Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08030 and CA265_RS08035 are separated by 122 nucleotides CA265_RS08035 and CA265_RS08040 are separated by 133 nucleotides
CA265_RS08030: CA265_RS08030 - NADPH:quinone reductase, at 2,000,220 to 2,001,233
_RS08030
CA265_RS08035: CA265_RS08035 - AraC family transcriptional regulator, at 2,001,356 to 2,002,195
_RS08035
CA265_RS08040: CA265_RS08040 - Cro/Cl family transcriptional regulator, at 2,002,329 to 2,003,243
_RS08040
Position (kb)
2001
2002
2003 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2000.372 kb on + strand, within CA265_RS08030 at 2000.404 kb on + strand, within CA265_RS08030 at 2000.420 kb on - strand, within CA265_RS08030 at 2000.420 kb on - strand, within CA265_RS08030 at 2000.492 kb on + strand, within CA265_RS08030 at 2000.545 kb on + strand, within CA265_RS08030 at 2000.574 kb on - strand, within CA265_RS08030 at 2000.631 kb on + strand, within CA265_RS08030 at 2000.693 kb on + strand, within CA265_RS08030 at 2000.698 kb on - strand, within CA265_RS08030 at 2000.698 kb on - strand, within CA265_RS08030 at 2000.746 kb on - strand, within CA265_RS08030 at 2000.765 kb on + strand, within CA265_RS08030 at 2000.766 kb on - strand, within CA265_RS08030 at 2000.877 kb on - strand, within CA265_RS08030 at 2000.893 kb on - strand, within CA265_RS08030 at 2000.936 kb on + strand, within CA265_RS08030 at 2000.937 kb on - strand, within CA265_RS08030 at 2000.965 kb on + strand, within CA265_RS08030 at 2001.015 kb on + strand, within CA265_RS08030 at 2001.066 kb on - strand, within CA265_RS08030 at 2001.072 kb on - strand, within CA265_RS08030 at 2001.072 kb on - strand, within CA265_RS08030 at 2001.077 kb on + strand, within CA265_RS08030 at 2001.251 kb on - strand at 2001.253 kb on - strand at 2001.271 kb on - strand at 2001.347 kb on + strand at 2001.453 kb on + strand, within CA265_RS08035 at 2001.455 kb on + strand, within CA265_RS08035 at 2001.509 kb on + strand, within CA265_RS08035 at 2001.603 kb on + strand, within CA265_RS08035 at 2001.604 kb on - strand, within CA265_RS08035 at 2001.605 kb on + strand, within CA265_RS08035 at 2001.654 kb on - strand, within CA265_RS08035 at 2001.689 kb on - strand, within CA265_RS08035 at 2001.754 kb on + strand, within CA265_RS08035 at 2001.756 kb on + strand, within CA265_RS08035 at 2001.757 kb on - strand, within CA265_RS08035 at 2001.770 kb on + strand, within CA265_RS08035 at 2001.773 kb on - strand, within CA265_RS08035 at 2001.783 kb on + strand, within CA265_RS08035 at 2001.799 kb on - strand, within CA265_RS08035 at 2001.888 kb on + strand, within CA265_RS08035 at 2001.910 kb on + strand, within CA265_RS08035 at 2001.961 kb on + strand, within CA265_RS08035 at 2002.022 kb on + strand, within CA265_RS08035 at 2002.023 kb on - strand, within CA265_RS08035 at 2002.023 kb on - strand, within CA265_RS08035 at 2002.025 kb on - strand, within CA265_RS08035 at 2002.049 kb on - strand, within CA265_RS08035 at 2002.198 kb on + strand at 2002.198 kb on + strand at 2002.227 kb on + strand at 2002.227 kb on + strand at 2002.227 kb on + strand at 2002.228 kb on - strand at 2002.241 kb on - strand at 2002.329 kb on - strand at 2002.329 kb on - strand at 2002.329 kb on - strand at 2002.350 kb on - strand at 2002.411 kb on + strand at 2002.411 kb on + strand at 2002.417 kb on + strand at 2002.469 kb on + strand, within CA265_RS08040 at 2002.588 kb on + strand, within CA265_RS08040 at 2002.699 kb on + strand, within CA265_RS08040 at 2002.856 kb on + strand, within CA265_RS08040 at 2002.990 kb on - strand, within CA265_RS08040 at 2003.009 kb on + strand, within CA265_RS08040 at 2003.010 kb on - strand, within CA265_RS08040 at 2003.010 kb on - strand, within CA265_RS08040 at 2003.010 kb on - strand, within CA265_RS08040 at 2003.070 kb on - strand, within CA265_RS08040 at 2003.130 kb on - strand, within CA265_RS08040 at 2003.191 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 2,000,372 + CA265_RS08030 0.15 -0.3 2,000,404 + CA265_RS08030 0.18 -0.1 2,000,420 - CA265_RS08030 0.20 -0.4 2,000,420 - CA265_RS08030 0.20 -0.0 2,000,492 + CA265_RS08030 0.27 +0.6 2,000,545 + CA265_RS08030 0.32 +0.2 2,000,574 - CA265_RS08030 0.35 -0.1 2,000,631 + CA265_RS08030 0.41 +0.6 2,000,693 + CA265_RS08030 0.47 +0.2 2,000,698 - CA265_RS08030 0.47 -0.6 2,000,698 - CA265_RS08030 0.47 +0.0 2,000,746 - CA265_RS08030 0.52 +0.1 2,000,765 + CA265_RS08030 0.54 -0.2 2,000,766 - CA265_RS08030 0.54 -0.3 2,000,877 - CA265_RS08030 0.65 +0.2 2,000,893 - CA265_RS08030 0.66 +1.8 2,000,936 + CA265_RS08030 0.71 +1.8 2,000,937 - CA265_RS08030 0.71 -0.0 2,000,965 + CA265_RS08030 0.73 -1.6 2,001,015 + CA265_RS08030 0.78 +0.3 2,001,066 - CA265_RS08030 0.83 -1.4 2,001,072 - CA265_RS08030 0.84 +0.6 2,001,072 - CA265_RS08030 0.84 +0.4 2,001,077 + CA265_RS08030 0.85 +0.5 2,001,251 - -0.4 2,001,253 - -0.7 2,001,271 - +0.0 2,001,347 + -0.5 2,001,453 + CA265_RS08035 0.12 +0.2 2,001,455 + CA265_RS08035 0.12 -0.3 2,001,509 + CA265_RS08035 0.18 -1.4 2,001,603 + CA265_RS08035 0.29 -1.8 2,001,604 - CA265_RS08035 0.30 -0.4 2,001,605 + CA265_RS08035 0.30 -0.2 2,001,654 - CA265_RS08035 0.35 +0.1 2,001,689 - CA265_RS08035 0.40 -0.6 2,001,754 + CA265_RS08035 0.47 -1.3 2,001,756 + CA265_RS08035 0.48 +0.4 2,001,757 - CA265_RS08035 0.48 +0.6 2,001,770 + CA265_RS08035 0.49 -0.3 2,001,773 - CA265_RS08035 0.50 -1.1 2,001,783 + CA265_RS08035 0.51 -2.2 2,001,799 - CA265_RS08035 0.53 -0.1 2,001,888 + CA265_RS08035 0.63 -2.0 2,001,910 + CA265_RS08035 0.66 -0.9 2,001,961 + CA265_RS08035 0.72 +0.3 2,002,022 + CA265_RS08035 0.79 +1.5 2,002,023 - CA265_RS08035 0.79 +0.2 2,002,023 - CA265_RS08035 0.79 +0.4 2,002,025 - CA265_RS08035 0.80 -1.8 2,002,049 - CA265_RS08035 0.82 -0.2 2,002,198 + +0.3 2,002,198 + -0.2 2,002,227 + -0.3 2,002,227 + -0.2 2,002,227 + -0.4 2,002,228 - -0.0 2,002,241 - -0.4 2,002,329 - +0.1 2,002,329 - +1.2 2,002,329 - +1.1 2,002,350 - +0.3 2,002,411 + +0.2 2,002,411 + +1.2 2,002,417 + +0.6 2,002,469 + CA265_RS08040 0.15 -0.2 2,002,588 + CA265_RS08040 0.28 -1.2 2,002,699 + CA265_RS08040 0.40 +0.1 2,002,856 + CA265_RS08040 0.58 +0.8 2,002,990 - CA265_RS08040 0.72 +0.0 2,003,009 + CA265_RS08040 0.74 -0.1 2,003,010 - CA265_RS08040 0.74 -0.3 2,003,010 - CA265_RS08040 0.74 +0.0 2,003,010 - CA265_RS08040 0.74 -0.8 2,003,070 - CA265_RS08040 0.81 +1.0 2,003,130 - CA265_RS08040 0.88 -0.7 2,003,191 - -0.2
Or see this region's nucleotide sequence