Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2647 and Shew_2648 overlap by 8 nucleotides Shew_2648 and Shew_2649 are separated by 140 nucleotides Shew_2649 and Shew_2650 are separated by 149 nucleotides
Shew_2647: Shew_2647 - hypothetical protein (RefSeq), at 3,123,264 to 3,123,563
_2647
Shew_2648: Shew_2648 - GCN5-related N-acetyltransferase (RefSeq), at 3,123,556 to 3,124,206
_2648
Shew_2649: Shew_2649 - hypothetical protein (RefSeq), at 3,124,347 to 3,124,577
_2649
Shew_2650: Shew_2650 - hypothetical protein (RefSeq), at 3,124,727 to 3,125,098
_2650
Position (kb) 3123
3124
3125 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3123.641 kb on + strand, within Shew_2648 at 3123.991 kb on + strand, within Shew_2648 at 3124.190 kb on - strand at 3124.222 kb on + strand at 3124.307 kb on + strand at 3124.875 kb on + strand, within Shew_2650 at 3124.883 kb on - strand, within Shew_2650 at 3125.009 kb on - strand, within Shew_2650 at 3125.055 kb on - strand, within Shew_2650 at 3125.055 kb on - strand, within Shew_2650
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,123,641 + Shew_2648 0.13 -2.8 3,123,991 + Shew_2648 0.67 -0.5 3,124,190 - -0.3 3,124,222 + +0.1 3,124,307 + -0.3 3,124,875 + Shew_2650 0.40 +0.2 3,124,883 - Shew_2650 0.42 +0.1 3,125,009 - Shew_2650 0.76 -0.8 3,125,055 - Shew_2650 0.88 +1.0 3,125,055 - Shew_2650 0.88 -0.1
Or see this region's nucleotide sequence