Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07445 and CA265_RS07450 are separated by 72 nucleotides CA265_RS07450 and CA265_RS07455 overlap by 4 nucleotides CA265_RS07455 and CA265_RS07460 are separated by 42 nucleotides CA265_RS07460 and CA265_RS07465 are separated by 78 nucleotides
CA265_RS07445: CA265_RS07445 - MarR family transcriptional regulator, at 1,881,716 to 1,882,669
_RS07445
CA265_RS07450: CA265_RS07450 - 3-deoxy-D-manno-octulosonate cytidylyltransferase, at 1,882,742 to 1,883,530
_RS07450
CA265_RS07455: CA265_RS07455 - hypothetical protein, at 1,883,527 to 1,883,730
_RS07455
CA265_RS07460: CA265_RS07460 - deoxynucleoside kinase, at 1,883,773 to 1,884,387
_RS07460
CA265_RS07465: CA265_RS07465 - tryptophan--tRNA ligase, at 1,884,466 to 1,885,464
_RS07465
Position (kb) 1883
1884 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 1882.555 kb on + strand, within CA265_RS07445 at 1882.555 kb on + strand, within CA265_RS07445 at 1882.593 kb on + strand at 1882.664 kb on - strand at 1882.708 kb on - strand at 1882.733 kb on + strand at 1882.734 kb on - strand at 1882.744 kb on - strand at 1882.771 kb on + strand at 1882.776 kb on - strand at 1882.785 kb on - strand at 1882.843 kb on - strand, within CA265_RS07450 at 1882.952 kb on - strand, within CA265_RS07450 at 1882.988 kb on + strand, within CA265_RS07450 at 1882.989 kb on - strand, within CA265_RS07450 at 1883.011 kb on + strand, within CA265_RS07450 at 1883.077 kb on + strand, within CA265_RS07450 at 1883.091 kb on - strand, within CA265_RS07450 at 1883.207 kb on + strand, within CA265_RS07450 at 1883.207 kb on + strand, within CA265_RS07450 at 1883.208 kb on - strand, within CA265_RS07450 at 1883.296 kb on + strand, within CA265_RS07450 at 1883.358 kb on - strand, within CA265_RS07450 at 1883.425 kb on + strand, within CA265_RS07450 at 1883.425 kb on + strand, within CA265_RS07450 at 1883.425 kb on + strand, within CA265_RS07450 at 1883.425 kb on + strand, within CA265_RS07450 at 1883.426 kb on - strand, within CA265_RS07450 at 1883.426 kb on - strand, within CA265_RS07450 at 1883.456 kb on - strand at 1883.530 kb on + strand at 1883.531 kb on - strand at 1883.586 kb on + strand, within CA265_RS07455 at 1883.588 kb on + strand, within CA265_RS07455 at 1883.589 kb on - strand, within CA265_RS07455 at 1883.735 kb on - strand at 1883.742 kb on + strand at 1883.742 kb on + strand at 1883.743 kb on - strand at 1883.747 kb on - strand at 1883.747 kb on - strand at 1883.775 kb on - strand at 1883.800 kb on + strand at 1883.802 kb on + strand at 1883.802 kb on + strand at 1883.848 kb on + strand, within CA265_RS07460 at 1883.853 kb on + strand, within CA265_RS07460 at 1883.983 kb on - strand, within CA265_RS07460 at 1883.994 kb on + strand, within CA265_RS07460 at 1883.995 kb on - strand, within CA265_RS07460 at 1883.998 kb on + strand, within CA265_RS07460 at 1884.049 kb on - strand, within CA265_RS07460 at 1884.052 kb on - strand, within CA265_RS07460 at 1884.052 kb on - strand, within CA265_RS07460 at 1884.075 kb on + strand, within CA265_RS07460 at 1884.098 kb on - strand, within CA265_RS07460 at 1884.106 kb on - strand, within CA265_RS07460 at 1884.130 kb on - strand, within CA265_RS07460 at 1884.169 kb on - strand, within CA265_RS07460 at 1884.363 kb on + strand at 1884.364 kb on - strand at 1884.461 kb on - strand at 1884.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 1,882,555 + CA265_RS07445 0.88 +0.0 1,882,555 + CA265_RS07445 0.88 +0.3 1,882,593 + +0.4 1,882,664 - -0.0 1,882,708 - +0.9 1,882,733 + +0.0 1,882,734 - +0.1 1,882,744 - -0.0 1,882,771 + -1.0 1,882,776 - -0.3 1,882,785 - -0.7 1,882,843 - CA265_RS07450 0.13 -4.4 1,882,952 - CA265_RS07450 0.27 -2.5 1,882,988 + CA265_RS07450 0.31 -3.7 1,882,989 - CA265_RS07450 0.31 -2.3 1,883,011 + CA265_RS07450 0.34 -0.3 1,883,077 + CA265_RS07450 0.42 -2.9 1,883,091 - CA265_RS07450 0.44 -4.8 1,883,207 + CA265_RS07450 0.59 -1.7 1,883,207 + CA265_RS07450 0.59 -3.6 1,883,208 - CA265_RS07450 0.59 -3.7 1,883,296 + CA265_RS07450 0.70 -2.1 1,883,358 - CA265_RS07450 0.78 -2.5 1,883,425 + CA265_RS07450 0.87 -0.3 1,883,425 + CA265_RS07450 0.87 -4.5 1,883,425 + CA265_RS07450 0.87 -3.4 1,883,425 + CA265_RS07450 0.87 -4.7 1,883,426 - CA265_RS07450 0.87 -4.3 1,883,426 - CA265_RS07450 0.87 -4.2 1,883,456 - -3.5 1,883,530 + -1.6 1,883,531 - -1.1 1,883,586 + CA265_RS07455 0.29 -2.3 1,883,588 + CA265_RS07455 0.30 -3.0 1,883,589 - CA265_RS07455 0.30 +0.1 1,883,735 - -0.4 1,883,742 + -1.8 1,883,742 + -1.0 1,883,743 - -0.6 1,883,747 - +0.1 1,883,747 - -0.4 1,883,775 - -0.1 1,883,800 + -1.8 1,883,802 + -2.1 1,883,802 + -0.5 1,883,848 + CA265_RS07460 0.12 -0.7 1,883,853 + CA265_RS07460 0.13 -4.5 1,883,983 - CA265_RS07460 0.34 -1.1 1,883,994 + CA265_RS07460 0.36 -2.7 1,883,995 - CA265_RS07460 0.36 -0.3 1,883,998 + CA265_RS07460 0.37 -2.6 1,884,049 - CA265_RS07460 0.45 -0.6 1,884,052 - CA265_RS07460 0.45 -2.6 1,884,052 - CA265_RS07460 0.45 -0.1 1,884,075 + CA265_RS07460 0.49 -0.9 1,884,098 - CA265_RS07460 0.53 -0.9 1,884,106 - CA265_RS07460 0.54 -0.2 1,884,130 - CA265_RS07460 0.58 -3.1 1,884,169 - CA265_RS07460 0.64 -0.1 1,884,363 + -1.9 1,884,364 - -3.2 1,884,461 - +2.1 1,884,461 - -1.7
Or see this region's nucleotide sequence