Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22985 and CA265_RS22990 are separated by 280 nucleotides CA265_RS22990 and CA265_RS22995 overlap by 4 nucleotides
CA265_RS22985: CA265_RS22985 - helicase SNF2, at 5,623,882 to 5,626,773
_RS22985
CA265_RS22990: CA265_RS22990 - hypothetical protein, at 5,627,054 to 5,628,064
_RS22990
CA265_RS22995: CA265_RS22995 - hypothetical protein, at 5,628,061 to 5,631,591
_RS22995
Position (kb)
5627
5628
5629 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5626.098 kb on - strand, within CA265_RS22985 at 5626.101 kb on + strand, within CA265_RS22985 at 5626.129 kb on - strand, within CA265_RS22985 at 5626.137 kb on + strand, within CA265_RS22985 at 5626.170 kb on + strand, within CA265_RS22985 at 5626.192 kb on + strand, within CA265_RS22985 at 5626.192 kb on + strand, within CA265_RS22985 at 5626.193 kb on - strand, within CA265_RS22985 at 5626.264 kb on + strand, within CA265_RS22985 at 5626.394 kb on - strand, within CA265_RS22985 at 5626.397 kb on + strand, within CA265_RS22985 at 5626.397 kb on + strand, within CA265_RS22985 at 5626.532 kb on + strand at 5626.587 kb on - strand at 5626.594 kb on - strand at 5626.600 kb on + strand at 5626.712 kb on + strand at 5626.734 kb on - strand at 5626.744 kb on - strand at 5626.753 kb on - strand at 5626.758 kb on + strand at 5626.769 kb on - strand at 5626.800 kb on + strand at 5626.801 kb on - strand at 5626.824 kb on + strand at 5626.829 kb on + strand at 5626.840 kb on + strand at 5626.840 kb on + strand at 5626.909 kb on + strand at 5626.972 kb on - strand at 5626.973 kb on + strand at 5626.988 kb on + strand at 5627.005 kb on + strand at 5627.012 kb on - strand at 5627.052 kb on - strand at 5627.135 kb on + strand at 5627.138 kb on - strand at 5627.209 kb on - strand, within CA265_RS22990 at 5627.253 kb on - strand, within CA265_RS22990 at 5627.263 kb on - strand, within CA265_RS22990 at 5627.350 kb on - strand, within CA265_RS22990 at 5627.357 kb on + strand, within CA265_RS22990 at 5627.357 kb on + strand, within CA265_RS22990 at 5627.359 kb on + strand, within CA265_RS22990 at 5627.437 kb on - strand, within CA265_RS22990 at 5627.447 kb on + strand, within CA265_RS22990 at 5627.447 kb on + strand, within CA265_RS22990 at 5627.447 kb on + strand, within CA265_RS22990 at 5627.448 kb on - strand, within CA265_RS22990 at 5627.448 kb on - strand, within CA265_RS22990 at 5627.518 kb on + strand, within CA265_RS22990 at 5627.542 kb on - strand, within CA265_RS22990 at 5627.615 kb on - strand, within CA265_RS22990 at 5627.615 kb on - strand, within CA265_RS22990 at 5627.623 kb on - strand, within CA265_RS22990 at 5627.624 kb on + strand, within CA265_RS22990 at 5627.625 kb on - strand, within CA265_RS22990 at 5627.625 kb on - strand, within CA265_RS22990 at 5627.626 kb on + strand, within CA265_RS22990 at 5627.626 kb on + strand, within CA265_RS22990 at 5627.750 kb on - strand, within CA265_RS22990 at 5627.750 kb on - strand, within CA265_RS22990 at 5627.917 kb on + strand, within CA265_RS22990 at 5627.918 kb on - strand, within CA265_RS22990 at 5627.932 kb on - strand, within CA265_RS22990 at 5627.945 kb on + strand, within CA265_RS22990 at 5627.945 kb on + strand, within CA265_RS22990 at 5627.952 kb on - strand, within CA265_RS22990 at 5627.959 kb on - strand, within CA265_RS22990 at 5627.982 kb on + strand at 5628.013 kb on + strand at 5628.022 kb on - strand at 5628.079 kb on - strand at 5628.122 kb on + strand at 5628.123 kb on - strand at 5628.126 kb on - strand at 5628.126 kb on - strand at 5628.182 kb on + strand at 5628.200 kb on + strand at 5628.225 kb on - strand at 5628.285 kb on + strand at 5628.285 kb on + strand at 5628.315 kb on + strand at 5628.350 kb on - strand at 5628.350 kb on - strand at 5628.426 kb on + strand, within CA265_RS22995 at 5628.511 kb on + strand, within CA265_RS22995 at 5628.518 kb on - strand, within CA265_RS22995 at 5628.547 kb on + strand, within CA265_RS22995 at 5628.548 kb on - strand, within CA265_RS22995 at 5628.590 kb on - strand, within CA265_RS22995 at 5628.598 kb on - strand, within CA265_RS22995 at 5628.641 kb on - strand, within CA265_RS22995 at 5628.653 kb on + strand, within CA265_RS22995 at 5628.688 kb on - strand, within CA265_RS22995 at 5628.732 kb on - strand, within CA265_RS22995 at 5628.757 kb on + strand, within CA265_RS22995 at 5628.757 kb on + strand, within CA265_RS22995 at 5628.786 kb on + strand, within CA265_RS22995 at 5628.802 kb on + strand, within CA265_RS22995 at 5628.840 kb on + strand, within CA265_RS22995 at 5628.843 kb on + strand, within CA265_RS22995 at 5628.843 kb on + strand, within CA265_RS22995 at 5628.844 kb on - strand, within CA265_RS22995 at 5628.855 kb on - strand, within CA265_RS22995 at 5628.855 kb on - strand, within CA265_RS22995 at 5628.883 kb on + strand, within CA265_RS22995 at 5628.985 kb on + strand, within CA265_RS22995 at 5628.993 kb on + strand, within CA265_RS22995 at 5628.994 kb on - strand, within CA265_RS22995 at 5629.025 kb on - strand, within CA265_RS22995 at 5629.059 kb on + strand, within CA265_RS22995 at 5629.062 kb on + strand, within CA265_RS22995
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 5,626,098 - CA265_RS22985 0.77 -0.0 5,626,101 + CA265_RS22985 0.77 +0.3 5,626,129 - CA265_RS22985 0.78 -1.4 5,626,137 + CA265_RS22985 0.78 +0.6 5,626,170 + CA265_RS22985 0.79 -0.0 5,626,192 + CA265_RS22985 0.80 +0.2 5,626,192 + CA265_RS22985 0.80 -1.8 5,626,193 - CA265_RS22985 0.80 -2.7 5,626,264 + CA265_RS22985 0.82 -0.2 5,626,394 - CA265_RS22985 0.87 -0.6 5,626,397 + CA265_RS22985 0.87 +0.3 5,626,397 + CA265_RS22985 0.87 +1.0 5,626,532 + -0.9 5,626,587 - +0.7 5,626,594 - +1.1 5,626,600 + -0.0 5,626,712 + +0.2 5,626,734 - -1.6 5,626,744 - +1.7 5,626,753 - +0.5 5,626,758 + +0.1 5,626,769 - +0.8 5,626,800 + -0.6 5,626,801 - +0.4 5,626,824 + -0.2 5,626,829 + +1.2 5,626,840 + +1.1 5,626,840 + -0.4 5,626,909 + +0.4 5,626,972 - +0.5 5,626,973 + +0.4 5,626,988 + +0.4 5,627,005 + +0.1 5,627,012 - +0.0 5,627,052 - +0.3 5,627,135 + -1.6 5,627,138 - +0.2 5,627,209 - CA265_RS22990 0.15 +0.5 5,627,253 - CA265_RS22990 0.20 -0.1 5,627,263 - CA265_RS22990 0.21 +0.5 5,627,350 - CA265_RS22990 0.29 +0.6 5,627,357 + CA265_RS22990 0.30 -0.2 5,627,357 + CA265_RS22990 0.30 -1.3 5,627,359 + CA265_RS22990 0.30 -1.4 5,627,437 - CA265_RS22990 0.38 +1.1 5,627,447 + CA265_RS22990 0.39 +1.0 5,627,447 + CA265_RS22990 0.39 -0.5 5,627,447 + CA265_RS22990 0.39 +1.6 5,627,448 - CA265_RS22990 0.39 +0.3 5,627,448 - CA265_RS22990 0.39 +1.5 5,627,518 + CA265_RS22990 0.46 +2.0 5,627,542 - CA265_RS22990 0.48 +0.6 5,627,615 - CA265_RS22990 0.55 +1.3 5,627,615 - CA265_RS22990 0.55 +0.6 5,627,623 - CA265_RS22990 0.56 +0.7 5,627,624 + CA265_RS22990 0.56 -0.2 5,627,625 - CA265_RS22990 0.56 -1.1 5,627,625 - CA265_RS22990 0.56 -1.6 5,627,626 + CA265_RS22990 0.57 +0.6 5,627,626 + CA265_RS22990 0.57 +1.2 5,627,750 - CA265_RS22990 0.69 +1.1 5,627,750 - CA265_RS22990 0.69 +0.1 5,627,917 + CA265_RS22990 0.85 +0.3 5,627,918 - CA265_RS22990 0.85 +0.4 5,627,932 - CA265_RS22990 0.87 +0.5 5,627,945 + CA265_RS22990 0.88 +0.5 5,627,945 + CA265_RS22990 0.88 -0.2 5,627,952 - CA265_RS22990 0.89 +0.4 5,627,959 - CA265_RS22990 0.90 +0.4 5,627,982 + +2.6 5,628,013 + +0.1 5,628,022 - +0.7 5,628,079 - +0.2 5,628,122 + +0.3 5,628,123 - +0.6 5,628,126 - +0.7 5,628,126 - +0.7 5,628,182 + -0.5 5,628,200 + -0.6 5,628,225 - -0.5 5,628,285 + +1.5 5,628,285 + +0.7 5,628,315 + +0.0 5,628,350 - -0.6 5,628,350 - +0.4 5,628,426 + CA265_RS22995 0.10 +0.6 5,628,511 + CA265_RS22995 0.13 -0.3 5,628,518 - CA265_RS22995 0.13 -0.5 5,628,547 + CA265_RS22995 0.14 +0.6 5,628,548 - CA265_RS22995 0.14 -0.7 5,628,590 - CA265_RS22995 0.15 +0.5 5,628,598 - CA265_RS22995 0.15 +0.3 5,628,641 - CA265_RS22995 0.16 -0.5 5,628,653 + CA265_RS22995 0.17 +0.7 5,628,688 - CA265_RS22995 0.18 +0.4 5,628,732 - CA265_RS22995 0.19 +1.3 5,628,757 + CA265_RS22995 0.20 +0.3 5,628,757 + CA265_RS22995 0.20 +0.0 5,628,786 + CA265_RS22995 0.21 +0.3 5,628,802 + CA265_RS22995 0.21 -0.5 5,628,840 + CA265_RS22995 0.22 +0.9 5,628,843 + CA265_RS22995 0.22 +0.7 5,628,843 + CA265_RS22995 0.22 +0.7 5,628,844 - CA265_RS22995 0.22 -0.4 5,628,855 - CA265_RS22995 0.22 +0.8 5,628,855 - CA265_RS22995 0.22 +0.2 5,628,883 + CA265_RS22995 0.23 +0.2 5,628,985 + CA265_RS22995 0.26 -0.1 5,628,993 + CA265_RS22995 0.26 -0.3 5,628,994 - CA265_RS22995 0.26 -0.4 5,629,025 - CA265_RS22995 0.27 -0.8 5,629,059 + CA265_RS22995 0.28 -0.4 5,629,062 + CA265_RS22995 0.28 -0.1
Or see this region's nucleotide sequence