Experiment: Cobalt chloride 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0020 and Shewana3_0021 are separated by 35 nucleotides Shewana3_0021 and Shewana3_0022 are separated by 152 nucleotides Shewana3_0022 and fadA are separated by 224 nucleotides
Shewana3_0020: Shewana3_0020 - MOSC domain-containing protein (RefSeq), at 25,944 to 26,381
_0020
Shewana3_0021: Shewana3_0021 - sulfur transfer protein SirA (RefSeq), at 26,417 to 26,662
_0021
Shewana3_0022: Shewana3_0022 - diguanylate cyclase (RefSeq), at 26,815 to 28,026
_0022
Shewana3_0023: fadA - Acetyl-CoA C-acyltransferase (EC 2.3.1.16) <small>(from data)</small>, at 28,251 to 29,414
fadA
Position (kb) 26
27
28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 25.854 kb on - strand at 25.896 kb on + strand at 25.912 kb on + strand at 25.919 kb on - strand at 25.929 kb on - strand at 25.955 kb on - strand at 25.955 kb on - strand at 26.087 kb on - strand, within Shewana3_0020 at 26.111 kb on - strand, within Shewana3_0020 at 26.250 kb on + strand, within Shewana3_0020 at 26.293 kb on + strand, within Shewana3_0020 at 26.301 kb on - strand, within Shewana3_0020 at 26.301 kb on - strand, within Shewana3_0020 at 26.301 kb on - strand, within Shewana3_0020 at 26.398 kb on - strand at 26.757 kb on + strand at 26.765 kb on - strand at 26.804 kb on + strand at 26.882 kb on + strand at 26.890 kb on - strand at 26.957 kb on - strand, within Shewana3_0022 at 27.167 kb on + strand, within Shewana3_0022 at 27.167 kb on + strand, within Shewana3_0022 at 27.175 kb on - strand, within Shewana3_0022 at 27.242 kb on + strand, within Shewana3_0022 at 27.279 kb on - strand, within Shewana3_0022 at 27.314 kb on - strand, within Shewana3_0022 at 27.315 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.348 kb on - strand, within Shewana3_0022 at 27.381 kb on + strand, within Shewana3_0022 at 27.438 kb on + strand, within Shewana3_0022 at 27.446 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.831 kb on + strand, within Shewana3_0022 at 27.850 kb on + strand, within Shewana3_0022 at 27.978 kb on + strand at 28.041 kb on - strand at 28.069 kb on + strand at 28.069 kb on + strand at 28.069 kb on + strand at 28.071 kb on + strand at 28.077 kb on - strand at 28.077 kb on - strand at 28.077 kb on - strand at 28.143 kb on - strand at 28.169 kb on + strand at 28.249 kb on + strand at 28.256 kb on - strand at 28.283 kb on + strand at 28.283 kb on + strand at 28.292 kb on - strand at 28.375 kb on - strand, within fadA at 28.377 kb on + strand, within fadA at 28.385 kb on - strand, within fadA at 28.418 kb on - strand, within fadA at 28.428 kb on - strand, within fadA at 28.438 kb on - strand, within fadA at 28.445 kb on + strand, within fadA at 28.445 kb on + strand, within fadA at 28.454 kb on + strand, within fadA at 28.464 kb on + strand, within fadA at 28.473 kb on - strand, within fadA at 28.473 kb on - strand, within fadA at 28.473 kb on - strand, within fadA at 28.734 kb on + strand, within fadA at 28.742 kb on - strand, within fadA at 28.804 kb on + strand, within fadA at 28.841 kb on - strand, within fadA at 28.874 kb on - strand, within fadA at 28.903 kb on - strand, within fadA at 28.931 kb on + strand, within fadA at 28.947 kb on - strand, within fadA at 29.012 kb on - strand, within fadA
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.16 mM remove 25,854 - -0.4 25,896 + -0.2 25,912 + -0.2 25,919 - -0.6 25,929 - -0.1 25,955 - -0.2 25,955 - -0.2 26,087 - Shewana3_0020 0.33 -0.2 26,111 - Shewana3_0020 0.38 -0.1 26,250 + Shewana3_0020 0.70 -0.5 26,293 + Shewana3_0020 0.80 -3.2 26,301 - Shewana3_0020 0.82 -0.0 26,301 - Shewana3_0020 0.82 +0.4 26,301 - Shewana3_0020 0.82 -0.1 26,398 - +0.0 26,757 + -0.4 26,765 - -0.4 26,804 + -0.2 26,882 + -0.0 26,890 - -1.4 26,957 - Shewana3_0022 0.12 -1.1 27,167 + Shewana3_0022 0.29 -0.7 27,167 + Shewana3_0022 0.29 -0.3 27,175 - Shewana3_0022 0.30 +1.2 27,242 + Shewana3_0022 0.35 -1.7 27,279 - Shewana3_0022 0.38 -0.0 27,314 - Shewana3_0022 0.41 -0.9 27,315 + Shewana3_0022 0.41 -0.7 27,340 + Shewana3_0022 0.43 -0.3 27,340 + Shewana3_0022 0.43 -0.5 27,340 + Shewana3_0022 0.43 +0.2 27,348 - Shewana3_0022 0.44 -1.5 27,381 + Shewana3_0022 0.47 -0.1 27,438 + Shewana3_0022 0.51 -0.0 27,446 - Shewana3_0022 0.52 -1.1 27,763 - Shewana3_0022 0.78 -1.1 27,763 - Shewana3_0022 0.78 +0.4 27,763 - Shewana3_0022 0.78 -0.0 27,763 - Shewana3_0022 0.78 -0.4 27,831 + Shewana3_0022 0.84 +0.5 27,850 + Shewana3_0022 0.85 +0.4 27,978 + +0.4 28,041 - -0.1 28,069 + -0.4 28,069 + +0.1 28,069 + +0.4 28,071 + -0.1 28,077 - -0.0 28,077 - +0.2 28,077 - -0.0 28,143 - -0.1 28,169 + -0.2 28,249 + -0.9 28,256 - +0.1 28,283 + -0.1 28,283 + +0.3 28,292 - -0.1 28,375 - fadA Shewana3_0023 0.11 +1.3 28,377 + fadA Shewana3_0023 0.11 -0.1 28,385 - fadA Shewana3_0023 0.12 -0.1 28,418 - fadA Shewana3_0023 0.14 +0.2 28,428 - fadA Shewana3_0023 0.15 +0.1 28,438 - fadA Shewana3_0023 0.16 +0.1 28,445 + fadA Shewana3_0023 0.17 -0.5 28,445 + fadA Shewana3_0023 0.17 -0.3 28,454 + fadA Shewana3_0023 0.17 -0.4 28,464 + fadA Shewana3_0023 0.18 +0.2 28,473 - fadA Shewana3_0023 0.19 +0.3 28,473 - fadA Shewana3_0023 0.19 +0.1 28,473 - fadA Shewana3_0023 0.19 -0.2 28,734 + fadA Shewana3_0023 0.41 +0.0 28,742 - fadA Shewana3_0023 0.42 -0.6 28,804 + fadA Shewana3_0023 0.48 +0.0 28,841 - fadA Shewana3_0023 0.51 +0.2 28,874 - fadA Shewana3_0023 0.54 +0.2 28,903 - fadA Shewana3_0023 0.56 +0.2 28,931 + fadA Shewana3_0023 0.58 -0.2 28,947 - fadA Shewana3_0023 0.60 -0.1 29,012 - fadA Shewana3_0023 0.65 +0.1
Or see this region's nucleotide sequence