Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22220 and CA265_RS22225 are separated by 105 nucleotides CA265_RS22225 and CA265_RS22230 are separated by 1 nucleotides CA265_RS22230 and CA265_RS22235 are separated by 12 nucleotides
CA265_RS22220: CA265_RS22220 - hypothetical protein, at 5,460,213 to 5,460,608
_RS22220
CA265_RS22225: CA265_RS22225 - hypothetical protein, at 5,460,714 to 5,461,265
_RS22225
CA265_RS22230: CA265_RS22230 - hypothetical protein, at 5,461,267 to 5,461,854
_RS22230
CA265_RS22235: CA265_RS22235 - type IV secretion protein Rhs, at 5,461,867 to 5,463,666
_RS22235
Position (kb) 5461
5462 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5460.463 kb on + strand, within CA265_RS22220 at 5460.481 kb on - strand, within CA265_RS22220 at 5460.631 kb on - strand at 5460.638 kb on + strand at 5460.639 kb on - strand at 5460.694 kb on - strand at 5460.694 kb on - strand at 5460.724 kb on + strand at 5460.777 kb on + strand, within CA265_RS22225 at 5460.777 kb on + strand, within CA265_RS22225 at 5460.777 kb on + strand, within CA265_RS22225 at 5460.777 kb on + strand, within CA265_RS22225 at 5460.778 kb on - strand, within CA265_RS22225 at 5460.779 kb on + strand, within CA265_RS22225 at 5460.788 kb on - strand, within CA265_RS22225 at 5460.857 kb on + strand, within CA265_RS22225 at 5460.897 kb on - strand, within CA265_RS22225 at 5460.986 kb on + strand, within CA265_RS22225 at 5461.086 kb on + strand, within CA265_RS22225 at 5461.087 kb on - strand, within CA265_RS22225 at 5461.224 kb on - strand at 5461.224 kb on - strand at 5461.224 kb on - strand at 5461.232 kb on + strand at 5461.233 kb on - strand at 5461.323 kb on + strand at 5461.369 kb on + strand, within CA265_RS22230 at 5461.374 kb on + strand, within CA265_RS22230 at 5461.375 kb on - strand, within CA265_RS22230 at 5461.375 kb on - strand, within CA265_RS22230 at 5461.380 kb on + strand, within CA265_RS22230 at 5461.425 kb on + strand, within CA265_RS22230 at 5461.426 kb on - strand, within CA265_RS22230 at 5461.426 kb on - strand, within CA265_RS22230 at 5461.428 kb on + strand, within CA265_RS22230 at 5461.429 kb on - strand, within CA265_RS22230 at 5461.540 kb on - strand, within CA265_RS22230 at 5461.555 kb on - strand, within CA265_RS22230 at 5461.620 kb on + strand, within CA265_RS22230 at 5461.620 kb on + strand, within CA265_RS22230 at 5461.738 kb on - strand, within CA265_RS22230 at 5461.738 kb on - strand, within CA265_RS22230 at 5461.788 kb on + strand, within CA265_RS22230 at 5461.789 kb on - strand, within CA265_RS22230 at 5461.789 kb on - strand, within CA265_RS22230 at 5461.894 kb on - strand at 5461.894 kb on - strand at 5461.898 kb on + strand at 5461.899 kb on - strand at 5461.933 kb on - strand at 5461.933 kb on - strand at 5461.937 kb on + strand at 5461.951 kb on - strand at 5461.964 kb on - strand at 5461.981 kb on - strand at 5462.164 kb on + strand, within CA265_RS22235 at 5462.279 kb on + strand, within CA265_RS22235 at 5462.279 kb on + strand, within CA265_RS22235 at 5462.307 kb on + strand, within CA265_RS22235 at 5462.307 kb on + strand, within CA265_RS22235 at 5462.314 kb on + strand, within CA265_RS22235 at 5462.315 kb on - strand at 5462.315 kb on - strand, within CA265_RS22235 at 5462.315 kb on - strand, within CA265_RS22235 at 5462.386 kb on + strand, within CA265_RS22235 at 5462.477 kb on - strand, within CA265_RS22235 at 5462.477 kb on - strand, within CA265_RS22235 at 5462.518 kb on + strand, within CA265_RS22235 at 5462.597 kb on + strand at 5462.598 kb on - strand, within CA265_RS22235 at 5462.617 kb on + strand, within CA265_RS22235 at 5462.622 kb on + strand, within CA265_RS22235 at 5462.654 kb on + strand, within CA265_RS22235 at 5462.655 kb on - strand, within CA265_RS22235 at 5462.707 kb on - strand, within CA265_RS22235 at 5462.707 kb on - strand, within CA265_RS22235 at 5462.726 kb on + strand, within CA265_RS22235 at 5462.784 kb on + strand, within CA265_RS22235 at 5462.808 kb on + strand, within CA265_RS22235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 5,460,463 + CA265_RS22220 0.63 +0.4 5,460,481 - CA265_RS22220 0.68 -1.9 5,460,631 - +0.5 5,460,638 + -1.1 5,460,639 - -0.2 5,460,694 - -0.6 5,460,694 - -2.2 5,460,724 + -0.1 5,460,777 + CA265_RS22225 0.11 -0.5 5,460,777 + CA265_RS22225 0.11 -1.4 5,460,777 + CA265_RS22225 0.11 +0.0 5,460,777 + CA265_RS22225 0.11 -0.3 5,460,778 - CA265_RS22225 0.12 +0.0 5,460,779 + CA265_RS22225 0.12 -0.0 5,460,788 - CA265_RS22225 0.13 +0.1 5,460,857 + CA265_RS22225 0.26 -2.8 5,460,897 - CA265_RS22225 0.33 +0.0 5,460,986 + CA265_RS22225 0.49 +2.0 5,461,086 + CA265_RS22225 0.67 -0.3 5,461,087 - CA265_RS22225 0.68 -0.1 5,461,224 - -0.3 5,461,224 - -0.0 5,461,224 - +0.4 5,461,232 + -0.6 5,461,233 - -1.0 5,461,323 + +0.1 5,461,369 + CA265_RS22230 0.17 -0.0 5,461,374 + CA265_RS22230 0.18 -1.0 5,461,375 - CA265_RS22230 0.18 -0.6 5,461,375 - CA265_RS22230 0.18 -0.4 5,461,380 + CA265_RS22230 0.19 -0.5 5,461,425 + CA265_RS22230 0.27 -0.6 5,461,426 - CA265_RS22230 0.27 +1.3 5,461,426 - CA265_RS22230 0.27 +0.8 5,461,428 + CA265_RS22230 0.27 +0.1 5,461,429 - CA265_RS22230 0.28 -1.3 5,461,540 - CA265_RS22230 0.46 -1.7 5,461,555 - CA265_RS22230 0.49 -1.0 5,461,620 + CA265_RS22230 0.60 -1.2 5,461,620 + CA265_RS22230 0.60 -0.5 5,461,738 - CA265_RS22230 0.80 +0.2 5,461,738 - CA265_RS22230 0.80 +0.4 5,461,788 + CA265_RS22230 0.89 -0.3 5,461,789 - CA265_RS22230 0.89 -0.3 5,461,789 - CA265_RS22230 0.89 +0.2 5,461,894 - -0.7 5,461,894 - -0.1 5,461,898 + -1.0 5,461,899 - -0.5 5,461,933 - -0.6 5,461,933 - +0.3 5,461,937 + -0.3 5,461,951 - +1.2 5,461,964 - +0.7 5,461,981 - -0.3 5,462,164 + CA265_RS22235 0.17 +0.2 5,462,279 + CA265_RS22235 0.23 -2.8 5,462,279 + CA265_RS22235 0.23 -1.6 5,462,307 + CA265_RS22235 0.24 +2.6 5,462,307 + CA265_RS22235 0.24 -0.5 5,462,314 + CA265_RS22235 0.25 -0.1 5,462,315 - -0.6 5,462,315 - CA265_RS22235 0.25 -0.6 5,462,315 - CA265_RS22235 0.25 +0.7 5,462,386 + CA265_RS22235 0.29 +0.2 5,462,477 - CA265_RS22235 0.34 +0.3 5,462,477 - CA265_RS22235 0.34 +0.5 5,462,518 + CA265_RS22235 0.36 -0.4 5,462,597 + +1.0 5,462,598 - CA265_RS22235 0.41 -1.4 5,462,617 + CA265_RS22235 0.42 -2.2 5,462,622 + CA265_RS22235 0.42 -0.9 5,462,654 + CA265_RS22235 0.44 -0.7 5,462,655 - CA265_RS22235 0.44 -0.3 5,462,707 - CA265_RS22235 0.47 -0.5 5,462,707 - CA265_RS22235 0.47 +1.0 5,462,726 + CA265_RS22235 0.48 +0.7 5,462,784 + CA265_RS22235 0.51 +0.3 5,462,808 + CA265_RS22235 0.52 +0.5
Or see this region's nucleotide sequence