Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13215 and CA265_RS13220 are separated by 66 nucleotides CA265_RS13220 and CA265_RS13225 are separated by 274 nucleotides CA265_RS13225 and CA265_RS13230 are separated by 42 nucleotides CA265_RS13230 and CA265_RS13235 overlap by 1 nucleotides
CA265_RS13215: CA265_RS13215 - hypothetical protein, at 3,286,514 to 3,287,173
_RS13215
CA265_RS13220: CA265_RS13220 - hypothetical protein, at 3,287,240 to 3,287,845
_RS13220
CA265_RS13225: CA265_RS13225 - exonuclease, at 3,288,120 to 3,289,061
_RS13225
CA265_RS13230: CA265_RS13230 - dephospho-CoA kinase, at 3,289,104 to 3,289,721
_RS13230
CA265_RS13235: CA265_RS13235 - hypothetical protein, at 3,289,721 to 3,290,578
_RS13235
Position (kb)
3288
3289
3290 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3287.201 kb on - strand at 3287.390 kb on - strand, within CA265_RS13220 at 3287.456 kb on + strand, within CA265_RS13220 at 3287.496 kb on + strand, within CA265_RS13220 at 3287.523 kb on - strand, within CA265_RS13220 at 3287.523 kb on - strand, within CA265_RS13220 at 3287.581 kb on - strand, within CA265_RS13220 at 3287.581 kb on - strand, within CA265_RS13220 at 3287.711 kb on + strand, within CA265_RS13220 at 3287.711 kb on + strand, within CA265_RS13220 at 3287.767 kb on - strand, within CA265_RS13220 at 3287.767 kb on - strand, within CA265_RS13220 at 3287.769 kb on + strand, within CA265_RS13220 at 3287.770 kb on - strand, within CA265_RS13220 at 3287.770 kb on - strand, within CA265_RS13220 at 3287.797 kb on - strand at 3287.830 kb on + strand at 3287.831 kb on - strand at 3287.889 kb on - strand at 3287.955 kb on + strand at 3287.981 kb on + strand at 3288.044 kb on + strand at 3288.047 kb on + strand at 3288.078 kb on - strand at 3288.093 kb on + strand at 3288.096 kb on - strand at 3288.173 kb on + strand at 3288.174 kb on - strand at 3288.187 kb on - strand at 3288.326 kb on + strand, within CA265_RS13225 at 3288.339 kb on + strand, within CA265_RS13225 at 3288.340 kb on + strand, within CA265_RS13225 at 3288.442 kb on + strand, within CA265_RS13225 at 3288.442 kb on + strand, within CA265_RS13225 at 3288.443 kb on - strand, within CA265_RS13225 at 3288.451 kb on + strand, within CA265_RS13225 at 3288.473 kb on + strand, within CA265_RS13225 at 3288.473 kb on + strand, within CA265_RS13225 at 3288.489 kb on + strand, within CA265_RS13225 at 3288.547 kb on - strand, within CA265_RS13225 at 3288.597 kb on + strand, within CA265_RS13225 at 3288.606 kb on + strand, within CA265_RS13225 at 3288.644 kb on + strand, within CA265_RS13225 at 3288.645 kb on - strand, within CA265_RS13225 at 3288.645 kb on - strand, within CA265_RS13225 at 3288.660 kb on + strand, within CA265_RS13225 at 3288.661 kb on - strand, within CA265_RS13225 at 3288.714 kb on - strand, within CA265_RS13225 at 3288.738 kb on + strand, within CA265_RS13225 at 3288.746 kb on + strand, within CA265_RS13225 at 3288.749 kb on - strand, within CA265_RS13225 at 3288.756 kb on + strand, within CA265_RS13225 at 3288.779 kb on + strand, within CA265_RS13225 at 3288.780 kb on - strand, within CA265_RS13225 at 3288.846 kb on - strand, within CA265_RS13225 at 3288.908 kb on + strand, within CA265_RS13225 at 3288.914 kb on - strand, within CA265_RS13225 at 3289.044 kb on + strand at 3289.089 kb on + strand at 3289.090 kb on - strand at 3289.122 kb on - strand at 3289.885 kb on + strand, within CA265_RS13235 at 3289.890 kb on - strand, within CA265_RS13235 at 3289.934 kb on - strand, within CA265_RS13235 at 3289.934 kb on - strand, within CA265_RS13235 at 3289.954 kb on + strand, within CA265_RS13235 at 3290.056 kb on + strand, within CA265_RS13235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 3,287,201 - -0.5 3,287,390 - CA265_RS13220 0.25 +0.5 3,287,456 + CA265_RS13220 0.36 -0.6 3,287,496 + CA265_RS13220 0.42 +0.5 3,287,523 - CA265_RS13220 0.47 +0.2 3,287,523 - CA265_RS13220 0.47 -1.6 3,287,581 - CA265_RS13220 0.56 -1.1 3,287,581 - CA265_RS13220 0.56 +0.4 3,287,711 + CA265_RS13220 0.78 +0.0 3,287,711 + CA265_RS13220 0.78 +0.6 3,287,767 - CA265_RS13220 0.87 +0.7 3,287,767 - CA265_RS13220 0.87 +1.4 3,287,769 + CA265_RS13220 0.87 -1.0 3,287,770 - CA265_RS13220 0.87 +0.3 3,287,770 - CA265_RS13220 0.87 -0.6 3,287,797 - -0.2 3,287,830 + +0.4 3,287,831 - -0.1 3,287,889 - +0.1 3,287,955 + +0.2 3,287,981 + -3.1 3,288,044 + -0.6 3,288,047 + +0.9 3,288,078 - -0.2 3,288,093 + +0.4 3,288,096 - +0.6 3,288,173 + +1.1 3,288,174 - +0.6 3,288,187 - +2.1 3,288,326 + CA265_RS13225 0.22 +0.6 3,288,339 + CA265_RS13225 0.23 +0.3 3,288,340 + CA265_RS13225 0.23 -0.4 3,288,442 + CA265_RS13225 0.34 +0.5 3,288,442 + CA265_RS13225 0.34 -0.5 3,288,443 - CA265_RS13225 0.34 +1.0 3,288,451 + CA265_RS13225 0.35 +0.8 3,288,473 + CA265_RS13225 0.37 +1.4 3,288,473 + CA265_RS13225 0.37 +1.1 3,288,489 + CA265_RS13225 0.39 +0.7 3,288,547 - CA265_RS13225 0.45 +1.1 3,288,597 + CA265_RS13225 0.51 +0.6 3,288,606 + CA265_RS13225 0.52 +0.9 3,288,644 + CA265_RS13225 0.56 +1.6 3,288,645 - CA265_RS13225 0.56 +0.1 3,288,645 - CA265_RS13225 0.56 -0.2 3,288,660 + CA265_RS13225 0.57 +0.1 3,288,661 - CA265_RS13225 0.57 +0.9 3,288,714 - CA265_RS13225 0.63 +0.3 3,288,738 + CA265_RS13225 0.66 +1.3 3,288,746 + CA265_RS13225 0.66 +0.9 3,288,749 - CA265_RS13225 0.67 +1.0 3,288,756 + CA265_RS13225 0.68 +1.0 3,288,779 + CA265_RS13225 0.70 +0.9 3,288,780 - CA265_RS13225 0.70 +1.1 3,288,846 - CA265_RS13225 0.77 +0.6 3,288,908 + CA265_RS13225 0.84 +1.0 3,288,914 - CA265_RS13225 0.84 +0.8 3,289,044 + +0.6 3,289,089 + +0.7 3,289,090 - -0.5 3,289,122 - +1.4 3,289,885 + CA265_RS13235 0.19 -0.4 3,289,890 - CA265_RS13235 0.20 +0.0 3,289,934 - CA265_RS13235 0.25 +0.0 3,289,934 - CA265_RS13235 0.25 +0.2 3,289,954 + CA265_RS13235 0.27 -2.1 3,290,056 + CA265_RS13235 0.39 -0.3
Or see this region's nucleotide sequence