Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_12645 and AO353_12650 overlap by 17 nucleotides AO353_12650 and AO353_12655 are separated by 200 nucleotides AO353_12655 and AO353_12660 overlap by 8 nucleotides
AO353_12645: AO353_12645 - hypothetical protein, at 2,783,298 to 2,785,637
_12645
AO353_12650: AO353_12650 - hypothetical protein, at 2,785,621 to 2,786,187
_12650
AO353_12655: AO353_12655 - thioesterase, at 2,786,388 to 2,786,813
_12655
AO353_12660: AO353_12660 - histidine ammonia-lyase, at 2,786,806 to 2,788,350
_12660
Position (kb)
2785
2786
2787 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2784.667 kb on + strand, within AO353_12645 at 2784.667 kb on + strand, within AO353_12645 at 2784.667 kb on + strand, within AO353_12645 at 2784.668 kb on - strand, within AO353_12645 at 2784.668 kb on - strand, within AO353_12645 at 2784.668 kb on - strand, within AO353_12645 at 2784.668 kb on - strand, within AO353_12645 at 2784.668 kb on - strand, within AO353_12645 at 2784.668 kb on - strand, within AO353_12645 at 2784.734 kb on + strand, within AO353_12645 at 2784.735 kb on - strand, within AO353_12645 at 2784.735 kb on - strand, within AO353_12645 at 2784.878 kb on + strand, within AO353_12645 at 2784.879 kb on - strand, within AO353_12645 at 2784.916 kb on + strand, within AO353_12645 at 2784.916 kb on + strand, within AO353_12645 at 2784.939 kb on + strand, within AO353_12645 at 2784.939 kb on + strand, within AO353_12645 at 2784.940 kb on - strand, within AO353_12645 at 2784.940 kb on - strand, within AO353_12645 at 2784.940 kb on - strand, within AO353_12645 at 2785.215 kb on + strand, within AO353_12645 at 2785.215 kb on + strand, within AO353_12645 at 2785.215 kb on + strand, within AO353_12645 at 2785.216 kb on - strand, within AO353_12645 at 2785.216 kb on - strand, within AO353_12645 at 2785.216 kb on - strand, within AO353_12645 at 2785.216 kb on - strand, within AO353_12645 at 2785.398 kb on - strand, within AO353_12645 at 2785.398 kb on - strand, within AO353_12645 at 2785.418 kb on + strand at 2785.419 kb on - strand at 2785.419 kb on - strand at 2785.419 kb on - strand at 2786.039 kb on - strand, within AO353_12650 at 2786.197 kb on + strand at 2786.197 kb on + strand at 2786.197 kb on + strand at 2786.197 kb on + strand at 2786.314 kb on - strand at 2786.382 kb on + strand at 2786.382 kb on + strand at 2786.382 kb on + strand at 2786.383 kb on - strand at 2786.383 kb on - strand at 2786.417 kb on + strand at 2786.527 kb on + strand, within AO353_12655 at 2786.528 kb on - strand, within AO353_12655 at 2786.608 kb on + strand, within AO353_12655 at 2786.608 kb on + strand, within AO353_12655 at 2786.608 kb on + strand, within AO353_12655 at 2786.608 kb on + strand, within AO353_12655 at 2786.609 kb on - strand, within AO353_12655 at 2786.609 kb on - strand, within AO353_12655 at 2786.609 kb on - strand, within AO353_12655 at 2786.665 kb on + strand, within AO353_12655 at 2786.665 kb on + strand, within AO353_12655 at 2786.665 kb on + strand, within AO353_12655 at 2786.665 kb on + strand, within AO353_12655 at 2786.665 kb on + strand, within AO353_12655 at 2786.666 kb on - strand, within AO353_12655 at 2786.666 kb on - strand, within AO353_12655 at 2786.666 kb on - strand, within AO353_12655 at 2786.666 kb on - strand, within AO353_12655 at 2786.666 kb on - strand, within AO353_12655 at 2786.666 kb on - strand, within AO353_12655 at 2786.666 kb on - strand at 2786.713 kb on + strand, within AO353_12655 at 2786.713 kb on + strand, within AO353_12655 at 2786.713 kb on + strand, within AO353_12655 at 2786.722 kb on + strand, within AO353_12655 at 2786.722 kb on + strand, within AO353_12655 at 2786.722 kb on + strand, within AO353_12655 at 2786.722 kb on + strand, within AO353_12655 at 2786.722 kb on + strand, within AO353_12655 at 2786.722 kb on + strand, within AO353_12655 at 2786.722 kb on + strand, within AO353_12655 at 2786.723 kb on - strand, within AO353_12655 at 2786.723 kb on - strand, within AO353_12655 at 2786.723 kb on - strand, within AO353_12655 at 2786.807 kb on + strand at 2786.808 kb on - strand at 2786.808 kb on - strand at 2787.181 kb on - strand, within AO353_12660 at 2787.181 kb on - strand, within AO353_12660 at 2787.181 kb on - strand, within AO353_12660
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 2,784,667 + AO353_12645 0.59 +0.6 2,784,667 + AO353_12645 0.59 -0.1 2,784,667 + AO353_12645 0.59 -1.5 2,784,668 - AO353_12645 0.59 +1.9 2,784,668 - AO353_12645 0.59 -0.4 2,784,668 - AO353_12645 0.59 -0.3 2,784,668 - AO353_12645 0.59 -0.4 2,784,668 - AO353_12645 0.59 -0.3 2,784,668 - AO353_12645 0.59 +0.9 2,784,734 + AO353_12645 0.61 +1.9 2,784,735 - AO353_12645 0.61 +0.2 2,784,735 - AO353_12645 0.61 -1.3 2,784,878 + AO353_12645 0.68 +0.9 2,784,879 - AO353_12645 0.68 +0.5 2,784,916 + AO353_12645 0.69 -0.5 2,784,916 + AO353_12645 0.69 +2.5 2,784,939 + AO353_12645 0.70 +0.4 2,784,939 + AO353_12645 0.70 +0.9 2,784,940 - AO353_12645 0.70 +1.5 2,784,940 - AO353_12645 0.70 -0.4 2,784,940 - AO353_12645 0.70 +0.5 2,785,215 + AO353_12645 0.82 +0.5 2,785,215 + AO353_12645 0.82 -1.3 2,785,215 + AO353_12645 0.82 +0.6 2,785,216 - AO353_12645 0.82 +0.7 2,785,216 - AO353_12645 0.82 -0.6 2,785,216 - AO353_12645 0.82 -1.1 2,785,216 - AO353_12645 0.82 -0.1 2,785,398 - AO353_12645 0.90 -0.8 2,785,398 - AO353_12645 0.90 +0.4 2,785,418 + -2.2 2,785,419 - +0.8 2,785,419 - +0.4 2,785,419 - -0.9 2,786,039 - AO353_12650 0.74 -1.6 2,786,197 + +0.1 2,786,197 + -0.3 2,786,197 + -1.1 2,786,197 + -0.2 2,786,314 - -0.3 2,786,382 + -0.1 2,786,382 + -0.8 2,786,382 + -1.1 2,786,383 - +0.9 2,786,383 - -0.1 2,786,417 + +1.7 2,786,527 + AO353_12655 0.33 +0.2 2,786,528 - AO353_12655 0.33 +2.3 2,786,608 + AO353_12655 0.52 -0.0 2,786,608 + AO353_12655 0.52 +0.4 2,786,608 + AO353_12655 0.52 -0.1 2,786,608 + AO353_12655 0.52 +0.8 2,786,609 - AO353_12655 0.52 +1.4 2,786,609 - AO353_12655 0.52 -0.4 2,786,609 - AO353_12655 0.52 +0.5 2,786,665 + AO353_12655 0.65 +0.3 2,786,665 + AO353_12655 0.65 -0.6 2,786,665 + AO353_12655 0.65 -0.3 2,786,665 + AO353_12655 0.65 -1.1 2,786,665 + AO353_12655 0.65 -0.6 2,786,666 - AO353_12655 0.65 +0.2 2,786,666 - AO353_12655 0.65 -0.5 2,786,666 - AO353_12655 0.65 +1.9 2,786,666 - AO353_12655 0.65 -0.1 2,786,666 - AO353_12655 0.65 +1.1 2,786,666 - AO353_12655 0.65 +1.3 2,786,666 - +2.9 2,786,713 + AO353_12655 0.76 -0.7 2,786,713 + AO353_12655 0.76 -0.9 2,786,713 + AO353_12655 0.76 +0.6 2,786,722 + AO353_12655 0.78 -0.1 2,786,722 + AO353_12655 0.78 +0.9 2,786,722 + AO353_12655 0.78 +0.5 2,786,722 + AO353_12655 0.78 +1.0 2,786,722 + AO353_12655 0.78 -0.3 2,786,722 + AO353_12655 0.78 -0.1 2,786,722 + AO353_12655 0.78 +0.4 2,786,723 - AO353_12655 0.79 +0.0 2,786,723 - AO353_12655 0.79 -0.2 2,786,723 - AO353_12655 0.79 -1.5 2,786,807 + -0.9 2,786,808 - -0.8 2,786,808 - -1.1 2,787,181 - AO353_12660 0.24 +1.5 2,787,181 - AO353_12660 0.24 +0.7 2,787,181 - AO353_12660 0.24 -0.5
Or see this region's nucleotide sequence