Experiment: CDM1 without LipoicAcid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14295 and BBR_RS14300 are separated by 123 nucleotides BBR_RS14300 and BBR_RS14305 are separated by 172 nucleotides BBR_RS14305 and BBR_RS14310 are separated by 88 nucleotides BBR_RS14310 and BBR_RS14315 are separated by 38 nucleotides
BBR_RS14295: BBR_RS14295 - pyruvate kinase, at 982,725 to 984,167
_RS14295
BBR_RS14300: BBR_RS14300 - NUDIX hydrolase, at 984,291 to 984,890
_RS14300
BBR_RS14305: BBR_RS14305 - tRNA-Leu, at 985,063 to 985,136
_RS14305
BBR_RS14310: BBR_RS14310 - ANTAR domain-containing protein, at 985,225 to 986,013
_RS14310
BBR_RS14315: BBR_RS14315 - DNA polymerase I, at 986,052 to 988,907
_RS14315
Position (kb) 985
986 Strain fitness (log2 ratio) -2
-1
0
1 at 984.138 kb on + strand at 984.148 kb on + strand at 984.148 kb on + strand at 984.186 kb on + strand at 984.191 kb on + strand at 984.253 kb on + strand at 984.352 kb on - strand, within BBR_RS14300 at 984.421 kb on - strand, within BBR_RS14300 at 984.458 kb on + strand, within BBR_RS14300 at 984.479 kb on + strand, within BBR_RS14300 at 984.489 kb on + strand, within BBR_RS14300 at 984.489 kb on + strand, within BBR_RS14300 at 984.489 kb on + strand, within BBR_RS14300 at 984.497 kb on - strand, within BBR_RS14300 at 984.590 kb on - strand, within BBR_RS14300 at 984.919 kb on + strand at 984.919 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without LipoicAcid remove 984,138 + +1.0 984,148 + -0.9 984,148 + -1.2 984,186 + +1.2 984,191 + +0.8 984,253 + -0.2 984,352 - BBR_RS14300 0.10 +0.8 984,421 - BBR_RS14300 0.22 -1.2 984,458 + BBR_RS14300 0.28 -0.5 984,479 + BBR_RS14300 0.31 -0.4 984,489 + BBR_RS14300 0.33 -2.2 984,489 + BBR_RS14300 0.33 -1.6 984,489 + BBR_RS14300 0.33 -0.5 984,497 - BBR_RS14300 0.34 -0.0 984,590 - BBR_RS14300 0.50 -0.3 984,919 + -0.3 984,919 + -2.0
Or see this region's nucleotide sequence