Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07175 and MMJJ_RS07180 are separated by 9 nucleotides MMJJ_RS07180 and MMJJ_RS07185 are separated by 2 nucleotides MMJJ_RS07185 and MMJJ_RS07190 are separated by 138 nucleotides
MMJJ_RS07175: MMJJ_RS07175 - DEAD/DEAH box helicase, at 1,312,177 to 1,314,444
_RS07175
MMJJ_RS07180: MMJJ_RS07180 - type I 3-dehydroquinate dehydratase, at 1,314,454 to 1,315,110
_RS07180
MMJJ_RS07185: MMJJ_RS07185 - DUF371 domain-containing protein, at 1,315,113 to 1,315,529
_RS07185
MMJJ_RS07190: MMJJ_RS07190 - RtcB family protein, at 1,315,668 to 1,317,110
_RS07190
Position (kb)
1314
1315
1316 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1313.459 kb on - strand, within MMJJ_RS07175 at 1313.460 kb on + strand, within MMJJ_RS07175 at 1313.465 kb on + strand, within MMJJ_RS07175 at 1313.538 kb on + strand, within MMJJ_RS07175 at 1313.546 kb on - strand, within MMJJ_RS07175 at 1313.622 kb on + strand, within MMJJ_RS07175 at 1313.637 kb on - strand, within MMJJ_RS07175 at 1313.638 kb on + strand, within MMJJ_RS07175 at 1313.642 kb on - strand, within MMJJ_RS07175 at 1313.642 kb on - strand, within MMJJ_RS07175 at 1313.643 kb on + strand, within MMJJ_RS07175 at 1313.663 kb on - strand, within MMJJ_RS07175 at 1313.709 kb on - strand, within MMJJ_RS07175 at 1313.710 kb on + strand, within MMJJ_RS07175 at 1313.710 kb on + strand, within MMJJ_RS07175 at 1313.713 kb on + strand, within MMJJ_RS07175 at 1313.722 kb on - strand, within MMJJ_RS07175 at 1313.722 kb on - strand, within MMJJ_RS07175 at 1313.723 kb on + strand, within MMJJ_RS07175 at 1313.732 kb on - strand, within MMJJ_RS07175 at 1313.733 kb on + strand, within MMJJ_RS07175 at 1313.744 kb on - strand, within MMJJ_RS07175 at 1313.744 kb on - strand, within MMJJ_RS07175 at 1313.744 kb on - strand, within MMJJ_RS07175 at 1313.745 kb on + strand, within MMJJ_RS07175 at 1313.745 kb on + strand, within MMJJ_RS07175 at 1313.752 kb on + strand, within MMJJ_RS07175 at 1313.763 kb on - strand, within MMJJ_RS07175 at 1313.768 kb on - strand, within MMJJ_RS07175 at 1313.768 kb on - strand, within MMJJ_RS07175 at 1313.769 kb on + strand, within MMJJ_RS07175 at 1313.769 kb on + strand, within MMJJ_RS07175 at 1313.805 kb on - strand, within MMJJ_RS07175 at 1313.806 kb on + strand, within MMJJ_RS07175 at 1313.834 kb on - strand, within MMJJ_RS07175 at 1313.835 kb on + strand, within MMJJ_RS07175 at 1313.835 kb on + strand, within MMJJ_RS07175 at 1313.837 kb on - strand, within MMJJ_RS07175 at 1313.859 kb on - strand, within MMJJ_RS07175 at 1313.869 kb on - strand, within MMJJ_RS07175 at 1313.923 kb on + strand, within MMJJ_RS07175 at 1313.928 kb on + strand, within MMJJ_RS07175 at 1314.000 kb on - strand, within MMJJ_RS07175 at 1314.008 kb on - strand, within MMJJ_RS07175 at 1314.017 kb on - strand, within MMJJ_RS07175 at 1314.066 kb on - strand, within MMJJ_RS07175 at 1314.125 kb on - strand, within MMJJ_RS07175 at 1314.132 kb on + strand, within MMJJ_RS07175 at 1314.156 kb on - strand, within MMJJ_RS07175 at 1314.156 kb on - strand, within MMJJ_RS07175 at 1314.157 kb on + strand, within MMJJ_RS07175 at 1314.216 kb on + strand, within MMJJ_RS07175 at 1314.216 kb on + strand, within MMJJ_RS07175 at 1314.219 kb on - strand at 1314.219 kb on - strand at 1314.220 kb on + strand at 1314.220 kb on + strand at 1314.220 kb on + strand at 1314.221 kb on - strand at 1314.249 kb on + strand at 1314.273 kb on + strand at 1314.328 kb on + strand at 1314.344 kb on - strand at 1314.345 kb on + strand at 1314.345 kb on + strand at 1314.349 kb on + strand at 1314.403 kb on + strand at 1314.452 kb on + strand at 1315.103 kb on - strand at 1315.108 kb on - strand at 1315.109 kb on + strand at 1315.224 kb on + strand, within MMJJ_RS07185 at 1315.391 kb on - strand, within MMJJ_RS07185 at 1315.392 kb on + strand, within MMJJ_RS07185 at 1315.392 kb on + strand, within MMJJ_RS07185 at 1315.454 kb on - strand, within MMJJ_RS07185 at 1315.530 kb on - strand at 1315.566 kb on - strand at 1315.566 kb on - strand at 1315.567 kb on + strand at 1315.576 kb on - strand at 1315.592 kb on - strand at 1315.596 kb on - strand at 1315.596 kb on - strand at 1315.603 kb on + strand at 1315.603 kb on + strand at 1315.611 kb on + strand at 1315.611 kb on + strand at 1315.614 kb on - strand at 1315.614 kb on - strand at 1315.615 kb on + strand at 1315.615 kb on + strand at 1315.618 kb on - strand at 1315.621 kb on + strand at 1315.796 kb on + strand at 1315.799 kb on - strand at 1315.925 kb on + strand, within MMJJ_RS07190
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 1,313,459 - MMJJ_RS07175 0.57 +0.8 1,313,460 + MMJJ_RS07175 0.57 -1.3 1,313,465 + MMJJ_RS07175 0.57 +0.1 1,313,538 + MMJJ_RS07175 0.60 -0.1 1,313,546 - MMJJ_RS07175 0.60 +0.1 1,313,622 + MMJJ_RS07175 0.64 +1.9 1,313,637 - MMJJ_RS07175 0.64 -0.0 1,313,638 + MMJJ_RS07175 0.64 +0.6 1,313,642 - MMJJ_RS07175 0.65 -0.6 1,313,642 - MMJJ_RS07175 0.65 -0.9 1,313,643 + MMJJ_RS07175 0.65 -0.5 1,313,663 - MMJJ_RS07175 0.66 -1.2 1,313,709 - MMJJ_RS07175 0.68 +1.2 1,313,710 + MMJJ_RS07175 0.68 +0.6 1,313,710 + MMJJ_RS07175 0.68 +0.0 1,313,713 + MMJJ_RS07175 0.68 -0.5 1,313,722 - MMJJ_RS07175 0.68 +0.5 1,313,722 - MMJJ_RS07175 0.68 +0.2 1,313,723 + MMJJ_RS07175 0.68 +1.7 1,313,732 - MMJJ_RS07175 0.69 -0.0 1,313,733 + MMJJ_RS07175 0.69 +0.9 1,313,744 - MMJJ_RS07175 0.69 +1.4 1,313,744 - MMJJ_RS07175 0.69 +1.8 1,313,744 - MMJJ_RS07175 0.69 +0.3 1,313,745 + MMJJ_RS07175 0.69 +2.0 1,313,745 + MMJJ_RS07175 0.69 +0.6 1,313,752 + MMJJ_RS07175 0.69 -0.6 1,313,763 - MMJJ_RS07175 0.70 +0.5 1,313,768 - MMJJ_RS07175 0.70 -0.4 1,313,768 - MMJJ_RS07175 0.70 -0.5 1,313,769 + MMJJ_RS07175 0.70 -0.7 1,313,769 + MMJJ_RS07175 0.70 -0.2 1,313,805 - MMJJ_RS07175 0.72 +1.9 1,313,806 + MMJJ_RS07175 0.72 -0.3 1,313,834 - MMJJ_RS07175 0.73 +0.7 1,313,835 + MMJJ_RS07175 0.73 -0.4 1,313,835 + MMJJ_RS07175 0.73 +0.3 1,313,837 - MMJJ_RS07175 0.73 -0.5 1,313,859 - MMJJ_RS07175 0.74 -2.0 1,313,869 - MMJJ_RS07175 0.75 -0.3 1,313,923 + MMJJ_RS07175 0.77 -0.0 1,313,928 + MMJJ_RS07175 0.77 -0.9 1,314,000 - MMJJ_RS07175 0.80 -2.0 1,314,008 - MMJJ_RS07175 0.81 -0.0 1,314,017 - MMJJ_RS07175 0.81 +0.5 1,314,066 - MMJJ_RS07175 0.83 -0.8 1,314,125 - MMJJ_RS07175 0.86 -1.0 1,314,132 + MMJJ_RS07175 0.86 +0.3 1,314,156 - MMJJ_RS07175 0.87 +0.3 1,314,156 - MMJJ_RS07175 0.87 +2.3 1,314,157 + MMJJ_RS07175 0.87 +0.9 1,314,216 + MMJJ_RS07175 0.90 -0.3 1,314,216 + MMJJ_RS07175 0.90 +0.2 1,314,219 - -3.2 1,314,219 - -1.5 1,314,220 + -0.2 1,314,220 + +2.1 1,314,220 + -0.2 1,314,221 - -2.2 1,314,249 + +2.3 1,314,273 + +1.0 1,314,328 + -1.4 1,314,344 - -1.7 1,314,345 + +0.3 1,314,345 + +2.2 1,314,349 + +0.1 1,314,403 + -1.2 1,314,452 + +0.5 1,315,103 - +0.3 1,315,108 - +0.1 1,315,109 + +0.7 1,315,224 + MMJJ_RS07185 0.27 -0.0 1,315,391 - MMJJ_RS07185 0.67 -2.3 1,315,392 + MMJJ_RS07185 0.67 +1.2 1,315,392 + MMJJ_RS07185 0.67 +0.2 1,315,454 - MMJJ_RS07185 0.82 -0.0 1,315,530 - +0.6 1,315,566 - +2.1 1,315,566 - +0.1 1,315,567 + -1.0 1,315,576 - -0.3 1,315,592 - -0.9 1,315,596 - -1.6 1,315,596 - -0.5 1,315,603 + -0.5 1,315,603 + +0.1 1,315,611 + -0.7 1,315,611 + +0.7 1,315,614 - -4.3 1,315,614 - -0.8 1,315,615 + -0.3 1,315,615 + -0.7 1,315,618 - -2.1 1,315,621 + -0.0 1,315,796 + -1.7 1,315,799 - -1.5 1,315,925 + MMJJ_RS07190 0.18 -2.7
Or see this region's nucleotide sequence