Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18485 and CA265_RS18490 are separated by 30 nucleotides CA265_RS18490 and CA265_RS18495 overlap by 1 nucleotides CA265_RS18495 and CA265_RS18500 are separated by 40 nucleotides CA265_RS18500 and CA265_RS18505 are separated by 120 nucleotides
CA265_RS18485: CA265_RS18485 - arginosuccinate lyase (EC 4.3.2.1) (from data) , at 4,599,274 to 4,600,605
_RS18485
CA265_RS18490: CA265_RS18490 - hypothetical protein, at 4,600,636 to 4,600,968
_RS18490
CA265_RS18495: CA265_RS18495 - hypothetical protein, at 4,600,968 to 4,601,189
_RS18495
CA265_RS18500: CA265_RS18500 - N-succinylcitrulline desuccinylase (EC 3.5.1.-) (from data) , at 4,601,230 to 4,602,297
_RS18500
CA265_RS18505: CA265_RS18505 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 4,602,418 to 4,603,227
_RS18505
Position (kb)
4601
4602
4603 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4600.346 kb on - strand, within CA265_RS18485 at 4600.409 kb on + strand, within CA265_RS18485 at 4600.409 kb on + strand, within CA265_RS18485 at 4600.504 kb on + strand at 4600.505 kb on - strand at 4600.577 kb on - strand at 4600.618 kb on - strand at 4600.688 kb on - strand, within CA265_RS18490 at 4600.693 kb on + strand, within CA265_RS18490 at 4600.702 kb on + strand, within CA265_RS18490 at 4600.723 kb on + strand, within CA265_RS18490 at 4600.734 kb on + strand, within CA265_RS18490 at 4600.785 kb on + strand, within CA265_RS18490 at 4600.824 kb on - strand, within CA265_RS18490 at 4600.928 kb on - strand, within CA265_RS18490 at 4600.929 kb on + strand, within CA265_RS18490 at 4600.929 kb on + strand, within CA265_RS18490 at 4600.930 kb on - strand, within CA265_RS18490 at 4600.945 kb on - strand at 4600.980 kb on - strand at 4600.982 kb on + strand at 4601.036 kb on + strand, within CA265_RS18495 at 4601.060 kb on + strand, within CA265_RS18495 at 4601.206 kb on - strand at 4601.211 kb on - strand at 4601.284 kb on + strand at 4601.293 kb on - strand at 4601.366 kb on - strand, within CA265_RS18500 at 4601.399 kb on + strand, within CA265_RS18500 at 4601.487 kb on + strand, within CA265_RS18500 at 4601.525 kb on - strand, within CA265_RS18500 at 4601.558 kb on - strand, within CA265_RS18500 at 4601.558 kb on - strand, within CA265_RS18500 at 4601.619 kb on + strand, within CA265_RS18500 at 4601.706 kb on + strand, within CA265_RS18500 at 4601.706 kb on + strand, within CA265_RS18500 at 4601.708 kb on + strand, within CA265_RS18500 at 4601.708 kb on + strand, within CA265_RS18500 at 4601.801 kb on - strand, within CA265_RS18500 at 4601.948 kb on + strand, within CA265_RS18500 at 4601.977 kb on + strand, within CA265_RS18500 at 4601.978 kb on - strand, within CA265_RS18500 at 4601.998 kb on - strand, within CA265_RS18500 at 4601.998 kb on - strand, within CA265_RS18500 at 4602.041 kb on + strand, within CA265_RS18500 at 4602.072 kb on + strand, within CA265_RS18500 at 4602.134 kb on + strand, within CA265_RS18500 at 4602.134 kb on + strand, within CA265_RS18500 at 4602.228 kb on + strand at 4602.297 kb on + strand at 4602.297 kb on + strand at 4602.297 kb on + strand at 4602.298 kb on - strand at 4602.301 kb on - strand at 4602.317 kb on + strand at 4602.318 kb on - strand at 4602.351 kb on + strand at 4602.430 kb on - strand at 4602.430 kb on - strand at 4602.528 kb on + strand, within CA265_RS18505 at 4602.529 kb on - strand, within CA265_RS18505 at 4602.529 kb on - strand, within CA265_RS18505 at 4602.547 kb on + strand, within CA265_RS18505 at 4602.548 kb on - strand, within CA265_RS18505 at 4602.552 kb on + strand, within CA265_RS18505 at 4602.552 kb on + strand, within CA265_RS18505 at 4602.553 kb on - strand, within CA265_RS18505 at 4602.553 kb on - strand, within CA265_RS18505 at 4602.556 kb on + strand, within CA265_RS18505 at 4602.557 kb on - strand, within CA265_RS18505 at 4602.586 kb on + strand, within CA265_RS18505 at 4602.586 kb on + strand, within CA265_RS18505 at 4602.587 kb on - strand, within CA265_RS18505 at 4602.661 kb on + strand, within CA265_RS18505 at 4602.684 kb on + strand, within CA265_RS18505 at 4602.754 kb on + strand, within CA265_RS18505 at 4603.006 kb on + strand, within CA265_RS18505 at 4603.007 kb on - strand, within CA265_RS18505 at 4603.083 kb on - strand, within CA265_RS18505 at 4603.102 kb on - strand, within CA265_RS18505 at 4603.102 kb on - strand, within CA265_RS18505 at 4603.102 kb on - strand, within CA265_RS18505 at 4603.104 kb on - strand, within CA265_RS18505 at 4603.134 kb on + strand, within CA265_RS18505 at 4603.155 kb on - strand at 4603.155 kb on - strand at 4603.186 kb on + strand at 4603.210 kb on + strand at 4603.211 kb on - strand at 4603.267 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 4,600,346 - CA265_RS18485 0.80 +1.0 4,600,409 + CA265_RS18485 0.85 +1.1 4,600,409 + CA265_RS18485 0.85 +2.0 4,600,504 + +0.2 4,600,505 - -0.0 4,600,577 - +1.3 4,600,618 - -1.5 4,600,688 - CA265_RS18490 0.16 +0.9 4,600,693 + CA265_RS18490 0.17 +1.0 4,600,702 + CA265_RS18490 0.20 +0.6 4,600,723 + CA265_RS18490 0.26 -1.1 4,600,734 + CA265_RS18490 0.29 +0.3 4,600,785 + CA265_RS18490 0.45 +2.0 4,600,824 - CA265_RS18490 0.56 -0.5 4,600,928 - CA265_RS18490 0.88 -0.9 4,600,929 + CA265_RS18490 0.88 +1.4 4,600,929 + CA265_RS18490 0.88 +0.3 4,600,930 - CA265_RS18490 0.88 +0.4 4,600,945 - +0.2 4,600,980 - +0.5 4,600,982 + +0.9 4,601,036 + CA265_RS18495 0.31 +0.5 4,601,060 + CA265_RS18495 0.41 +1.6 4,601,206 - -1.0 4,601,211 - -0.9 4,601,284 + -0.5 4,601,293 - +0.8 4,601,366 - CA265_RS18500 0.13 -1.2 4,601,399 + CA265_RS18500 0.16 +1.7 4,601,487 + CA265_RS18500 0.24 +1.0 4,601,525 - CA265_RS18500 0.28 -0.3 4,601,558 - CA265_RS18500 0.31 +0.3 4,601,558 - CA265_RS18500 0.31 +0.3 4,601,619 + CA265_RS18500 0.36 +0.5 4,601,706 + CA265_RS18500 0.45 +0.5 4,601,706 + CA265_RS18500 0.45 +1.9 4,601,708 + CA265_RS18500 0.45 -0.9 4,601,708 + CA265_RS18500 0.45 -1.0 4,601,801 - CA265_RS18500 0.53 +1.3 4,601,948 + CA265_RS18500 0.67 -0.5 4,601,977 + CA265_RS18500 0.70 +0.2 4,601,978 - CA265_RS18500 0.70 +1.1 4,601,998 - CA265_RS18500 0.72 -0.7 4,601,998 - CA265_RS18500 0.72 +0.3 4,602,041 + CA265_RS18500 0.76 +0.5 4,602,072 + CA265_RS18500 0.79 +1.4 4,602,134 + CA265_RS18500 0.85 +1.3 4,602,134 + CA265_RS18500 0.85 +0.3 4,602,228 + +0.3 4,602,297 + +1.0 4,602,297 + -0.1 4,602,297 + +0.0 4,602,298 - -0.9 4,602,301 - -0.9 4,602,317 + +0.7 4,602,318 - -2.4 4,602,351 + +0.0 4,602,430 - +1.1 4,602,430 - +0.7 4,602,528 + CA265_RS18505 0.14 -0.4 4,602,529 - CA265_RS18505 0.14 -0.7 4,602,529 - CA265_RS18505 0.14 -1.5 4,602,547 + CA265_RS18505 0.16 +0.2 4,602,548 - CA265_RS18505 0.16 +0.1 4,602,552 + CA265_RS18505 0.17 +1.7 4,602,552 + CA265_RS18505 0.17 +0.0 4,602,553 - CA265_RS18505 0.17 +0.0 4,602,553 - CA265_RS18505 0.17 -0.7 4,602,556 + CA265_RS18505 0.17 +0.4 4,602,557 - CA265_RS18505 0.17 -0.7 4,602,586 + CA265_RS18505 0.21 -0.1 4,602,586 + CA265_RS18505 0.21 +0.1 4,602,587 - CA265_RS18505 0.21 -1.8 4,602,661 + CA265_RS18505 0.30 -0.3 4,602,684 + CA265_RS18505 0.33 +2.3 4,602,754 + CA265_RS18505 0.41 -0.4 4,603,006 + CA265_RS18505 0.73 +0.3 4,603,007 - CA265_RS18505 0.73 +0.5 4,603,083 - CA265_RS18505 0.82 -0.8 4,603,102 - CA265_RS18505 0.84 +0.3 4,603,102 - CA265_RS18505 0.84 +0.1 4,603,102 - CA265_RS18505 0.84 -1.2 4,603,104 - CA265_RS18505 0.85 -0.4 4,603,134 + CA265_RS18505 0.88 -1.7 4,603,155 - +0.7 4,603,155 - -0.4 4,603,186 + +0.1 4,603,210 + -0.7 4,603,211 - -1.2 4,603,267 + +0.2
Or see this region's nucleotide sequence