Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06140 and MMJJ_RS06145 are separated by 30 nucleotides MMJJ_RS06145 and MMJJ_RS06150 are separated by 15 nucleotides MMJJ_RS06150 and MMJJ_RS06155 are separated by 82 nucleotides MMJJ_RS06155 and MMJJ_RS06160 are separated by 19 nucleotides
MMJJ_RS06140: MMJJ_RS06140 - RimK family alpha-L-glutamate ligase, at 1,139,781 to 1,140,587
_RS06140
MMJJ_RS06145: MMJJ_RS06145 - TrkA family potassium uptake protein, at 1,140,618 to 1,141,283
_RS06145
MMJJ_RS06150: MMJJ_RS06150 - DUF483 domain-containing protein, at 1,141,299 to 1,141,853
_RS06150
MMJJ_RS06155: MMJJ_RS06155 - phosphatidylglycerophosphatase A, at 1,141,936 to 1,142,424
_RS06155
MMJJ_RS06160: MMJJ_RS06160 - stage II sporulation protein M, at 1,142,444 to 1,143,034
_RS06160
Position (kb)
1141
1142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1140.357 kb on - strand, within MMJJ_RS06140 at 1140.358 kb on + strand, within MMJJ_RS06140 at 1140.387 kb on + strand, within MMJJ_RS06140 at 1140.391 kb on - strand, within MMJJ_RS06140 at 1140.463 kb on - strand, within MMJJ_RS06140 at 1140.466 kb on - strand, within MMJJ_RS06140 at 1140.466 kb on - strand, within MMJJ_RS06140 at 1140.487 kb on + strand, within MMJJ_RS06140 at 1140.494 kb on + strand, within MMJJ_RS06140 at 1140.538 kb on - strand at 1140.564 kb on - strand at 1140.565 kb on + strand at 1140.572 kb on - strand at 1140.572 kb on - strand at 1140.572 kb on - strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.576 kb on - strand at 1140.577 kb on + strand at 1140.580 kb on - strand at 1140.580 kb on - strand at 1140.580 kb on - strand at 1140.585 kb on - strand at 1140.643 kb on + strand at 1140.762 kb on + strand, within MMJJ_RS06145 at 1140.940 kb on + strand, within MMJJ_RS06145 at 1141.207 kb on + strand, within MMJJ_RS06145 at 1141.399 kb on - strand, within MMJJ_RS06150 at 1141.495 kb on - strand, within MMJJ_RS06150 at 1141.495 kb on - strand, within MMJJ_RS06150 at 1141.548 kb on - strand, within MMJJ_RS06150 at 1141.555 kb on - strand, within MMJJ_RS06150 at 1141.640 kb on + strand, within MMJJ_RS06150 at 1141.671 kb on - strand, within MMJJ_RS06150 at 1141.739 kb on - strand, within MMJJ_RS06150 at 1141.741 kb on - strand, within MMJJ_RS06150 at 1141.742 kb on + strand, within MMJJ_RS06150 at 1141.815 kb on - strand at 1141.815 kb on - strand at 1141.825 kb on + strand at 1141.835 kb on + strand at 1141.884 kb on + strand at 1141.884 kb on + strand at 1141.885 kb on - strand at 1141.885 kb on - strand at 1141.885 kb on - strand at 1141.885 kb on - strand at 1141.885 kb on - strand at 1141.886 kb on + strand at 1141.886 kb on + strand at 1141.886 kb on + strand at 1141.887 kb on - strand at 1141.887 kb on - strand at 1141.887 kb on - strand at 1141.887 kb on - strand at 1141.887 kb on - strand at 1141.888 kb on + strand at 1141.888 kb on + strand at 1141.888 kb on + strand at 1141.890 kb on - strand at 1141.890 kb on - strand at 1141.890 kb on - strand at 1141.890 kb on - strand at 1141.896 kb on - strand at 1141.897 kb on + strand at 1141.897 kb on + strand at 1141.897 kb on + strand at 1141.902 kb on - strand at 1141.902 kb on - strand at 1141.902 kb on - strand at 1141.902 kb on - strand at 1141.903 kb on + strand at 1141.903 kb on + strand at 1141.903 kb on + strand at 1141.903 kb on + strand at 1141.903 kb on + strand at 1141.905 kb on - strand at 1141.915 kb on - strand at 1141.930 kb on + strand at 1141.937 kb on - strand at 1141.938 kb on + strand at 1142.000 kb on - strand, within MMJJ_RS06155 at 1142.001 kb on + strand, within MMJJ_RS06155 at 1142.026 kb on - strand, within MMJJ_RS06155 at 1142.027 kb on + strand, within MMJJ_RS06155 at 1142.108 kb on + strand, within MMJJ_RS06155 at 1142.109 kb on - strand, within MMJJ_RS06155 at 1142.130 kb on + strand, within MMJJ_RS06155 at 1142.191 kb on - strand, within MMJJ_RS06155 at 1142.192 kb on + strand, within MMJJ_RS06155 at 1142.192 kb on + strand, within MMJJ_RS06155 at 1142.211 kb on - strand, within MMJJ_RS06155 at 1142.266 kb on - strand, within MMJJ_RS06155 at 1142.295 kb on - strand, within MMJJ_RS06155 at 1142.303 kb on - strand at 1142.304 kb on + strand, within MMJJ_RS06155 at 1142.304 kb on + strand, within MMJJ_RS06155 at 1142.487 kb on - strand at 1142.498 kb on + strand at 1142.557 kb on + strand, within MMJJ_RS06160 at 1142.560 kb on - strand at 1142.561 kb on + strand, within MMJJ_RS06160 at 1142.568 kb on + strand, within MMJJ_RS06160 at 1142.606 kb on - strand, within MMJJ_RS06160 at 1142.650 kb on - strand, within MMJJ_RS06160 at 1142.690 kb on + strand, within MMJJ_RS06160 at 1142.710 kb on - strand, within MMJJ_RS06160 at 1142.710 kb on - strand, within MMJJ_RS06160 at 1142.714 kb on - strand, within MMJJ_RS06160 at 1142.714 kb on - strand, within MMJJ_RS06160 at 1142.714 kb on - strand, within MMJJ_RS06160 at 1142.714 kb on - strand, within MMJJ_RS06160 at 1142.714 kb on - strand, within MMJJ_RS06160 at 1142.715 kb on + strand, within MMJJ_RS06160 at 1142.715 kb on + strand, within MMJJ_RS06160 at 1142.715 kb on + strand, within MMJJ_RS06160 at 1142.715 kb on + strand, within MMJJ_RS06160 at 1142.715 kb on + strand, within MMJJ_RS06160 at 1142.716 kb on - strand, within MMJJ_RS06160 at 1142.716 kb on - strand, within MMJJ_RS06160 at 1142.717 kb on + strand, within MMJJ_RS06160 at 1142.717 kb on + strand, within MMJJ_RS06160 at 1142.717 kb on + strand, within MMJJ_RS06160 at 1142.717 kb on + strand, within MMJJ_RS06160 at 1142.717 kb on + strand, within MMJJ_RS06160 at 1142.717 kb on + strand, within MMJJ_RS06160 at 1142.717 kb on + strand, within MMJJ_RS06160 at 1142.717 kb on + strand, within MMJJ_RS06160 at 1142.722 kb on - strand, within MMJJ_RS06160 at 1142.723 kb on + strand, within MMJJ_RS06160 at 1142.723 kb on + strand, within MMJJ_RS06160 at 1142.723 kb on + strand, within MMJJ_RS06160 at 1142.723 kb on + strand, within MMJJ_RS06160 at 1142.737 kb on - strand, within MMJJ_RS06160 at 1142.740 kb on + strand, within MMJJ_RS06160 at 1142.740 kb on + strand, within MMJJ_RS06160 at 1142.740 kb on + strand, within MMJJ_RS06160 at 1142.740 kb on + strand, within MMJJ_RS06160 at 1142.752 kb on + strand, within MMJJ_RS06160 at 1142.772 kb on + strand, within MMJJ_RS06160 at 1142.812 kb on + strand, within MMJJ_RS06160
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 1,140,357 - MMJJ_RS06140 0.71 +0.7 1,140,358 + MMJJ_RS06140 0.71 +0.8 1,140,387 + MMJJ_RS06140 0.75 -0.5 1,140,391 - MMJJ_RS06140 0.76 -0.2 1,140,463 - MMJJ_RS06140 0.85 -0.1 1,140,466 - MMJJ_RS06140 0.85 -0.6 1,140,466 - MMJJ_RS06140 0.85 -0.2 1,140,487 + MMJJ_RS06140 0.87 -0.0 1,140,494 + MMJJ_RS06140 0.88 -0.1 1,140,538 - -0.7 1,140,564 - +0.7 1,140,565 + +1.9 1,140,572 - -0.1 1,140,572 - +0.1 1,140,572 - -0.7 1,140,573 + +2.2 1,140,573 + +1.3 1,140,573 + +1.2 1,140,573 + -1.3 1,140,573 + -1.0 1,140,573 + -0.3 1,140,573 + +0.4 1,140,573 + -0.4 1,140,576 - -0.1 1,140,577 + +1.5 1,140,580 - +3.1 1,140,580 - -0.1 1,140,580 - +0.3 1,140,585 - +0.8 1,140,643 + +0.0 1,140,762 + MMJJ_RS06145 0.22 -1.8 1,140,940 + MMJJ_RS06145 0.48 -2.3 1,141,207 + MMJJ_RS06145 0.88 -0.0 1,141,399 - MMJJ_RS06150 0.18 -1.2 1,141,495 - MMJJ_RS06150 0.35 -3.2 1,141,495 - MMJJ_RS06150 0.35 -2.8 1,141,548 - MMJJ_RS06150 0.45 -0.1 1,141,555 - MMJJ_RS06150 0.46 +0.3 1,141,640 + MMJJ_RS06150 0.61 -1.2 1,141,671 - MMJJ_RS06150 0.67 -0.0 1,141,739 - MMJJ_RS06150 0.79 +1.3 1,141,741 - MMJJ_RS06150 0.80 -0.9 1,141,742 + MMJJ_RS06150 0.80 -1.3 1,141,815 - +0.0 1,141,815 - +1.7 1,141,825 + +0.3 1,141,835 + -2.3 1,141,884 + -0.5 1,141,884 + -1.9 1,141,885 - +0.2 1,141,885 - -1.3 1,141,885 - +0.6 1,141,885 - -0.6 1,141,885 - -0.6 1,141,886 + -1.9 1,141,886 + -1.5 1,141,886 + -2.4 1,141,887 - +2.1 1,141,887 - -1.1 1,141,887 - +1.4 1,141,887 - +0.5 1,141,887 - -0.2 1,141,888 + -2.3 1,141,888 + -3.1 1,141,888 + -1.9 1,141,890 - +0.3 1,141,890 - +0.5 1,141,890 - +0.1 1,141,890 - -0.6 1,141,896 - +1.5 1,141,897 + -3.0 1,141,897 + -2.1 1,141,897 + -0.6 1,141,902 - -0.1 1,141,902 - +0.7 1,141,902 - -0.7 1,141,902 - -0.9 1,141,903 + -2.4 1,141,903 + -2.4 1,141,903 + -1.6 1,141,903 + -2.2 1,141,903 + -0.8 1,141,905 - +0.2 1,141,915 - -0.5 1,141,930 + -0.5 1,141,937 - +0.6 1,141,938 + +0.6 1,142,000 - MMJJ_RS06155 0.13 -2.5 1,142,001 + MMJJ_RS06155 0.13 -2.5 1,142,026 - MMJJ_RS06155 0.18 -2.3 1,142,027 + MMJJ_RS06155 0.19 -1.3 1,142,108 + MMJJ_RS06155 0.35 -2.9 1,142,109 - MMJJ_RS06155 0.35 -2.2 1,142,130 + MMJJ_RS06155 0.40 -1.8 1,142,191 - MMJJ_RS06155 0.52 -2.9 1,142,192 + MMJJ_RS06155 0.52 -2.4 1,142,192 + MMJJ_RS06155 0.52 -2.0 1,142,211 - MMJJ_RS06155 0.56 -2.4 1,142,266 - MMJJ_RS06155 0.67 -2.5 1,142,295 - MMJJ_RS06155 0.73 -1.8 1,142,303 - +1.5 1,142,304 + MMJJ_RS06155 0.75 -3.1 1,142,304 + MMJJ_RS06155 0.75 -1.9 1,142,487 - +0.2 1,142,498 + -0.5 1,142,557 + MMJJ_RS06160 0.19 +0.2 1,142,560 - +1.5 1,142,561 + MMJJ_RS06160 0.20 +0.8 1,142,568 + MMJJ_RS06160 0.21 -0.1 1,142,606 - MMJJ_RS06160 0.27 +0.8 1,142,650 - MMJJ_RS06160 0.35 +0.1 1,142,690 + MMJJ_RS06160 0.42 -0.8 1,142,710 - MMJJ_RS06160 0.45 +0.8 1,142,710 - MMJJ_RS06160 0.45 +2.2 1,142,714 - MMJJ_RS06160 0.46 +0.3 1,142,714 - MMJJ_RS06160 0.46 -0.1 1,142,714 - MMJJ_RS06160 0.46 +0.1 1,142,714 - MMJJ_RS06160 0.46 +1.1 1,142,714 - MMJJ_RS06160 0.46 +0.6 1,142,715 + MMJJ_RS06160 0.46 -1.6 1,142,715 + MMJJ_RS06160 0.46 -0.1 1,142,715 + MMJJ_RS06160 0.46 +2.0 1,142,715 + MMJJ_RS06160 0.46 +1.2 1,142,715 + MMJJ_RS06160 0.46 +1.5 1,142,716 - MMJJ_RS06160 0.46 +0.7 1,142,716 - MMJJ_RS06160 0.46 +0.5 1,142,717 + MMJJ_RS06160 0.46 +2.4 1,142,717 + MMJJ_RS06160 0.46 -0.3 1,142,717 + MMJJ_RS06160 0.46 +0.6 1,142,717 + MMJJ_RS06160 0.46 -0.3 1,142,717 + MMJJ_RS06160 0.46 -0.1 1,142,717 + MMJJ_RS06160 0.46 +0.3 1,142,717 + MMJJ_RS06160 0.46 +3.3 1,142,717 + MMJJ_RS06160 0.46 -0.2 1,142,722 - MMJJ_RS06160 0.47 +1.6 1,142,723 + MMJJ_RS06160 0.47 +1.1 1,142,723 + MMJJ_RS06160 0.47 +0.2 1,142,723 + MMJJ_RS06160 0.47 +0.1 1,142,723 + MMJJ_RS06160 0.47 -0.3 1,142,737 - MMJJ_RS06160 0.50 +0.4 1,142,740 + MMJJ_RS06160 0.50 +0.8 1,142,740 + MMJJ_RS06160 0.50 +0.7 1,142,740 + MMJJ_RS06160 0.50 -0.6 1,142,740 + MMJJ_RS06160 0.50 +0.5 1,142,752 + MMJJ_RS06160 0.52 +0.6 1,142,772 + MMJJ_RS06160 0.55 -0.6 1,142,812 + MMJJ_RS06160 0.62 +2.6
Or see this region's nucleotide sequence