Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00590 and CA265_RS00595 are separated by 223 nucleotides CA265_RS00595 and CA265_RS00600 are separated by 114 nucleotides CA265_RS00600 and CA265_RS00605 are separated by 135 nucleotides
CA265_RS00590: CA265_RS00590 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 134,934 to 135,935
_RS00590
CA265_RS00595: CA265_RS00595 - hypothetical protein, at 136,159 to 136,356
_RS00595
CA265_RS00600: CA265_RS00600 - recombinase RecA, at 136,471 to 137,484
_RS00600
CA265_RS00605: CA265_RS00605 - endonuclease III, at 137,620 to 138,285
_RS00605
Position (kb) 136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 135.934 kb on - strand at 136.148 kb on + strand at 136.148 kb on + strand at 136.158 kb on + strand at 136.158 kb on + strand at 136.158 kb on + strand at 136.159 kb on - strand at 136.308 kb on + strand, within CA265_RS00595 at 136.309 kb on - strand, within CA265_RS00595 at 136.310 kb on + strand, within CA265_RS00595 at 136.310 kb on + strand, within CA265_RS00595 at 136.310 kb on + strand, within CA265_RS00595 at 136.311 kb on - strand, within CA265_RS00595 at 136.384 kb on - strand at 136.402 kb on + strand at 136.403 kb on - strand at 136.427 kb on + strand at 136.433 kb on + strand at 136.450 kb on - strand at 136.454 kb on + strand at 136.498 kb on + strand at 136.575 kb on + strand, within CA265_RS00600 at 136.594 kb on - strand, within CA265_RS00600 at 136.632 kb on + strand, within CA265_RS00600 at 136.806 kb on + strand, within CA265_RS00600 at 136.810 kb on + strand, within CA265_RS00600 at 136.895 kb on - strand, within CA265_RS00600 at 136.911 kb on + strand, within CA265_RS00600 at 136.981 kb on + strand, within CA265_RS00600 at 137.065 kb on + strand, within CA265_RS00600 at 137.083 kb on + strand, within CA265_RS00600 at 137.119 kb on - strand, within CA265_RS00600 at 137.119 kb on - strand, within CA265_RS00600 at 137.149 kb on - strand, within CA265_RS00600 at 137.202 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.327 kb on - strand, within CA265_RS00600 at 137.334 kb on + strand, within CA265_RS00600 at 137.335 kb on - strand, within CA265_RS00600 at 137.407 kb on - strand at 137.442 kb on + strand at 137.530 kb on - strand at 137.530 kb on - strand at 137.669 kb on + strand at 137.669 kb on + strand at 137.681 kb on + strand at 137.682 kb on - strand at 137.682 kb on - strand at 137.729 kb on + strand, within CA265_RS00605 at 137.894 kb on + strand, within CA265_RS00605 at 137.900 kb on - strand, within CA265_RS00605 at 137.975 kb on - strand, within CA265_RS00605 at 137.975 kb on - strand, within CA265_RS00605 at 138.030 kb on + strand, within CA265_RS00605 at 138.133 kb on + strand, within CA265_RS00605 at 138.206 kb on + strand, within CA265_RS00605 at 138.211 kb on + strand, within CA265_RS00605 at 138.212 kb on - strand, within CA265_RS00605 at 138.296 kb on - strand at 138.316 kb on - strand at 138.413 kb on - strand at 138.427 kb on + strand at 138.474 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 135,934 - +0.6 136,148 + -0.6 136,148 + -0.4 136,158 + -0.2 136,158 + -0.4 136,158 + +0.0 136,159 - +0.3 136,308 + CA265_RS00595 0.75 +1.4 136,309 - CA265_RS00595 0.76 -0.2 136,310 + CA265_RS00595 0.76 -1.1 136,310 + CA265_RS00595 0.76 +0.7 136,310 + CA265_RS00595 0.76 +0.1 136,311 - CA265_RS00595 0.77 +0.1 136,384 - +0.4 136,402 + -0.7 136,403 - +1.1 136,427 + +0.5 136,433 + -0.0 136,450 - -1.5 136,454 + +0.2 136,498 + -0.9 136,575 + CA265_RS00600 0.10 -0.6 136,594 - CA265_RS00600 0.12 -1.0 136,632 + CA265_RS00600 0.16 -1.1 136,806 + CA265_RS00600 0.33 -0.9 136,810 + CA265_RS00600 0.33 -1.7 136,895 - CA265_RS00600 0.42 -1.1 136,911 + CA265_RS00600 0.43 +0.3 136,981 + CA265_RS00600 0.50 -3.2 137,065 + CA265_RS00600 0.59 -0.6 137,083 + CA265_RS00600 0.60 -1.6 137,119 - CA265_RS00600 0.64 -1.0 137,119 - CA265_RS00600 0.64 +0.4 137,149 - CA265_RS00600 0.67 -1.9 137,202 + CA265_RS00600 0.72 -1.9 137,244 + CA265_RS00600 0.76 +1.4 137,244 + CA265_RS00600 0.76 -1.3 137,244 + CA265_RS00600 0.76 -0.9 137,244 + CA265_RS00600 0.76 -3.0 137,327 - CA265_RS00600 0.84 -1.7 137,334 + CA265_RS00600 0.85 -0.9 137,335 - CA265_RS00600 0.85 -2.4 137,407 - -0.9 137,442 + -0.6 137,530 - -0.4 137,530 - -0.4 137,669 + -0.2 137,669 + +0.2 137,681 + +0.2 137,682 - -0.4 137,682 - -0.6 137,729 + CA265_RS00605 0.16 -0.0 137,894 + CA265_RS00605 0.41 +0.4 137,900 - CA265_RS00605 0.42 -0.1 137,975 - CA265_RS00605 0.53 -2.0 137,975 - CA265_RS00605 0.53 -1.4 138,030 + CA265_RS00605 0.62 -0.2 138,133 + CA265_RS00605 0.77 -0.2 138,206 + CA265_RS00605 0.88 -0.5 138,211 + CA265_RS00605 0.89 +1.4 138,212 - CA265_RS00605 0.89 +0.1 138,296 - +0.4 138,316 - +0.0 138,413 - +0.3 138,427 + -0.1 138,474 + -0.5
Or see this region's nucleotide sequence