Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23920 and CA265_RS23925 are separated by 289 nucleotides CA265_RS23925 and CA265_RS23930 overlap by 23 nucleotides CA265_RS23930 and CA265_RS23935 are separated by 89 nucleotides CA265_RS23935 and CA265_RS23940 are separated by 97 nucleotides
CA265_RS23920: CA265_RS23920 - DNA primase, at 5,834,365 to 5,836,344
_RS23920
CA265_RS23925: CA265_RS23925 - endonuclease, at 5,836,634 to 5,836,999
_RS23925
CA265_RS23930: CA265_RS23930 - glutamine cyclotransferase, at 5,836,977 to 5,838,059
_RS23930
CA265_RS23935: CA265_RS23935 - hypothetical protein, at 5,838,149 to 5,838,679
_RS23935
CA265_RS23940: CA265_RS23940 - hypothetical protein, at 5,838,777 to 5,839,409
_RS23940
Position (kb)
5836
5837
5838
5839 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5836.394 kb on + strand at 5836.401 kb on + strand at 5836.409 kb on + strand at 5836.410 kb on - strand at 5836.411 kb on + strand at 5836.412 kb on - strand at 5836.412 kb on - strand at 5836.449 kb on - strand at 5836.455 kb on + strand at 5836.455 kb on + strand at 5836.474 kb on + strand at 5836.503 kb on + strand at 5836.503 kb on + strand at 5836.542 kb on - strand at 5836.569 kb on + strand at 5836.570 kb on - strand at 5836.574 kb on - strand at 5836.582 kb on - strand at 5836.653 kb on + strand at 5836.672 kb on + strand, within CA265_RS23925 at 5836.713 kb on + strand at 5836.730 kb on + strand, within CA265_RS23925 at 5836.731 kb on - strand, within CA265_RS23925 at 5836.731 kb on - strand, within CA265_RS23925 at 5836.786 kb on - strand, within CA265_RS23925 at 5836.801 kb on + strand, within CA265_RS23925 at 5836.881 kb on - strand, within CA265_RS23925 at 5836.882 kb on + strand, within CA265_RS23925 at 5836.891 kb on - strand, within CA265_RS23925 at 5836.971 kb on + strand at 5836.971 kb on + strand at 5837.067 kb on - strand at 5837.076 kb on + strand at 5837.076 kb on + strand at 5837.077 kb on - strand at 5837.077 kb on - strand at 5837.198 kb on + strand, within CA265_RS23930 at 5837.199 kb on - strand, within CA265_RS23930 at 5837.262 kb on - strand, within CA265_RS23930 at 5837.329 kb on + strand, within CA265_RS23930 at 5837.330 kb on - strand, within CA265_RS23930 at 5837.330 kb on - strand, within CA265_RS23930 at 5837.355 kb on + strand, within CA265_RS23930 at 5837.356 kb on - strand, within CA265_RS23930 at 5837.361 kb on + strand, within CA265_RS23930 at 5837.391 kb on - strand, within CA265_RS23930 at 5837.401 kb on - strand, within CA265_RS23930 at 5837.405 kb on - strand, within CA265_RS23930 at 5837.530 kb on - strand, within CA265_RS23930 at 5837.575 kb on + strand, within CA265_RS23930 at 5837.602 kb on + strand, within CA265_RS23930 at 5837.603 kb on - strand, within CA265_RS23930 at 5837.603 kb on - strand, within CA265_RS23930 at 5837.605 kb on - strand, within CA265_RS23930 at 5837.609 kb on + strand, within CA265_RS23930 at 5837.609 kb on + strand, within CA265_RS23930 at 5837.609 kb on + strand, within CA265_RS23930 at 5837.610 kb on - strand, within CA265_RS23930 at 5837.610 kb on - strand, within CA265_RS23930 at 5837.692 kb on + strand, within CA265_RS23930 at 5837.692 kb on + strand, within CA265_RS23930 at 5837.738 kb on + strand, within CA265_RS23930 at 5837.738 kb on + strand, within CA265_RS23930 at 5837.742 kb on + strand, within CA265_RS23930 at 5837.778 kb on - strand, within CA265_RS23930 at 5837.843 kb on + strand, within CA265_RS23930 at 5838.030 kb on - strand at 5838.030 kb on - strand at 5838.104 kb on + strand at 5838.104 kb on + strand at 5838.104 kb on + strand at 5838.104 kb on + strand at 5838.112 kb on + strand at 5838.116 kb on + strand at 5838.173 kb on + strand at 5838.174 kb on - strand at 5838.177 kb on + strand at 5838.177 kb on + strand at 5838.231 kb on + strand, within CA265_RS23935 at 5838.289 kb on + strand, within CA265_RS23935 at 5838.307 kb on - strand, within CA265_RS23935 at 5838.355 kb on + strand, within CA265_RS23935 at 5838.361 kb on + strand, within CA265_RS23935 at 5838.431 kb on - strand, within CA265_RS23935 at 5838.593 kb on + strand, within CA265_RS23935 at 5838.739 kb on - strand at 5838.770 kb on - strand at 5838.778 kb on + strand at 5838.785 kb on + strand at 5838.788 kb on - strand at 5838.789 kb on + strand at 5838.798 kb on + strand at 5838.825 kb on + strand at 5838.858 kb on + strand, within CA265_RS23940 at 5838.878 kb on + strand, within CA265_RS23940 at 5838.897 kb on - strand, within CA265_RS23940 at 5839.016 kb on + strand, within CA265_RS23940 at 5839.042 kb on + strand, within CA265_RS23940 at 5839.042 kb on + strand, within CA265_RS23940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 5,836,394 + +0.1 5,836,401 + -1.0 5,836,409 + -0.2 5,836,410 - -0.3 5,836,411 + -0.5 5,836,412 - -1.2 5,836,412 - +0.6 5,836,449 - -0.3 5,836,455 + -0.5 5,836,455 + -0.3 5,836,474 + +0.3 5,836,503 + -0.4 5,836,503 + -0.2 5,836,542 - -0.5 5,836,569 + +0.8 5,836,570 - +0.3 5,836,574 - +0.3 5,836,582 - +0.4 5,836,653 + +0.1 5,836,672 + CA265_RS23925 0.10 +0.8 5,836,713 + +0.3 5,836,730 + CA265_RS23925 0.26 -0.5 5,836,731 - CA265_RS23925 0.27 -0.6 5,836,731 - CA265_RS23925 0.27 +0.6 5,836,786 - CA265_RS23925 0.42 +0.3 5,836,801 + CA265_RS23925 0.46 -0.8 5,836,881 - CA265_RS23925 0.67 +0.1 5,836,882 + CA265_RS23925 0.68 -0.1 5,836,891 - CA265_RS23925 0.70 +0.8 5,836,971 + -0.7 5,836,971 + -0.7 5,837,067 - -0.2 5,837,076 + +0.2 5,837,076 + +0.6 5,837,077 - -1.6 5,837,077 - -0.7 5,837,198 + CA265_RS23930 0.20 -0.2 5,837,199 - CA265_RS23930 0.20 -0.8 5,837,262 - CA265_RS23930 0.26 -0.1 5,837,329 + CA265_RS23930 0.33 -0.5 5,837,330 - CA265_RS23930 0.33 -3.5 5,837,330 - CA265_RS23930 0.33 -0.3 5,837,355 + CA265_RS23930 0.35 +0.0 5,837,356 - CA265_RS23930 0.35 -2.3 5,837,361 + CA265_RS23930 0.35 -0.7 5,837,391 - CA265_RS23930 0.38 -0.6 5,837,401 - CA265_RS23930 0.39 -0.9 5,837,405 - CA265_RS23930 0.40 -1.3 5,837,530 - CA265_RS23930 0.51 -1.9 5,837,575 + CA265_RS23930 0.55 -0.3 5,837,602 + CA265_RS23930 0.58 -2.2 5,837,603 - CA265_RS23930 0.58 -0.6 5,837,603 - CA265_RS23930 0.58 -1.3 5,837,605 - CA265_RS23930 0.58 -0.3 5,837,609 + CA265_RS23930 0.58 -0.0 5,837,609 + CA265_RS23930 0.58 -2.6 5,837,609 + CA265_RS23930 0.58 -0.8 5,837,610 - CA265_RS23930 0.58 +0.0 5,837,610 - CA265_RS23930 0.58 -0.7 5,837,692 + CA265_RS23930 0.66 +0.1 5,837,692 + CA265_RS23930 0.66 -1.1 5,837,738 + CA265_RS23930 0.70 -0.7 5,837,738 + CA265_RS23930 0.70 -0.6 5,837,742 + CA265_RS23930 0.71 +0.8 5,837,778 - CA265_RS23930 0.74 -0.9 5,837,843 + CA265_RS23930 0.80 -1.6 5,838,030 - +0.2 5,838,030 - -0.9 5,838,104 + +0.6 5,838,104 + +0.5 5,838,104 + +0.2 5,838,104 + -0.9 5,838,112 + -0.0 5,838,116 + +1.1 5,838,173 + -1.2 5,838,174 - +0.2 5,838,177 + -0.4 5,838,177 + +0.5 5,838,231 + CA265_RS23935 0.15 +0.3 5,838,289 + CA265_RS23935 0.26 -0.7 5,838,307 - CA265_RS23935 0.30 -0.2 5,838,355 + CA265_RS23935 0.39 -1.2 5,838,361 + CA265_RS23935 0.40 +0.5 5,838,431 - CA265_RS23935 0.53 +0.8 5,838,593 + CA265_RS23935 0.84 +0.7 5,838,739 - +0.4 5,838,770 - -3.4 5,838,778 + -1.0 5,838,785 + +1.1 5,838,788 - -0.2 5,838,789 + +1.1 5,838,798 + +0.7 5,838,825 + -0.2 5,838,858 + CA265_RS23940 0.13 -0.0 5,838,878 + CA265_RS23940 0.16 +0.5 5,838,897 - CA265_RS23940 0.19 -0.7 5,839,016 + CA265_RS23940 0.38 -0.7 5,839,042 + CA265_RS23940 0.42 -0.2 5,839,042 + CA265_RS23940 0.42 -1.4
Or see this region's nucleotide sequence