Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0564 and Shew_0565 overlap by 8 nucleotides Shew_0565 and Shew_0566 are separated by 92 nucleotides Shew_0566 and Shew_0567 are separated by 20 nucleotides
Shew_0564: Shew_0564 - DNA mismatch repair protein MutL (RefSeq), at 639,265 to 641,160
_0564
Shew_0565: Shew_0565 - tRNA delta(2)-isopentenylpyrophosphate transferase (RefSeq), at 641,153 to 642,079
_0565
Shew_0566: Shew_0566 - RNA chaperone Hfq (RefSeq), at 642,172 to 642,450
_0566
Shew_0567: Shew_0567 - GTP-binding protein, HSR1-related (RefSeq), at 642,471 to 643,769
_0567
Position (kb) 641
642
643 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 640.178 kb on + strand, within Shew_0564 at 640.186 kb on - strand, within Shew_0564 at 640.225 kb on - strand, within Shew_0564 at 640.519 kb on + strand, within Shew_0564 at 640.586 kb on - strand, within Shew_0564 at 640.643 kb on + strand, within Shew_0564 at 640.699 kb on + strand, within Shew_0564 at 641.242 kb on + strand at 641.590 kb on + strand, within Shew_0565 at 641.717 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.987 kb on + strand at 642.371 kb on + strand, within Shew_0566 at 642.499 kb on + strand at 642.504 kb on + strand at 642.538 kb on + strand at 642.839 kb on + strand, within Shew_0567
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 640,178 + Shew_0564 0.48 +0.3 640,186 - Shew_0564 0.49 -1.5 640,225 - Shew_0564 0.51 -0.7 640,519 + Shew_0564 0.66 +0.3 640,586 - Shew_0564 0.70 +1.1 640,643 + Shew_0564 0.73 +0.1 640,699 + Shew_0564 0.76 +0.5 641,242 + -2.1 641,590 + Shew_0565 0.47 -1.2 641,717 + Shew_0565 0.61 -2.0 641,768 + Shew_0565 0.66 -1.8 641,768 + Shew_0565 0.66 -0.9 641,987 + -1.2 642,371 + Shew_0566 0.71 -3.1 642,499 + -0.8 642,504 + -0.8 642,538 + -1.7 642,839 + Shew_0567 0.28 -0.7
Or see this region's nucleotide sequence