Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23365 and AO353_23370 are separated by 64 nucleotides AO353_23370 and AO353_23375 are separated by 117 nucleotides AO353_23375 and AO353_23380 are separated by 14 nucleotides AO353_23380 and AO353_23385 are separated by 347 nucleotides
AO353_23365: AO353_23365 - transcriptional regulator, at 5,122,361 to 5,123,359
_23365
AO353_23370: AO353_23370 - cytochrome C, at 5,123,424 to 5,123,813
_23370
AO353_23375: AO353_23375 - Valyl-tRNA synthetase, at 5,123,931 to 5,124,260
_23375
AO353_23380: AO353_23380 - hypothetical protein, at 5,124,275 to 5,125,576
_23380
AO353_23385: AO353_23385 - trans-2-enoyl-CoA reductase, at 5,125,924 to 5,127,120
_23385
Position (kb) 5124
5125
5126 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5123.314 kb on + strand at 5123.314 kb on + strand at 5123.314 kb on + strand at 5123.314 kb on + strand at 5123.314 kb on + strand at 5123.314 kb on + strand at 5123.314 kb on + strand at 5123.314 kb on + strand at 5123.315 kb on - strand at 5123.315 kb on - strand at 5123.315 kb on - strand at 5123.315 kb on - strand at 5123.315 kb on - strand at 5123.315 kb on - strand at 5123.315 kb on - strand at 5123.320 kb on + strand at 5123.321 kb on - strand at 5123.347 kb on + strand at 5123.347 kb on + strand at 5123.348 kb on - strand at 5123.348 kb on - strand at 5123.381 kb on + strand at 5123.381 kb on + strand at 5123.381 kb on + strand at 5123.381 kb on + strand at 5123.381 kb on + strand at 5123.381 kb on + strand at 5123.381 kb on + strand at 5123.381 kb on + strand at 5123.382 kb on - strand at 5123.382 kb on - strand at 5123.382 kb on - strand at 5123.382 kb on - strand at 5123.397 kb on + strand at 5123.398 kb on - strand at 5123.401 kb on + strand at 5123.401 kb on + strand at 5123.401 kb on + strand at 5123.402 kb on - strand at 5123.402 kb on - strand at 5123.402 kb on - strand at 5123.402 kb on - strand at 5123.402 kb on - strand at 5123.402 kb on - strand at 5123.402 kb on - strand at 5123.402 kb on - strand at 5123.402 kb on - strand at 5123.402 kb on - strand at 5123.441 kb on - strand at 5123.441 kb on - strand at 5123.441 kb on - strand at 5123.443 kb on + strand at 5123.444 kb on - strand at 5123.466 kb on - strand, within AO353_23370 at 5123.466 kb on - strand, within AO353_23370 at 5123.473 kb on + strand, within AO353_23370 at 5123.474 kb on - strand, within AO353_23370 at 5123.635 kb on - strand, within AO353_23370 at 5123.640 kb on + strand, within AO353_23370 at 5123.641 kb on - strand, within AO353_23370 at 5123.697 kb on + strand, within AO353_23370 at 5123.697 kb on + strand, within AO353_23370 at 5123.697 kb on + strand, within AO353_23370 at 5123.698 kb on - strand, within AO353_23370 at 5123.698 kb on - strand, within AO353_23370 at 5123.698 kb on - strand, within AO353_23370 at 5123.698 kb on - strand, within AO353_23370 at 5123.719 kb on + strand, within AO353_23370 at 5123.720 kb on - strand, within AO353_23370 at 5123.788 kb on - strand at 5123.842 kb on - strand at 5124.023 kb on + strand, within AO353_23375 at 5124.023 kb on + strand, within AO353_23375 at 5124.024 kb on - strand, within AO353_23375 at 5124.024 kb on - strand, within AO353_23375 at 5124.090 kb on + strand, within AO353_23375 at 5124.091 kb on - strand, within AO353_23375 at 5124.117 kb on + strand, within AO353_23375 at 5124.117 kb on + strand, within AO353_23375 at 5124.117 kb on + strand, within AO353_23375 at 5124.118 kb on - strand, within AO353_23375 at 5124.174 kb on + strand, within AO353_23375 at 5124.174 kb on + strand, within AO353_23375 at 5124.174 kb on + strand, within AO353_23375 at 5124.175 kb on - strand, within AO353_23375 at 5124.222 kb on + strand, within AO353_23375 at 5124.222 kb on + strand, within AO353_23375 at 5124.222 kb on + strand, within AO353_23375 at 5124.222 kb on + strand, within AO353_23375 at 5124.223 kb on - strand at 5124.223 kb on - strand, within AO353_23375 at 5124.223 kb on - strand, within AO353_23375 at 5124.240 kb on + strand at 5124.240 kb on + strand at 5124.242 kb on + strand at 5124.242 kb on + strand at 5124.242 kb on + strand at 5124.243 kb on - strand at 5124.243 kb on - strand at 5124.243 kb on - strand at 5124.243 kb on - strand at 5124.254 kb on + strand at 5124.255 kb on - strand at 5124.272 kb on + strand at 5124.273 kb on - strand at 5124.273 kb on - strand at 5124.273 kb on - strand at 5124.276 kb on + strand at 5124.276 kb on + strand at 5124.276 kb on + strand at 5124.276 kb on + strand at 5124.277 kb on - strand at 5124.277 kb on - strand at 5124.277 kb on - strand at 5124.319 kb on + strand at 5124.319 kb on + strand at 5124.319 kb on + strand at 5124.319 kb on + strand at 5124.319 kb on + strand at 5124.320 kb on - strand at 5124.321 kb on + strand at 5124.321 kb on + strand at 5124.459 kb on + strand, within AO353_23380 at 5124.459 kb on + strand, within AO353_23380 at 5124.460 kb on - strand, within AO353_23380 at 5124.554 kb on + strand, within AO353_23380 at 5124.555 kb on - strand, within AO353_23380 at 5124.841 kb on + strand, within AO353_23380 at 5124.945 kb on + strand, within AO353_23380 at 5124.945 kb on + strand, within AO353_23380 at 5124.945 kb on + strand, within AO353_23380 at 5124.945 kb on + strand, within AO353_23380 at 5124.945 kb on + strand, within AO353_23380 at 5124.945 kb on + strand, within AO353_23380 at 5124.945 kb on + strand, within AO353_23380 at 5124.945 kb on + strand at 5124.945 kb on + strand, within AO353_23380 at 5124.946 kb on - strand, within AO353_23380 at 5124.946 kb on - strand, within AO353_23380 at 5124.946 kb on - strand, within AO353_23380 at 5124.946 kb on - strand, within AO353_23380 at 5124.946 kb on - strand, within AO353_23380 at 5124.946 kb on - strand, within AO353_23380 at 5125.057 kb on - strand, within AO353_23380 at 5125.072 kb on + strand, within AO353_23380 at 5125.072 kb on + strand, within AO353_23380 at 5125.073 kb on - strand, within AO353_23380 at 5125.073 kb on - strand, within AO353_23380 at 5125.101 kb on + strand at 5125.101 kb on + strand, within AO353_23380 at 5125.101 kb on + strand, within AO353_23380 at 5125.101 kb on + strand, within AO353_23380 at 5125.101 kb on + strand, within AO353_23380 at 5125.101 kb on + strand, within AO353_23380 at 5125.101 kb on + strand, within AO353_23380 at 5125.101 kb on + strand, within AO353_23380 at 5125.101 kb on + strand, within AO353_23380 at 5125.101 kb on + strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.102 kb on - strand, within AO353_23380 at 5125.131 kb on + strand, within AO353_23380 at 5125.131 kb on + strand, within AO353_23380 at 5125.194 kb on + strand, within AO353_23380 at 5125.194 kb on + strand, within AO353_23380 at 5125.194 kb on + strand at 5125.194 kb on + strand, within AO353_23380 at 5125.195 kb on - strand, within AO353_23380 at 5125.195 kb on - strand, within AO353_23380 at 5125.195 kb on - strand, within AO353_23380 at 5125.195 kb on - strand, within AO353_23380 at 5125.195 kb on - strand, within AO353_23380 at 5125.198 kb on + strand, within AO353_23380 at 5125.198 kb on + strand, within AO353_23380 at 5125.198 kb on + strand, within AO353_23380 at 5125.198 kb on + strand, within AO353_23380 at 5125.198 kb on + strand, within AO353_23380 at 5125.199 kb on - strand, within AO353_23380 at 5125.199 kb on - strand, within AO353_23380 at 5125.199 kb on - strand, within AO353_23380 at 5125.199 kb on - strand, within AO353_23380 at 5125.199 kb on - strand, within AO353_23380 at 5125.199 kb on - strand, within AO353_23380 at 5125.199 kb on - strand, within AO353_23380 at 5125.335 kb on + strand, within AO353_23380 at 5125.336 kb on - strand, within AO353_23380 at 5125.336 kb on - strand, within AO353_23380 at 5125.411 kb on + strand, within AO353_23380 at 5125.411 kb on + strand, within AO353_23380 at 5125.411 kb on + strand, within AO353_23380 at 5125.411 kb on + strand, within AO353_23380 at 5125.411 kb on + strand, within AO353_23380 at 5125.412 kb on - strand, within AO353_23380 at 5125.412 kb on - strand, within AO353_23380 at 5125.412 kb on - strand, within AO353_23380 at 5125.412 kb on - strand, within AO353_23380 at 5125.596 kb on + strand at 5125.596 kb on + strand at 5125.596 kb on + strand at 5125.596 kb on + strand at 5125.596 kb on + strand at 5125.596 kb on + strand at 5125.596 kb on + strand at 5125.596 kb on + strand at 5125.596 kb on + strand at 5125.596 kb on + strand at 5125.597 kb on - strand at 5125.597 kb on - strand at 5125.597 kb on - strand at 5125.597 kb on - strand at 5125.597 kb on - strand at 5125.597 kb on - strand at 5125.597 kb on - strand at 5125.597 kb on - strand at 5125.674 kb on - strand at 5125.674 kb on - strand at 5125.685 kb on + strand at 5125.728 kb on + strand at 5125.729 kb on - strand at 5125.729 kb on - strand at 5125.788 kb on + strand at 5125.788 kb on + strand at 5125.788 kb on + strand at 5125.870 kb on + strand at 5125.879 kb on + strand at 5125.879 kb on + strand at 5125.879 kb on + strand at 5125.880 kb on - strand at 5125.880 kb on - strand at 5125.880 kb on - strand at 5125.889 kb on - strand at 5125.919 kb on + strand at 5125.919 kb on + strand at 5125.919 kb on + strand at 5125.919 kb on + strand at 5125.920 kb on - strand at 5125.920 kb on - strand at 5125.920 kb on - strand at 5125.920 kb on - strand at 5125.920 kb on - strand at 5125.923 kb on + strand at 5125.923 kb on + strand at 5125.923 kb on + strand at 5125.923 kb on + strand at 5125.923 kb on + strand at 5125.924 kb on - strand at 5125.924 kb on - strand at 5125.924 kb on - strand at 5125.924 kb on - strand at 5126.011 kb on + strand at 5126.189 kb on - strand, within AO353_23385 at 5126.189 kb on - strand, within AO353_23385 at 5126.189 kb on - strand, within AO353_23385 at 5126.234 kb on - strand, within AO353_23385 at 5126.380 kb on + strand, within AO353_23385 at 5126.380 kb on + strand, within AO353_23385 at 5126.497 kb on - strand, within AO353_23385
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 5,123,314 + +0.9 5,123,314 + -1.2 5,123,314 + -0.1 5,123,314 + -0.4 5,123,314 + -1.1 5,123,314 + -0.2 5,123,314 + +0.6 5,123,314 + -1.2 5,123,315 - +0.5 5,123,315 - -0.5 5,123,315 - -1.1 5,123,315 - -1.4 5,123,315 - +2.5 5,123,315 - +0.7 5,123,315 - -1.2 5,123,320 + -0.6 5,123,321 - -0.1 5,123,347 + +0.2 5,123,347 + -1.0 5,123,348 - -0.4 5,123,348 - -0.2 5,123,381 + -1.2 5,123,381 + +0.1 5,123,381 + -0.5 5,123,381 + +0.9 5,123,381 + -0.2 5,123,381 + +1.3 5,123,381 + -0.9 5,123,381 + +0.5 5,123,382 - +0.4 5,123,382 - -0.5 5,123,382 - -1.2 5,123,382 - -1.6 5,123,397 + +0.2 5,123,398 - -2.1 5,123,401 + +0.2 5,123,401 + -0.6 5,123,401 + -1.4 5,123,402 - +0.9 5,123,402 - -0.5 5,123,402 - -0.4 5,123,402 - -1.1 5,123,402 - +0.1 5,123,402 - +0.3 5,123,402 - +0.5 5,123,402 - -1.9 5,123,402 - +0.2 5,123,402 - -1.1 5,123,441 - +0.4 5,123,441 - -1.3 5,123,441 - +0.7 5,123,443 + -0.8 5,123,444 - +0.7 5,123,466 - AO353_23370 0.11 -0.3 5,123,466 - AO353_23370 0.11 -1.3 5,123,473 + AO353_23370 0.13 -2.2 5,123,474 - AO353_23370 0.13 -0.2 5,123,635 - AO353_23370 0.54 +0.1 5,123,640 + AO353_23370 0.55 +0.9 5,123,641 - AO353_23370 0.56 -0.3 5,123,697 + AO353_23370 0.70 -1.3 5,123,697 + AO353_23370 0.70 +1.2 5,123,697 + AO353_23370 0.70 +0.5 5,123,698 - AO353_23370 0.70 +0.7 5,123,698 - AO353_23370 0.70 -0.5 5,123,698 - AO353_23370 0.70 -0.4 5,123,698 - AO353_23370 0.70 -1.6 5,123,719 + AO353_23370 0.76 -0.7 5,123,720 - AO353_23370 0.76 -0.2 5,123,788 - +0.2 5,123,842 - -0.1 5,124,023 + AO353_23375 0.28 -1.5 5,124,023 + AO353_23375 0.28 +2.5 5,124,024 - AO353_23375 0.28 +0.6 5,124,024 - AO353_23375 0.28 -1.1 5,124,090 + AO353_23375 0.48 -0.5 5,124,091 - AO353_23375 0.48 +1.9 5,124,117 + AO353_23375 0.56 +0.6 5,124,117 + AO353_23375 0.56 +0.7 5,124,117 + AO353_23375 0.56 +0.4 5,124,118 - AO353_23375 0.57 +0.5 5,124,174 + AO353_23375 0.74 -1.3 5,124,174 + AO353_23375 0.74 -0.8 5,124,174 + AO353_23375 0.74 -1.1 5,124,175 - AO353_23375 0.74 -0.3 5,124,222 + AO353_23375 0.88 -1.2 5,124,222 + AO353_23375 0.88 -1.6 5,124,222 + AO353_23375 0.88 +2.3 5,124,222 + AO353_23375 0.88 -0.2 5,124,223 - +1.4 5,124,223 - AO353_23375 0.88 +0.4 5,124,223 - AO353_23375 0.88 -0.1 5,124,240 + +0.4 5,124,240 + +0.6 5,124,242 + +0.4 5,124,242 + -0.8 5,124,242 + -0.1 5,124,243 - +0.5 5,124,243 - +0.4 5,124,243 - -0.1 5,124,243 - +0.9 5,124,254 + -1.1 5,124,255 - -1.4 5,124,272 + -1.4 5,124,273 - -0.7 5,124,273 - +0.9 5,124,273 - -0.3 5,124,276 + +0.1 5,124,276 + -0.1 5,124,276 + -0.5 5,124,276 + +0.5 5,124,277 - -1.5 5,124,277 - -2.8 5,124,277 - -1.1 5,124,319 + -0.5 5,124,319 + -0.2 5,124,319 + +0.1 5,124,319 + -0.3 5,124,319 + +0.0 5,124,320 - +0.7 5,124,321 + -0.3 5,124,321 + -0.2 5,124,459 + AO353_23380 0.14 -0.3 5,124,459 + AO353_23380 0.14 +0.9 5,124,460 - AO353_23380 0.14 -0.1 5,124,554 + AO353_23380 0.21 +0.5 5,124,555 - AO353_23380 0.22 -0.5 5,124,841 + AO353_23380 0.43 -1.3 5,124,945 + AO353_23380 0.51 -0.9 5,124,945 + AO353_23380 0.51 -0.4 5,124,945 + AO353_23380 0.51 -0.2 5,124,945 + AO353_23380 0.51 +0.8 5,124,945 + AO353_23380 0.51 -0.4 5,124,945 + AO353_23380 0.51 -0.1 5,124,945 + AO353_23380 0.51 +0.6 5,124,945 + +0.9 5,124,945 + AO353_23380 0.51 +1.9 5,124,946 - AO353_23380 0.52 -0.1 5,124,946 - AO353_23380 0.52 -0.9 5,124,946 - AO353_23380 0.52 -2.2 5,124,946 - AO353_23380 0.52 -0.1 5,124,946 - AO353_23380 0.52 -0.3 5,124,946 - AO353_23380 0.52 -0.9 5,125,057 - AO353_23380 0.60 -1.9 5,125,072 + AO353_23380 0.61 +0.6 5,125,072 + AO353_23380 0.61 -0.3 5,125,073 - AO353_23380 0.61 -0.1 5,125,073 - AO353_23380 0.61 +0.9 5,125,101 + +0.9 5,125,101 + AO353_23380 0.63 -0.1 5,125,101 + AO353_23380 0.63 +0.6 5,125,101 + AO353_23380 0.63 +0.9 5,125,101 + AO353_23380 0.63 +0.2 5,125,101 + AO353_23380 0.63 -0.1 5,125,101 + AO353_23380 0.63 +0.9 5,125,101 + AO353_23380 0.63 -0.5 5,125,101 + AO353_23380 0.63 +0.1 5,125,101 + AO353_23380 0.63 -2.1 5,125,102 - AO353_23380 0.64 +0.7 5,125,102 - AO353_23380 0.64 +0.1 5,125,102 - AO353_23380 0.64 +1.3 5,125,102 - AO353_23380 0.64 -0.6 5,125,102 - AO353_23380 0.64 +1.9 5,125,102 - AO353_23380 0.64 -0.4 5,125,102 - AO353_23380 0.64 +0.4 5,125,102 - AO353_23380 0.64 +0.2 5,125,102 - AO353_23380 0.64 +0.6 5,125,102 - AO353_23380 0.64 +0.4 5,125,102 - AO353_23380 0.64 +0.2 5,125,102 - AO353_23380 0.64 -0.5 5,125,102 - AO353_23380 0.64 -1.2 5,125,131 + AO353_23380 0.66 +1.1 5,125,131 + AO353_23380 0.66 -0.1 5,125,194 + AO353_23380 0.71 +0.3 5,125,194 + AO353_23380 0.71 +0.6 5,125,194 + -0.1 5,125,194 + AO353_23380 0.71 -0.5 5,125,195 - AO353_23380 0.71 +0.4 5,125,195 - AO353_23380 0.71 +0.7 5,125,195 - AO353_23380 0.71 -0.2 5,125,195 - AO353_23380 0.71 +0.9 5,125,195 - AO353_23380 0.71 +0.1 5,125,198 + AO353_23380 0.71 -0.6 5,125,198 + AO353_23380 0.71 +0.1 5,125,198 + AO353_23380 0.71 -1.1 5,125,198 + AO353_23380 0.71 -0.0 5,125,198 + AO353_23380 0.71 +1.2 5,125,199 - AO353_23380 0.71 -0.1 5,125,199 - AO353_23380 0.71 +1.3 5,125,199 - AO353_23380 0.71 +1.2 5,125,199 - AO353_23380 0.71 +0.9 5,125,199 - AO353_23380 0.71 +0.1 5,125,199 - AO353_23380 0.71 +0.6 5,125,199 - AO353_23380 0.71 +1.5 5,125,335 + AO353_23380 0.81 -0.1 5,125,336 - AO353_23380 0.81 +0.7 5,125,336 - AO353_23380 0.81 +1.1 5,125,411 + AO353_23380 0.87 -0.6 5,125,411 + AO353_23380 0.87 +0.9 5,125,411 + AO353_23380 0.87 -0.2 5,125,411 + AO353_23380 0.87 -0.3 5,125,411 + AO353_23380 0.87 +0.4 5,125,412 - AO353_23380 0.87 +1.2 5,125,412 - AO353_23380 0.87 +0.1 5,125,412 - AO353_23380 0.87 -0.6 5,125,412 - AO353_23380 0.87 +0.2 5,125,596 + -1.5 5,125,596 + +0.4 5,125,596 + -0.2 5,125,596 + +1.5 5,125,596 + +1.8 5,125,596 + -0.1 5,125,596 + -2.4 5,125,596 + -0.4 5,125,596 + -0.5 5,125,596 + -0.1 5,125,597 - +0.5 5,125,597 - -1.1 5,125,597 - -0.3 5,125,597 - -0.6 5,125,597 - -0.1 5,125,597 - -0.4 5,125,597 - +0.2 5,125,597 - -0.1 5,125,674 - -1.1 5,125,674 - +0.5 5,125,685 + -0.2 5,125,728 + -0.1 5,125,729 - +0.5 5,125,729 - -0.1 5,125,788 + -2.1 5,125,788 + -0.9 5,125,788 + +0.9 5,125,870 + -1.1 5,125,879 + +1.2 5,125,879 + -0.1 5,125,879 + +1.2 5,125,880 - -0.6 5,125,880 - +0.2 5,125,880 - -0.6 5,125,889 - -0.8 5,125,919 + +0.4 5,125,919 + -0.5 5,125,919 + -0.2 5,125,919 + -0.7 5,125,920 - +0.0 5,125,920 - +0.1 5,125,920 - -0.4 5,125,920 - -0.4 5,125,920 - +0.6 5,125,923 + -0.5 5,125,923 + -0.6 5,125,923 + +0.8 5,125,923 + +0.2 5,125,923 + +0.2 5,125,924 - -1.3 5,125,924 - +0.5 5,125,924 - -1.2 5,125,924 - +1.2 5,126,011 + -0.6 5,126,189 - AO353_23385 0.22 +1.4 5,126,189 - AO353_23385 0.22 +1.1 5,126,189 - AO353_23385 0.22 +0.2 5,126,234 - AO353_23385 0.26 -0.4 5,126,380 + AO353_23385 0.38 -2.4 5,126,380 + AO353_23385 0.38 -0.2 5,126,497 - AO353_23385 0.48 +1.8
Or see this region's nucleotide sequence