Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0560 and Shew_0561 are separated by 436 nucleotides Shew_0561 and Shew_0562 are separated by 100 nucleotides Shew_0562 and Shew_0563 overlap by 14 nucleotides
Shew_0560: Shew_0560 - hypothetical protein (RefSeq), at 635,005 to 635,460
_0560
Shew_0561: Shew_0561 - carbohydrate kinase, YjeF-related protein (RefSeq), at 635,897 to 637,375
_0561
Shew_0562: Shew_0562 - hypothetical protein (RefSeq), at 637,476 to 637,949
_0562
Shew_0563: Shew_0563 - N-acetylmuramoyl-L-alanine amidase (RefSeq), at 637,936 to 639,255
_0563
Position (kb)
635
636
637
638 Strain fitness (log2 ratio)
-2
-1
0
1 at 635.031 kb on + strand at 635.049 kb on - strand at 635.088 kb on - strand, within Shew_0560 at 635.090 kb on + strand, within Shew_0560 at 635.090 kb on + strand, within Shew_0560 at 635.098 kb on - strand, within Shew_0560 at 635.362 kb on - strand, within Shew_0560 at 635.454 kb on + strand at 635.462 kb on - strand at 635.772 kb on + strand at 635.854 kb on + strand at 635.899 kb on - strand at 636.557 kb on - strand, within Shew_0561 at 636.638 kb on - strand, within Shew_0561 at 636.832 kb on - strand, within Shew_0561 at 636.863 kb on - strand, within Shew_0561 at 636.863 kb on - strand, within Shew_0561 at 636.868 kb on - strand, within Shew_0561 at 637.105 kb on + strand, within Shew_0561 at 637.369 kb on + strand at 637.369 kb on + strand at 637.377 kb on - strand at 637.377 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 635,031 + +0.5 635,049 - +0.3 635,088 - Shew_0560 0.18 -2.2 635,090 + Shew_0560 0.19 +0.2 635,090 + Shew_0560 0.19 +0.6 635,098 - Shew_0560 0.20 +1.6 635,362 - Shew_0560 0.78 +0.2 635,454 + +0.0 635,462 - +0.3 635,772 + -0.3 635,854 + +0.2 635,899 - +0.4 636,557 - Shew_0561 0.45 +0.1 636,638 - Shew_0561 0.50 -0.1 636,832 - Shew_0561 0.63 -1.0 636,863 - Shew_0561 0.65 +0.1 636,863 - Shew_0561 0.65 -0.1 636,868 - Shew_0561 0.66 +0.0 637,105 + Shew_0561 0.82 -0.3 637,369 + +0.2 637,369 + +0.4 637,377 - -0.1 637,377 - +0.6
Or see this region's nucleotide sequence