Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05375 and MMJJ_RS05380 are separated by 121 nucleotides MMJJ_RS05380 and MMJJ_RS05385 are separated by 14 nucleotides MMJJ_RS05385 and MMJJ_RS05390 are separated by 11 nucleotides
MMJJ_RS05375: MMJJ_RS05375 - radical SAM protein, at 997,919 to 998,971
_RS05375
MMJJ_RS05380: MMJJ_RS05380 - hypothetical protein, at 999,093 to 999,584
_RS05380
MMJJ_RS05385: MMJJ_RS05385 - hypothetical protein, at 999,599 to 1,000,402
_RS05385
MMJJ_RS05390: MMJJ_RS05390 - tRNA pseudouridine(13) synthase TruD, at 1,000,414 to 1,001,583
_RS05390
Position (kb) 999
1000 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 998.134 kb on - strand, within MMJJ_RS05375 at 998.135 kb on + strand, within MMJJ_RS05375 at 998.135 kb on + strand, within MMJJ_RS05375 at 998.135 kb on + strand, within MMJJ_RS05375 at 998.139 kb on - strand, within MMJJ_RS05375 at 998.190 kb on + strand, within MMJJ_RS05375 at 998.205 kb on - strand, within MMJJ_RS05375 at 998.205 kb on - strand, within MMJJ_RS05375 at 998.213 kb on + strand, within MMJJ_RS05375 at 998.231 kb on - strand, within MMJJ_RS05375 at 998.232 kb on + strand, within MMJJ_RS05375 at 998.253 kb on + strand, within MMJJ_RS05375 at 998.257 kb on + strand, within MMJJ_RS05375 at 998.257 kb on + strand, within MMJJ_RS05375 at 998.257 kb on + strand, within MMJJ_RS05375 at 998.257 kb on + strand, within MMJJ_RS05375 at 998.300 kb on + strand, within MMJJ_RS05375 at 998.331 kb on - strand, within MMJJ_RS05375 at 998.431 kb on - strand, within MMJJ_RS05375 at 998.432 kb on + strand, within MMJJ_RS05375 at 998.432 kb on + strand, within MMJJ_RS05375 at 998.434 kb on - strand, within MMJJ_RS05375 at 998.434 kb on - strand, within MMJJ_RS05375 at 998.434 kb on - strand, within MMJJ_RS05375 at 998.436 kb on - strand, within MMJJ_RS05375 at 998.442 kb on - strand, within MMJJ_RS05375 at 998.469 kb on - strand, within MMJJ_RS05375 at 998.470 kb on + strand, within MMJJ_RS05375 at 998.482 kb on + strand, within MMJJ_RS05375 at 998.499 kb on - strand, within MMJJ_RS05375 at 998.500 kb on + strand, within MMJJ_RS05375 at 998.515 kb on - strand, within MMJJ_RS05375 at 998.516 kb on + strand, within MMJJ_RS05375 at 998.516 kb on + strand, within MMJJ_RS05375 at 998.585 kb on - strand, within MMJJ_RS05375 at 998.585 kb on - strand, within MMJJ_RS05375 at 998.588 kb on + strand, within MMJJ_RS05375 at 998.634 kb on - strand, within MMJJ_RS05375 at 998.701 kb on + strand, within MMJJ_RS05375 at 998.701 kb on + strand, within MMJJ_RS05375 at 998.704 kb on - strand, within MMJJ_RS05375 at 998.735 kb on + strand, within MMJJ_RS05375 at 998.788 kb on + strand, within MMJJ_RS05375 at 998.788 kb on + strand, within MMJJ_RS05375 at 998.788 kb on + strand, within MMJJ_RS05375 at 998.789 kb on - strand at 998.790 kb on + strand, within MMJJ_RS05375 at 998.849 kb on - strand, within MMJJ_RS05375 at 998.849 kb on - strand, within MMJJ_RS05375 at 998.849 kb on - strand, within MMJJ_RS05375 at 998.849 kb on - strand, within MMJJ_RS05375 at 998.849 kb on - strand, within MMJJ_RS05375 at 998.849 kb on - strand, within MMJJ_RS05375 at 998.850 kb on + strand, within MMJJ_RS05375 at 998.850 kb on + strand, within MMJJ_RS05375 at 998.850 kb on + strand, within MMJJ_RS05375 at 998.850 kb on + strand, within MMJJ_RS05375 at 998.850 kb on + strand, within MMJJ_RS05375 at 998.854 kb on + strand, within MMJJ_RS05375 at 998.870 kb on + strand at 998.893 kb on - strand at 998.894 kb on + strand at 998.896 kb on + strand at 998.928 kb on - strand at 998.937 kb on + strand at 998.937 kb on + strand at 998.937 kb on + strand at 998.941 kb on - strand at 998.941 kb on - strand at 998.953 kb on - strand at 998.972 kb on - strand at 998.973 kb on + strand at 998.974 kb on - strand at 998.974 kb on - strand at 998.974 kb on - strand at 998.974 kb on - strand at 998.975 kb on + strand at 998.975 kb on + strand at 998.975 kb on + strand at 998.975 kb on + strand at 998.975 kb on + strand at 998.975 kb on + strand at 998.975 kb on + strand at 998.975 kb on + strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.977 kb on - strand at 998.978 kb on + strand at 998.978 kb on + strand at 998.978 kb on + strand at 998.978 kb on + strand at 998.978 kb on + strand at 998.978 kb on + strand at 998.978 kb on + strand at 998.978 kb on + strand at 998.978 kb on + strand at 998.979 kb on - strand at 998.979 kb on - strand at 998.980 kb on + strand at 998.980 kb on + strand at 998.985 kb on - strand at 998.985 kb on - strand at 998.986 kb on + strand at 998.990 kb on - strand at 998.990 kb on - strand at 998.995 kb on - strand at 998.995 kb on - strand at 998.996 kb on + strand at 999.009 kb on - strand at 999.010 kb on + strand at 999.015 kb on + strand at 999.038 kb on - strand at 999.039 kb on + strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.040 kb on - strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.041 kb on + strand at 999.042 kb on - strand at 999.042 kb on - strand at 999.042 kb on - strand at 999.042 kb on - strand at 999.042 kb on - strand at 999.042 kb on - strand at 999.042 kb on - strand at 999.042 kb on - strand at 999.042 kb on - strand at 999.042 kb on - strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.043 kb on + strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.044 kb on - strand at 999.045 kb on + strand at 999.045 kb on + strand at 999.045 kb on + strand at 999.045 kb on + strand at 999.057 kb on - strand at 999.057 kb on - strand at 999.058 kb on + strand at 999.058 kb on + strand at 999.058 kb on + strand at 999.062 kb on + strand at 999.062 kb on + strand at 999.076 kb on + strand at 999.089 kb on - strand at 999.092 kb on - strand at 999.093 kb on + strand at 999.290 kb on + strand, within MMJJ_RS05380 at 999.461 kb on + strand, within MMJJ_RS05380 at 999.565 kb on + strand at 999.574 kb on + strand at 999.596 kb on + strand at 999.616 kb on - strand at 999.616 kb on - strand at 999.616 kb on - strand at 999.617 kb on + strand at 999.617 kb on + strand at 999.619 kb on + strand at 999.619 kb on + strand at 999.623 kb on + strand at 999.627 kb on - strand at 999.649 kb on - strand at 999.702 kb on - strand, within MMJJ_RS05385 at 999.726 kb on - strand, within MMJJ_RS05385 at 999.726 kb on - strand, within MMJJ_RS05385 at 999.727 kb on + strand, within MMJJ_RS05385 at 999.727 kb on + strand, within MMJJ_RS05385 at 999.740 kb on - strand at 999.745 kb on - strand, within MMJJ_RS05385 at 999.746 kb on + strand, within MMJJ_RS05385 at 999.746 kb on + strand, within MMJJ_RS05385 at 999.778 kb on - strand, within MMJJ_RS05385 at 999.778 kb on - strand, within MMJJ_RS05385 at 999.779 kb on + strand, within MMJJ_RS05385 at 999.804 kb on + strand, within MMJJ_RS05385 at 999.812 kb on + strand, within MMJJ_RS05385 at 999.846 kb on - strand, within MMJJ_RS05385 at 999.846 kb on - strand, within MMJJ_RS05385 at 999.847 kb on + strand, within MMJJ_RS05385 at 999.847 kb on + strand, within MMJJ_RS05385 at 999.847 kb on + strand, within MMJJ_RS05385 at 999.852 kb on - strand, within MMJJ_RS05385 at 999.852 kb on - strand, within MMJJ_RS05385 at 999.852 kb on - strand, within MMJJ_RS05385 at 999.855 kb on - strand, within MMJJ_RS05385 at 999.855 kb on - strand, within MMJJ_RS05385 at 999.856 kb on + strand, within MMJJ_RS05385 at 999.856 kb on + strand, within MMJJ_RS05385 at 999.856 kb on + strand, within MMJJ_RS05385 at 999.944 kb on - strand, within MMJJ_RS05385 at 999.945 kb on + strand, within MMJJ_RS05385 at 1000.038 kb on - strand, within MMJJ_RS05385 at 1000.039 kb on + strand, within MMJJ_RS05385 at 1000.088 kb on - strand, within MMJJ_RS05385 at 1000.098 kb on - strand, within MMJJ_RS05385 at 1000.098 kb on - strand, within MMJJ_RS05385 at 1000.098 kb on - strand, within MMJJ_RS05385 at 1000.099 kb on + strand, within MMJJ_RS05385 at 1000.099 kb on + strand, within MMJJ_RS05385 at 1000.099 kb on + strand, within MMJJ_RS05385 at 1000.110 kb on - strand, within MMJJ_RS05385 at 1000.159 kb on + strand, within MMJJ_RS05385 at 1000.159 kb on + strand, within MMJJ_RS05385 at 1000.159 kb on + strand, within MMJJ_RS05385 at 1000.210 kb on - strand, within MMJJ_RS05385 at 1000.217 kb on - strand, within MMJJ_RS05385 at 1000.278 kb on + strand, within MMJJ_RS05385 at 1000.278 kb on + strand, within MMJJ_RS05385 at 1000.348 kb on + strand at 1000.404 kb on - strand at 1000.405 kb on + strand at 1000.411 kb on + strand at 1000.411 kb on + strand at 1000.448 kb on - strand at 1000.570 kb on + strand, within MMJJ_RS05390
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 998,134 - MMJJ_RS05375 0.20 -1.9 998,135 + MMJJ_RS05375 0.21 -1.2 998,135 + MMJJ_RS05375 0.21 -2.2 998,135 + MMJJ_RS05375 0.21 -4.3 998,139 - MMJJ_RS05375 0.21 -1.4 998,190 + MMJJ_RS05375 0.26 -4.3 998,205 - MMJJ_RS05375 0.27 -5.3 998,205 - MMJJ_RS05375 0.27 -2.6 998,213 + MMJJ_RS05375 0.28 -1.0 998,231 - MMJJ_RS05375 0.30 -2.4 998,232 + MMJJ_RS05375 0.30 -3.3 998,253 + MMJJ_RS05375 0.32 -4.2 998,257 + MMJJ_RS05375 0.32 -2.0 998,257 + MMJJ_RS05375 0.32 -1.4 998,257 + MMJJ_RS05375 0.32 -2.1 998,257 + MMJJ_RS05375 0.32 -2.0 998,300 + MMJJ_RS05375 0.36 -3.1 998,331 - MMJJ_RS05375 0.39 -0.7 998,431 - MMJJ_RS05375 0.49 -1.5 998,432 + MMJJ_RS05375 0.49 -4.0 998,432 + MMJJ_RS05375 0.49 -1.7 998,434 - MMJJ_RS05375 0.49 -2.0 998,434 - MMJJ_RS05375 0.49 -1.8 998,434 - MMJJ_RS05375 0.49 -3.5 998,436 - MMJJ_RS05375 0.49 -2.7 998,442 - MMJJ_RS05375 0.50 -1.9 998,469 - MMJJ_RS05375 0.52 -1.2 998,470 + MMJJ_RS05375 0.52 +1.0 998,482 + MMJJ_RS05375 0.53 -1.0 998,499 - MMJJ_RS05375 0.55 -2.9 998,500 + MMJJ_RS05375 0.55 -2.2 998,515 - MMJJ_RS05375 0.57 -4.0 998,516 + MMJJ_RS05375 0.57 +0.5 998,516 + MMJJ_RS05375 0.57 -0.9 998,585 - MMJJ_RS05375 0.63 -1.1 998,585 - MMJJ_RS05375 0.63 -2.5 998,588 + MMJJ_RS05375 0.64 -4.8 998,634 - MMJJ_RS05375 0.68 -3.6 998,701 + MMJJ_RS05375 0.74 -1.6 998,701 + MMJJ_RS05375 0.74 -1.8 998,704 - MMJJ_RS05375 0.75 -0.8 998,735 + MMJJ_RS05375 0.77 -2.1 998,788 + MMJJ_RS05375 0.83 -3.7 998,788 + MMJJ_RS05375 0.83 -1.3 998,788 + MMJJ_RS05375 0.83 -2.1 998,789 - +1.3 998,790 + MMJJ_RS05375 0.83 -4.9 998,849 - MMJJ_RS05375 0.88 -5.1 998,849 - MMJJ_RS05375 0.88 -4.1 998,849 - MMJJ_RS05375 0.88 -1.0 998,849 - MMJJ_RS05375 0.88 -2.5 998,849 - MMJJ_RS05375 0.88 -1.3 998,849 - MMJJ_RS05375 0.88 -3.7 998,850 + MMJJ_RS05375 0.88 -2.2 998,850 + MMJJ_RS05375 0.88 -1.6 998,850 + MMJJ_RS05375 0.88 -2.4 998,850 + MMJJ_RS05375 0.88 -1.5 998,850 + MMJJ_RS05375 0.88 -3.0 998,854 + MMJJ_RS05375 0.89 -4.2 998,870 + -3.7 998,893 - +1.3 998,894 + -0.7 998,896 + -1.3 998,928 - -2.2 998,937 + -2.0 998,937 + -0.3 998,937 + -2.2 998,941 - -0.1 998,941 - +0.3 998,953 - +0.4 998,972 - -0.2 998,973 + +0.4 998,974 - +1.1 998,974 - +0.1 998,974 - +0.0 998,974 - +1.3 998,975 + -1.1 998,975 + -0.4 998,975 + -0.1 998,975 + +0.5 998,975 + -0.5 998,975 + +0.1 998,975 + -0.4 998,975 + +1.7 998,977 - +2.3 998,977 - +1.3 998,977 - -0.1 998,977 - +0.0 998,977 - +0.8 998,977 - +0.1 998,977 - +0.9 998,977 - -0.3 998,977 - +0.1 998,977 - -0.3 998,977 - +1.2 998,977 - -1.1 998,977 - +0.8 998,978 + -0.8 998,978 + +1.6 998,978 + -0.4 998,978 + +0.6 998,978 + -1.1 998,978 + -0.3 998,978 + -0.5 998,978 + +0.4 998,978 + +2.9 998,979 - +0.4 998,979 - -0.6 998,980 + -0.7 998,980 + +0.9 998,985 - +0.1 998,985 - -0.7 998,986 + -0.2 998,990 - +2.1 998,990 - +0.4 998,995 - +0.4 998,995 - -0.5 998,996 + +0.3 999,009 - +0.4 999,010 + -0.4 999,015 + -0.0 999,038 - +0.2 999,039 + +1.1 999,040 - -0.1 999,040 - +0.4 999,040 - -0.0 999,040 - -1.6 999,040 - -1.3 999,040 - -0.3 999,040 - +0.6 999,040 - -0.1 999,040 - +0.9 999,040 - -0.7 999,040 - -0.4 999,040 - +1.5 999,041 + +0.1 999,041 + +1.3 999,041 + -0.1 999,041 + +1.1 999,041 + -0.9 999,041 + -2.3 999,041 + -0.3 999,041 + -0.1 999,041 + -0.1 999,041 + +2.4 999,041 + +2.1 999,041 + -0.8 999,041 + +2.2 999,042 - -0.3 999,042 - +0.7 999,042 - -0.9 999,042 - -1.5 999,042 - -1.0 999,042 - -0.4 999,042 - -1.0 999,042 - -0.8 999,042 - -1.6 999,042 - +1.0 999,043 + -0.2 999,043 + +2.5 999,043 + -0.4 999,043 + -0.5 999,043 + +0.3 999,043 + -1.1 999,043 + +0.4 999,043 + -0.1 999,043 + +0.1 999,043 + -1.1 999,043 + +0.2 999,043 + -0.4 999,043 + -0.4 999,044 - -1.2 999,044 - +0.0 999,044 - +0.1 999,044 - -1.7 999,044 - -0.4 999,044 - -0.5 999,044 - -1.3 999,044 - -0.2 999,044 - -0.4 999,044 - +0.0 999,044 - -0.5 999,044 - -1.5 999,044 - +1.0 999,045 + +0.1 999,045 + -0.0 999,045 + -0.5 999,045 + -0.6 999,057 - -0.0 999,057 - -1.6 999,058 + -0.9 999,058 + -0.9 999,058 + +0.7 999,062 + -1.3 999,062 + -1.0 999,076 + -3.3 999,089 - -0.7 999,092 - -2.4 999,093 + -2.6 999,290 + MMJJ_RS05380 0.40 -3.1 999,461 + MMJJ_RS05380 0.75 -2.8 999,565 + -3.6 999,574 + -1.6 999,596 + -0.5 999,616 - +0.0 999,616 - -0.0 999,616 - -2.3 999,617 + -0.1 999,617 + -0.6 999,619 + +0.2 999,619 + +0.5 999,623 + +0.3 999,627 - +0.2 999,649 - -0.2 999,702 - MMJJ_RS05385 0.13 -0.1 999,726 - MMJJ_RS05385 0.16 +0.7 999,726 - MMJJ_RS05385 0.16 +0.4 999,727 + MMJJ_RS05385 0.16 +0.3 999,727 + MMJJ_RS05385 0.16 +0.2 999,740 - +1.3 999,745 - MMJJ_RS05385 0.18 +0.2 999,746 + MMJJ_RS05385 0.18 +1.9 999,746 + MMJJ_RS05385 0.18 +0.4 999,778 - MMJJ_RS05385 0.22 +1.7 999,778 - MMJJ_RS05385 0.22 +0.4 999,779 + MMJJ_RS05385 0.22 +0.6 999,804 + MMJJ_RS05385 0.25 -0.4 999,812 + MMJJ_RS05385 0.26 +0.4 999,846 - MMJJ_RS05385 0.31 +0.3 999,846 - MMJJ_RS05385 0.31 -0.8 999,847 + MMJJ_RS05385 0.31 +0.2 999,847 + MMJJ_RS05385 0.31 +0.1 999,847 + MMJJ_RS05385 0.31 -0.2 999,852 - MMJJ_RS05385 0.31 -0.1 999,852 - MMJJ_RS05385 0.31 -0.6 999,852 - MMJJ_RS05385 0.31 +1.5 999,855 - MMJJ_RS05385 0.32 -0.3 999,855 - MMJJ_RS05385 0.32 +0.3 999,856 + MMJJ_RS05385 0.32 -0.2 999,856 + MMJJ_RS05385 0.32 +2.1 999,856 + MMJJ_RS05385 0.32 -0.5 999,944 - MMJJ_RS05385 0.43 +0.2 999,945 + MMJJ_RS05385 0.43 +1.3 1,000,038 - MMJJ_RS05385 0.55 -0.8 1,000,039 + MMJJ_RS05385 0.55 -0.3 1,000,088 - MMJJ_RS05385 0.61 +0.5 1,000,098 - MMJJ_RS05385 0.62 -0.5 1,000,098 - MMJJ_RS05385 0.62 -0.3 1,000,098 - MMJJ_RS05385 0.62 -0.4 1,000,099 + MMJJ_RS05385 0.62 +0.6 1,000,099 + MMJJ_RS05385 0.62 -0.1 1,000,099 + MMJJ_RS05385 0.62 -0.5 1,000,110 - MMJJ_RS05385 0.64 +0.4 1,000,159 + MMJJ_RS05385 0.70 +0.2 1,000,159 + MMJJ_RS05385 0.70 -1.5 1,000,159 + MMJJ_RS05385 0.70 +0.5 1,000,210 - MMJJ_RS05385 0.76 +0.5 1,000,217 - MMJJ_RS05385 0.77 -0.4 1,000,278 + MMJJ_RS05385 0.84 +0.4 1,000,278 + MMJJ_RS05385 0.84 -0.0 1,000,348 + +0.0 1,000,404 - -0.0 1,000,405 + +0.3 1,000,411 + +0.7 1,000,411 + -0.4 1,000,448 - -0.4 1,000,570 + MMJJ_RS05390 0.13 -0.6
Or see this region's nucleotide sequence