Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00775 and CA265_RS00780 are separated by 60 nucleotides CA265_RS00780 and CA265_RS00785 are separated by 295 nucleotides CA265_RS00785 and CA265_RS00790 are separated by 310 nucleotides
CA265_RS00775: CA265_RS00775 - carbonic anhydrase, at 186,125 to 186,751
_RS00775
CA265_RS00780: CA265_RS00780 - hypothetical protein, at 186,812 to 187,321
_RS00780
CA265_RS00785: CA265_RS00785 - hypothetical protein, at 187,617 to 188,228
_RS00785
CA265_RS00790: CA265_RS00790 - Crp/Fnr family transcriptional regulator, at 188,539 to 189,180
_RS00790
Position (kb) 187
188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 186.623 kb on - strand, within CA265_RS00775 at 186.639 kb on + strand, within CA265_RS00775 at 186.691 kb on + strand at 186.743 kb on + strand at 186.743 kb on + strand at 186.783 kb on + strand at 186.821 kb on + strand at 186.823 kb on + strand at 186.891 kb on + strand, within CA265_RS00780 at 187.093 kb on - strand, within CA265_RS00780 at 187.117 kb on + strand, within CA265_RS00780 at 187.118 kb on - strand, within CA265_RS00780 at 187.126 kb on + strand, within CA265_RS00780 at 187.127 kb on - strand, within CA265_RS00780 at 187.131 kb on + strand, within CA265_RS00780 at 187.132 kb on - strand, within CA265_RS00780 at 187.308 kb on - strand at 187.340 kb on - strand at 187.340 kb on - strand at 187.360 kb on + strand at 187.360 kb on + strand at 187.360 kb on + strand at 187.424 kb on + strand at 187.425 kb on - strand at 187.425 kb on - strand at 187.430 kb on - strand at 187.453 kb on - strand at 187.576 kb on + strand at 187.577 kb on - strand at 187.673 kb on + strand at 187.673 kb on + strand at 187.676 kb on - strand at 187.717 kb on + strand, within CA265_RS00785 at 187.718 kb on - strand, within CA265_RS00785 at 187.718 kb on - strand, within CA265_RS00785 at 187.718 kb on - strand, within CA265_RS00785 at 187.759 kb on - strand, within CA265_RS00785 at 187.766 kb on + strand, within CA265_RS00785 at 187.855 kb on - strand, within CA265_RS00785 at 187.987 kb on + strand, within CA265_RS00785 at 188.129 kb on + strand, within CA265_RS00785 at 188.206 kb on + strand at 188.232 kb on + strand at 188.256 kb on + strand at 188.256 kb on + strand at 188.257 kb on - strand at 188.295 kb on - strand at 188.299 kb on - strand at 188.323 kb on - strand at 188.392 kb on - strand at 188.393 kb on + strand at 188.394 kb on - strand at 188.394 kb on - strand at 188.395 kb on + strand at 188.396 kb on - strand at 188.519 kb on + strand at 188.520 kb on - strand at 188.530 kb on + strand at 188.585 kb on - strand at 188.585 kb on - strand at 188.592 kb on + strand at 188.592 kb on + strand at 188.620 kb on - strand, within CA265_RS00790 at 188.750 kb on - strand, within CA265_RS00790 at 188.750 kb on - strand, within CA265_RS00790 at 188.754 kb on + strand, within CA265_RS00790 at 188.756 kb on + strand, within CA265_RS00790 at 188.756 kb on + strand, within CA265_RS00790 at 188.756 kb on + strand, within CA265_RS00790 at 188.757 kb on - strand, within CA265_RS00790 at 188.758 kb on + strand, within CA265_RS00790 at 188.759 kb on - strand, within CA265_RS00790 at 188.791 kb on + strand, within CA265_RS00790 at 188.845 kb on - strand, within CA265_RS00790 at 188.877 kb on + strand, within CA265_RS00790 at 188.899 kb on + strand, within CA265_RS00790 at 188.913 kb on + strand, within CA265_RS00790 at 188.914 kb on - strand, within CA265_RS00790 at 188.914 kb on - strand, within CA265_RS00790 at 188.936 kb on - strand, within CA265_RS00790 at 188.967 kb on + strand, within CA265_RS00790 at 188.968 kb on - strand, within CA265_RS00790 at 189.180 kb on + strand at 189.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 186,623 - CA265_RS00775 0.79 -0.9 186,639 + CA265_RS00775 0.82 -0.0 186,691 + +0.1 186,743 + -0.2 186,743 + +0.6 186,783 + +1.0 186,821 + +0.1 186,823 + -0.5 186,891 + CA265_RS00780 0.15 -0.5 187,093 - CA265_RS00780 0.55 +0.0 187,117 + CA265_RS00780 0.60 -0.7 187,118 - CA265_RS00780 0.60 -0.4 187,126 + CA265_RS00780 0.62 -1.2 187,127 - CA265_RS00780 0.62 -2.0 187,131 + CA265_RS00780 0.63 +0.6 187,132 - CA265_RS00780 0.63 -1.5 187,308 - -0.5 187,340 - -0.2 187,340 - +0.1 187,360 + -0.1 187,360 + -0.4 187,360 + +0.7 187,424 + -1.6 187,425 - -0.3 187,425 - +0.3 187,430 - -0.4 187,453 - -0.6 187,576 + -1.1 187,577 - -0.2 187,673 + -2.1 187,673 + -1.4 187,676 - -0.5 187,717 + CA265_RS00785 0.16 -1.2 187,718 - CA265_RS00785 0.17 -1.6 187,718 - CA265_RS00785 0.17 -2.6 187,718 - CA265_RS00785 0.17 -2.4 187,759 - CA265_RS00785 0.23 -2.9 187,766 + CA265_RS00785 0.24 -2.2 187,855 - CA265_RS00785 0.39 -1.6 187,987 + CA265_RS00785 0.60 -2.7 188,129 + CA265_RS00785 0.84 -2.7 188,206 + -2.5 188,232 + -2.0 188,256 + -3.0 188,256 + -2.4 188,257 - -0.7 188,295 - +0.4 188,299 - +1.2 188,323 - +0.7 188,392 - -0.9 188,393 + -1.7 188,394 - -0.1 188,394 - -0.2 188,395 + -3.0 188,396 - +1.0 188,519 + -0.8 188,520 - -1.1 188,530 + +1.1 188,585 - +0.0 188,585 - +0.9 188,592 + +0.5 188,592 + -0.9 188,620 - CA265_RS00790 0.13 +0.1 188,750 - CA265_RS00790 0.33 +1.4 188,750 - CA265_RS00790 0.33 -1.7 188,754 + CA265_RS00790 0.33 -0.3 188,756 + CA265_RS00790 0.34 -0.0 188,756 + CA265_RS00790 0.34 -0.2 188,756 + CA265_RS00790 0.34 -0.2 188,757 - CA265_RS00790 0.34 +0.6 188,758 + CA265_RS00790 0.34 -0.2 188,759 - CA265_RS00790 0.34 -0.2 188,791 + CA265_RS00790 0.39 -0.7 188,845 - CA265_RS00790 0.48 -0.4 188,877 + CA265_RS00790 0.53 +0.2 188,899 + CA265_RS00790 0.56 -1.6 188,913 + CA265_RS00790 0.58 -0.2 188,914 - CA265_RS00790 0.58 -0.2 188,914 - CA265_RS00790 0.58 +0.1 188,936 - CA265_RS00790 0.62 +0.1 188,967 + CA265_RS00790 0.67 +0.0 188,968 - CA265_RS00790 0.67 +0.1 189,180 + +0.9 189,181 - -1.1
Or see this region's nucleotide sequence