,190,925 + CA265_RS05010 0.85 -0.1 1,191,060 + +1.5 1,191,308 + CA265_RS05015 0.39 -0.1 1,191,331 + CA265_RS05015 0.42 +1.2 1,191,389 + CA265_RS05015 0.52 -1.8 1,191,520 - CA265_RS05015 0.72 -0.1 1,191,666 + +0.2 1,191,694 + -0.5 1,191,702 + -1.7
Or see this region's nucleotide sequence