Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07250 and CA265_RS07255 are separated by 171 nucleotides CA265_RS07255 and CA265_RS07260 overlap by 4 nucleotides
CA265_RS07250: CA265_RS07250 - hypothetical protein, at 1,816,692 to 1,817,651
_RS07250
CA265_RS07255: CA265_RS07255 - aspartyl beta-hydroxylase, at 1,817,823 to 1,818,512
_RS07255
CA265_RS07260: CA265_RS07260 - hypothetical protein, at 1,818,509 to 1,819,465
_RS07260
Position (kb)
1817
1818
1819 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1816.916 kb on + strand, within CA265_RS07250 at 1816.956 kb on + strand, within CA265_RS07250 at 1816.972 kb on + strand, within CA265_RS07250 at 1816.972 kb on + strand, within CA265_RS07250 at 1817.085 kb on - strand, within CA265_RS07250 at 1817.118 kb on - strand at 1817.118 kb on - strand, within CA265_RS07250 at 1817.188 kb on - strand, within CA265_RS07250 at 1817.344 kb on - strand, within CA265_RS07250 at 1817.378 kb on + strand, within CA265_RS07250 at 1817.472 kb on - strand, within CA265_RS07250 at 1817.486 kb on + strand, within CA265_RS07250 at 1817.529 kb on - strand, within CA265_RS07250 at 1817.680 kb on - strand at 1817.686 kb on + strand at 1817.687 kb on - strand at 1817.795 kb on - strand at 1817.832 kb on + strand at 1817.833 kb on - strand at 1817.854 kb on - strand at 1817.893 kb on + strand, within CA265_RS07255 at 1817.987 kb on + strand, within CA265_RS07255 at 1817.988 kb on - strand, within CA265_RS07255 at 1818.001 kb on - strand, within CA265_RS07255 at 1818.008 kb on - strand, within CA265_RS07255 at 1818.053 kb on + strand, within CA265_RS07255 at 1818.054 kb on - strand, within CA265_RS07255 at 1818.057 kb on + strand, within CA265_RS07255 at 1818.179 kb on - strand, within CA265_RS07255 at 1818.179 kb on - strand, within CA265_RS07255 at 1818.182 kb on + strand, within CA265_RS07255 at 1818.182 kb on + strand, within CA265_RS07255 at 1818.183 kb on - strand, within CA265_RS07255 at 1818.183 kb on - strand, within CA265_RS07255 at 1818.262 kb on - strand, within CA265_RS07255 at 1818.288 kb on - strand, within CA265_RS07255 at 1818.328 kb on + strand, within CA265_RS07255 at 1818.328 kb on + strand, within CA265_RS07255 at 1818.337 kb on + strand, within CA265_RS07255 at 1818.379 kb on - strand, within CA265_RS07255 at 1818.381 kb on - strand, within CA265_RS07255 at 1818.381 kb on - strand, within CA265_RS07255 at 1818.430 kb on + strand, within CA265_RS07255 at 1818.666 kb on - strand, within CA265_RS07260 at 1818.666 kb on - strand, within CA265_RS07260 at 1818.724 kb on - strand, within CA265_RS07260 at 1818.772 kb on - strand, within CA265_RS07260 at 1818.805 kb on + strand, within CA265_RS07260 at 1818.941 kb on + strand, within CA265_RS07260 at 1818.942 kb on - strand, within CA265_RS07260 at 1818.996 kb on + strand, within CA265_RS07260 at 1819.007 kb on + strand, within CA265_RS07260 at 1819.083 kb on + strand, within CA265_RS07260 at 1819.083 kb on + strand, within CA265_RS07260 at 1819.083 kb on + strand, within CA265_RS07260 at 1819.084 kb on - strand, within CA265_RS07260 at 1819.084 kb on - strand, within CA265_RS07260 at 1819.131 kb on + strand, within CA265_RS07260 at 1819.188 kb on - strand, within CA265_RS07260 at 1819.188 kb on - strand, within CA265_RS07260 at 1819.241 kb on + strand, within CA265_RS07260 at 1819.290 kb on + strand, within CA265_RS07260 at 1819.400 kb on + strand at 1819.400 kb on + strand at 1819.444 kb on - strand at 1819.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 1,816,916 + CA265_RS07250 0.23 -0.0 1,816,956 + CA265_RS07250 0.28 -0.6 1,816,972 + CA265_RS07250 0.29 -0.6 1,816,972 + CA265_RS07250 0.29 +0.1 1,817,085 - CA265_RS07250 0.41 +0.1 1,817,118 - -0.3 1,817,118 - CA265_RS07250 0.44 -0.9 1,817,188 - CA265_RS07250 0.52 -0.2 1,817,344 - CA265_RS07250 0.68 -0.1 1,817,378 + CA265_RS07250 0.71 +0.4 1,817,472 - CA265_RS07250 0.81 +0.4 1,817,486 + CA265_RS07250 0.83 -0.4 1,817,529 - CA265_RS07250 0.87 -0.2 1,817,680 - +0.9 1,817,686 + -2.2 1,817,687 - -1.5 1,817,795 - -1.5 1,817,832 + -3.0 1,817,833 - -0.9 1,817,854 - +0.6 1,817,893 + CA265_RS07255 0.10 +0.5 1,817,987 + CA265_RS07255 0.24 +0.5 1,817,988 - CA265_RS07255 0.24 +0.4 1,818,001 - CA265_RS07255 0.26 -0.8 1,818,008 - CA265_RS07255 0.27 +0.2 1,818,053 + CA265_RS07255 0.33 +0.3 1,818,054 - CA265_RS07255 0.33 -0.0 1,818,057 + CA265_RS07255 0.34 +0.5 1,818,179 - CA265_RS07255 0.52 +1.0 1,818,179 - CA265_RS07255 0.52 +0.2 1,818,182 + CA265_RS07255 0.52 +1.1 1,818,182 + CA265_RS07255 0.52 +0.1 1,818,183 - CA265_RS07255 0.52 +0.3 1,818,183 - CA265_RS07255 0.52 -0.1 1,818,262 - CA265_RS07255 0.64 +0.4 1,818,288 - CA265_RS07255 0.67 +0.1 1,818,328 + CA265_RS07255 0.73 -0.7 1,818,328 + CA265_RS07255 0.73 +0.7 1,818,337 + CA265_RS07255 0.74 +1.1 1,818,379 - CA265_RS07255 0.81 -0.5 1,818,381 - CA265_RS07255 0.81 +0.6 1,818,381 - CA265_RS07255 0.81 +0.1 1,818,430 + CA265_RS07255 0.88 +1.3 1,818,666 - CA265_RS07260 0.16 +0.4 1,818,666 - CA265_RS07260 0.16 +0.0 1,818,724 - CA265_RS07260 0.22 +0.6 1,818,772 - CA265_RS07260 0.27 +0.2 1,818,805 + CA265_RS07260 0.31 -0.3 1,818,941 + CA265_RS07260 0.45 +0.4 1,818,942 - CA265_RS07260 0.45 +0.3 1,818,996 + CA265_RS07260 0.51 -0.2 1,819,007 + CA265_RS07260 0.52 +0.3 1,819,083 + CA265_RS07260 0.60 -0.0 1,819,083 + CA265_RS07260 0.60 +0.1 1,819,083 + CA265_RS07260 0.60 +0.3 1,819,084 - CA265_RS07260 0.60 -0.4 1,819,084 - CA265_RS07260 0.60 -0.1 1,819,131 + CA265_RS07260 0.65 -0.3 1,819,188 - CA265_RS07260 0.71 -0.6 1,819,188 - CA265_RS07260 0.71 -0.2 1,819,241 + CA265_RS07260 0.76 -1.2 1,819,290 + CA265_RS07260 0.82 -0.1 1,819,400 + +0.2 1,819,400 + +0.1 1,819,444 - +1.0 1,819,444 - +1.1
Or see this region's nucleotide sequence