Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06045 and CA265_RS06050 are separated by 118 nucleotides CA265_RS06050 and CA265_RS06055 are separated by 140 nucleotides
CA265_RS06045: CA265_RS06045 - arylsulfatase, at 1,482,580 to 1,484,235
_RS06045
CA265_RS06050: CA265_RS06050 - hypothetical protein, at 1,484,354 to 1,488,397
_RS06050
CA265_RS06055: CA265_RS06055 - neuraminidase, at 1,488,538 to 1,489,863
_RS06055
Position (kb) 1484
1485
1486
1487
1488
1489 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1483.379 kb on + strand, within CA265_RS06045 at 1483.390 kb on - strand, within CA265_RS06045 at 1483.390 kb on - strand, within CA265_RS06045 at 1483.477 kb on + strand, within CA265_RS06045 at 1483.478 kb on - strand, within CA265_RS06045 at 1483.498 kb on - strand, within CA265_RS06045 at 1483.551 kb on - strand, within CA265_RS06045 at 1483.555 kb on - strand, within CA265_RS06045 at 1483.557 kb on + strand, within CA265_RS06045 at 1483.641 kb on + strand, within CA265_RS06045 at 1483.641 kb on + strand, within CA265_RS06045 at 1483.654 kb on - strand, within CA265_RS06045 at 1483.663 kb on + strand, within CA265_RS06045 at 1483.664 kb on - strand, within CA265_RS06045 at 1483.738 kb on - strand, within CA265_RS06045 at 1483.740 kb on + strand, within CA265_RS06045 at 1483.874 kb on - strand, within CA265_RS06045 at 1483.925 kb on + strand, within CA265_RS06045 at 1483.926 kb on - strand, within CA265_RS06045 at 1483.992 kb on + strand, within CA265_RS06045 at 1484.023 kb on + strand, within CA265_RS06045 at 1484.023 kb on + strand, within CA265_RS06045 at 1484.024 kb on - strand, within CA265_RS06045 at 1484.024 kb on - strand, within CA265_RS06045 at 1484.137 kb on + strand at 1484.137 kb on + strand at 1484.188 kb on - strand at 1484.192 kb on + strand at 1484.233 kb on + strand at 1484.264 kb on + strand at 1484.308 kb on - strand at 1484.308 kb on - strand at 1484.431 kb on + strand at 1484.437 kb on + strand at 1484.451 kb on - strand at 1484.451 kb on - strand at 1484.480 kb on - strand at 1484.488 kb on - strand at 1484.498 kb on + strand at 1484.498 kb on + strand at 1484.499 kb on - strand at 1484.499 kb on - strand at 1484.561 kb on + strand at 1484.570 kb on - strand at 1484.661 kb on + strand at 1484.666 kb on - strand at 1484.687 kb on + strand at 1484.687 kb on + strand at 1484.688 kb on - strand at 1484.724 kb on - strand at 1484.752 kb on - strand at 1484.778 kb on + strand, within CA265_RS06050 at 1484.778 kb on + strand, within CA265_RS06050 at 1484.779 kb on - strand, within CA265_RS06050 at 1484.802 kb on + strand, within CA265_RS06050 at 1484.803 kb on - strand, within CA265_RS06050 at 1484.902 kb on - strand, within CA265_RS06050 at 1484.903 kb on + strand, within CA265_RS06050 at 1484.956 kb on + strand, within CA265_RS06050 at 1484.957 kb on - strand, within CA265_RS06050 at 1484.957 kb on - strand, within CA265_RS06050 at 1484.978 kb on - strand, within CA265_RS06050 at 1485.022 kb on + strand, within CA265_RS06050 at 1485.034 kb on + strand, within CA265_RS06050 at 1485.035 kb on - strand, within CA265_RS06050 at 1485.035 kb on - strand at 1485.134 kb on - strand, within CA265_RS06050 at 1485.134 kb on - strand, within CA265_RS06050 at 1485.150 kb on + strand, within CA265_RS06050 at 1485.203 kb on + strand, within CA265_RS06050 at 1485.230 kb on + strand, within CA265_RS06050 at 1485.340 kb on + strand at 1485.409 kb on + strand, within CA265_RS06050 at 1485.409 kb on + strand, within CA265_RS06050 at 1485.660 kb on - strand, within CA265_RS06050 at 1485.662 kb on - strand, within CA265_RS06050 at 1485.668 kb on + strand, within CA265_RS06050 at 1485.668 kb on + strand, within CA265_RS06050 at 1485.670 kb on + strand, within CA265_RS06050 at 1485.671 kb on - strand, within CA265_RS06050 at 1485.673 kb on + strand, within CA265_RS06050 at 1485.698 kb on - strand, within CA265_RS06050 at 1485.816 kb on + strand, within CA265_RS06050 at 1485.817 kb on - strand, within CA265_RS06050 at 1485.913 kb on + strand, within CA265_RS06050 at 1485.918 kb on - strand, within CA265_RS06050 at 1485.973 kb on - strand, within CA265_RS06050 at 1486.037 kb on + strand, within CA265_RS06050 at 1486.037 kb on + strand, within CA265_RS06050 at 1486.100 kb on - strand, within CA265_RS06050 at 1486.104 kb on + strand, within CA265_RS06050 at 1486.118 kb on + strand, within CA265_RS06050 at 1486.118 kb on + strand, within CA265_RS06050 at 1486.119 kb on - strand, within CA265_RS06050 at 1486.145 kb on - strand, within CA265_RS06050 at 1486.154 kb on - strand, within CA265_RS06050 at 1486.174 kb on + strand, within CA265_RS06050 at 1486.282 kb on + strand, within CA265_RS06050 at 1486.288 kb on + strand, within CA265_RS06050 at 1486.305 kb on + strand, within CA265_RS06050 at 1486.376 kb on - strand, within CA265_RS06050 at 1486.376 kb on - strand, within CA265_RS06050 at 1486.376 kb on - strand, within CA265_RS06050 at 1486.402 kb on - strand, within CA265_RS06050 at 1486.414 kb on + strand, within CA265_RS06050 at 1486.449 kb on + strand, within CA265_RS06050 at 1486.450 kb on - strand, within CA265_RS06050 at 1486.484 kb on + strand, within CA265_RS06050 at 1486.521 kb on + strand, within CA265_RS06050 at 1486.522 kb on - strand, within CA265_RS06050 at 1486.530 kb on + strand, within CA265_RS06050 at 1486.534 kb on + strand, within CA265_RS06050 at 1486.535 kb on - strand, within CA265_RS06050 at 1486.535 kb on - strand, within CA265_RS06050 at 1486.536 kb on - strand, within CA265_RS06050 at 1486.544 kb on + strand, within CA265_RS06050 at 1486.568 kb on + strand, within CA265_RS06050 at 1486.579 kb on + strand, within CA265_RS06050 at 1486.661 kb on - strand, within CA265_RS06050 at 1486.675 kb on + strand, within CA265_RS06050 at 1486.676 kb on - strand, within CA265_RS06050 at 1486.676 kb on - strand, within CA265_RS06050 at 1486.676 kb on - strand, within CA265_RS06050 at 1486.683 kb on - strand, within CA265_RS06050 at 1486.687 kb on + strand, within CA265_RS06050 at 1486.704 kb on + strand, within CA265_RS06050 at 1486.774 kb on + strand, within CA265_RS06050 at 1486.829 kb on - strand, within CA265_RS06050 at 1486.974 kb on + strand, within CA265_RS06050 at 1486.975 kb on - strand, within CA265_RS06050 at 1486.981 kb on - strand, within CA265_RS06050 at 1486.985 kb on - strand, within CA265_RS06050 at 1487.008 kb on + strand, within CA265_RS06050 at 1487.132 kb on - strand, within CA265_RS06050 at 1487.132 kb on - strand, within CA265_RS06050 at 1487.135 kb on + strand, within CA265_RS06050 at 1487.170 kb on + strand, within CA265_RS06050 at 1487.191 kb on + strand, within CA265_RS06050 at 1487.191 kb on + strand, within CA265_RS06050 at 1487.224 kb on + strand, within CA265_RS06050 at 1487.231 kb on - strand, within CA265_RS06050 at 1487.324 kb on - strand, within CA265_RS06050 at 1487.324 kb on - strand, within CA265_RS06050 at 1487.329 kb on - strand, within CA265_RS06050 at 1487.329 kb on - strand at 1487.392 kb on - strand, within CA265_RS06050 at 1487.425 kb on + strand, within CA265_RS06050 at 1487.466 kb on + strand, within CA265_RS06050 at 1487.520 kb on + strand, within CA265_RS06050 at 1487.649 kb on - strand, within CA265_RS06050 at 1487.722 kb on + strand, within CA265_RS06050 at 1487.733 kb on + strand, within CA265_RS06050 at 1487.757 kb on + strand, within CA265_RS06050 at 1487.758 kb on - strand, within CA265_RS06050 at 1487.758 kb on - strand, within CA265_RS06050 at 1487.791 kb on + strand, within CA265_RS06050 at 1487.929 kb on - strand, within CA265_RS06050 at 1487.929 kb on - strand, within CA265_RS06050 at 1487.954 kb on - strand, within CA265_RS06050 at 1487.954 kb on - strand, within CA265_RS06050 at 1487.987 kb on + strand, within CA265_RS06050 at 1488.092 kb on - strand at 1488.180 kb on + strand at 1488.181 kb on - strand at 1488.200 kb on - strand at 1488.236 kb on - strand at 1488.248 kb on - strand at 1488.277 kb on - strand at 1488.283 kb on + strand at 1488.287 kb on - strand at 1488.371 kb on + strand at 1488.376 kb on + strand at 1488.379 kb on + strand at 1488.392 kb on - strand at 1488.512 kb on + strand at 1488.563 kb on + strand at 1488.564 kb on - strand at 1488.565 kb on + strand at 1488.577 kb on - strand at 1488.662 kb on + strand at 1488.725 kb on - strand, within CA265_RS06055 at 1488.725 kb on - strand, within CA265_RS06055 at 1488.725 kb on - strand, within CA265_RS06055 at 1488.788 kb on + strand, within CA265_RS06055 at 1488.789 kb on - strand, within CA265_RS06055 at 1488.838 kb on - strand, within CA265_RS06055 at 1488.843 kb on + strand, within CA265_RS06055 at 1488.844 kb on - strand, within CA265_RS06055 at 1488.849 kb on - strand, within CA265_RS06055 at 1488.889 kb on - strand, within CA265_RS06055 at 1488.985 kb on + strand, within CA265_RS06055 at 1489.106 kb on + strand, within CA265_RS06055 at 1489.148 kb on - strand, within CA265_RS06055 at 1489.163 kb on + strand, within CA265_RS06055 at 1489.217 kb on + strand, within CA265_RS06055 at 1489.217 kb on + strand, within CA265_RS06055 at 1489.236 kb on - strand, within CA265_RS06055 at 1489.236 kb on - strand, within CA265_RS06055 at 1489.245 kb on + strand, within CA265_RS06055 at 1489.281 kb on + strand, within CA265_RS06055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 1,483,379 + CA265_RS06045 0.48 -0.2 1,483,390 - CA265_RS06045 0.49 -0.2 1,483,390 - CA265_RS06045 0.49 +0.3 1,483,477 + CA265_RS06045 0.54 -0.5 1,483,478 - CA265_RS06045 0.54 -0.1 1,483,498 - CA265_RS06045 0.55 +1.2 1,483,551 - CA265_RS06045 0.59 +0.5 1,483,555 - CA265_RS06045 0.59 +0.3 1,483,557 + CA265_RS06045 0.59 -1.5 1,483,641 + CA265_RS06045 0.64 -0.6 1,483,641 + CA265_RS06045 0.64 -0.0 1,483,654 - CA265_RS06045 0.65 +0.2 1,483,663 + CA265_RS06045 0.65 +0.1 1,483,664 - CA265_RS06045 0.65 +1.3 1,483,738 - CA265_RS06045 0.70 +0.4 1,483,740 + CA265_RS06045 0.70 +0.1 1,483,874 - CA265_RS06045 0.78 -0.4 1,483,925 + CA265_RS06045 0.81 -0.2 1,483,926 - CA265_RS06045 0.81 -0.7 1,483,992 + CA265_RS06045 0.85 -0.5 1,484,023 + CA265_RS06045 0.87 +0.4 1,484,023 + CA265_RS06045 0.87 -1.0 1,484,024 - CA265_RS06045 0.87 -0.2 1,484,024 - CA265_RS06045 0.87 +0.4 1,484,137 + -0.0 1,484,137 + -0.4 1,484,188 - -0.0 1,484,192 + +1.3 1,484,233 + -0.1 1,484,264 + -0.7 1,484,308 - +0.2 1,484,308 - -0.2 1,484,431 + -0.8 1,484,437 + +0.5 1,484,451 - -0.4 1,484,451 - -0.0 1,484,480 - -0.4 1,484,488 - +1.0 1,484,498 + -0.4 1,484,498 + -0.4 1,484,499 - +0.3 1,484,499 - -0.1 1,484,561 + +0.0 1,484,570 - +0.8 1,484,661 + -0.5 1,484,666 - +0.1 1,484,687 + +0.8 1,484,687 + -1.3 1,484,688 - -0.1 1,484,724 - -0.5 1,484,752 - -0.1 1,484,778 + CA265_RS06050 0.10 -1.0 1,484,778 + CA265_RS06050 0.10 -0.9 1,484,779 - CA265_RS06050 0.11 +0.2 1,484,802 + CA265_RS06050 0.11 -0.2 1,484,803 - CA265_RS06050 0.11 +0.4 1,484,902 - CA265_RS06050 0.14 +0.2 1,484,903 + CA265_RS06050 0.14 -0.9 1,484,956 + CA265_RS06050 0.15 +1.3 1,484,957 - CA265_RS06050 0.15 -0.6 1,484,957 - CA265_RS06050 0.15 +0.0 1,484,978 - CA265_RS06050 0.15 -0.7 1,485,022 + CA265_RS06050 0.17 +0.2 1,485,034 + CA265_RS06050 0.17 -0.1 1,485,035 - CA265_RS06050 0.17 -0.1 1,485,035 - +2.3 1,485,134 - CA265_RS06050 0.19 +0.1 1,485,134 - CA265_RS06050 0.19 -1.0 1,485,150 + CA265_RS06050 0.20 -0.3 1,485,203 + CA265_RS06050 0.21 -0.1 1,485,230 + CA265_RS06050 0.22 +0.1 1,485,340 + +2.3 1,485,409 + CA265_RS06050 0.26 +0.1 1,485,409 + CA265_RS06050 0.26 +1.1 1,485,660 - CA265_RS06050 0.32 +0.5 1,485,662 - CA265_RS06050 0.32 -0.0 1,485,668 + CA265_RS06050 0.32 -0.4 1,485,668 + CA265_RS06050 0.32 -0.7 1,485,670 + CA265_RS06050 0.33 -0.1 1,485,671 - CA265_RS06050 0.33 +0.2 1,485,673 + CA265_RS06050 0.33 -0.9 1,485,698 - CA265_RS06050 0.33 +0.2 1,485,816 + CA265_RS06050 0.36 -0.6 1,485,817 - CA265_RS06050 0.36 +0.4 1,485,913 + CA265_RS06050 0.39 -0.6 1,485,918 - CA265_RS06050 0.39 +0.2 1,485,973 - CA265_RS06050 0.40 +0.1 1,486,037 + CA265_RS06050 0.42 +0.0 1,486,037 + CA265_RS06050 0.42 +0.8 1,486,100 - CA265_RS06050 0.43 -0.2 1,486,104 + CA265_RS06050 0.43 +0.2 1,486,118 + CA265_RS06050 0.44 +0.4 1,486,118 + CA265_RS06050 0.44 +0.2 1,486,119 - CA265_RS06050 0.44 -0.3 1,486,145 - CA265_RS06050 0.44 +0.5 1,486,154 - CA265_RS06050 0.45 +0.2 1,486,174 + CA265_RS06050 0.45 +0.5 1,486,282 + CA265_RS06050 0.48 +0.4 1,486,288 + CA265_RS06050 0.48 +0.8 1,486,305 + CA265_RS06050 0.48 +0.4 1,486,376 - CA265_RS06050 0.50 +0.0 1,486,376 - CA265_RS06050 0.50 +0.5 1,486,376 - CA265_RS06050 0.50 +0.9 1,486,402 - CA265_RS06050 0.51 -0.9 1,486,414 + CA265_RS06050 0.51 +0.1 1,486,449 + CA265_RS06050 0.52 -0.8 1,486,450 - CA265_RS06050 0.52 -0.6 1,486,484 + CA265_RS06050 0.53 -0.5 1,486,521 + CA265_RS06050 0.54 +0.3 1,486,522 - CA265_RS06050 0.54 -0.1 1,486,530 + CA265_RS06050 0.54 +0.3 1,486,534 + CA265_RS06050 0.54 +0.7 1,486,535 - CA265_RS06050 0.54 -0.4 1,486,535 - CA265_RS06050 0.54 -0.6 1,486,536 - CA265_RS06050 0.54 +0.3 1,486,544 + CA265_RS06050 0.54 +0.7 1,486,568 + CA265_RS06050 0.55 -0.3 1,486,579 + CA265_RS06050 0.55 -0.6 1,486,661 - CA265_RS06050 0.57 +0.8 1,486,675 + CA265_RS06050 0.57 -0.4 1,486,676 - CA265_RS06050 0.57 -0.5 1,486,676 - CA265_RS06050 0.57 +0.3 1,486,676 - CA265_RS06050 0.57 +0.2 1,486,683 - CA265_RS06050 0.58 -0.2 1,486,687 + CA265_RS06050 0.58 +0.4 1,486,704 + CA265_RS06050 0.58 +0.4 1,486,774 + CA265_RS06050 0.60 -0.7 1,486,829 - CA265_RS06050 0.61 -0.9 1,486,974 + CA265_RS06050 0.65 -0.1 1,486,975 - CA265_RS06050 0.65 -0.1 1,486,981 - CA265_RS06050 0.65 -0.2 1,486,985 - CA265_RS06050 0.65 -0.3 1,487,008 + CA265_RS06050 0.66 +0.1 1,487,132 - CA265_RS06050 0.69 -1.1 1,487,132 - CA265_RS06050 0.69 -0.3 1,487,135 + CA265_RS06050 0.69 -0.4 1,487,170 + CA265_RS06050 0.70 +1.3 1,487,191 + CA265_RS06050 0.70 -0.8 1,487,191 + CA265_RS06050 0.70 -0.0 1,487,224 + CA265_RS06050 0.71 -0.2 1,487,231 - CA265_RS06050 0.71 +0.2 1,487,324 - CA265_RS06050 0.73 +0.8 1,487,324 - CA265_RS06050 0.73 +0.8 1,487,329 - CA265_RS06050 0.74 -0.8 1,487,329 - -0.7 1,487,392 - CA265_RS06050 0.75 -0.4 1,487,425 + CA265_RS06050 0.76 -0.9 1,487,466 + CA265_RS06050 0.77 +0.3 1,487,520 + CA265_RS06050 0.78 -0.1 1,487,649 - CA265_RS06050 0.81 +2.9 1,487,722 + CA265_RS06050 0.83 +0.5 1,487,733 + CA265_RS06050 0.84 -0.4 1,487,757 + CA265_RS06050 0.84 +0.4 1,487,758 - CA265_RS06050 0.84 +1.3 1,487,758 - CA265_RS06050 0.84 -0.4 1,487,791 + CA265_RS06050 0.85 +0.1 1,487,929 - CA265_RS06050 0.88 -0.1 1,487,929 - CA265_RS06050 0.88 -0.8 1,487,954 - CA265_RS06050 0.89 +0.3 1,487,954 - CA265_RS06050 0.89 +0.5 1,487,987 + CA265_RS06050 0.90 +0.1 1,488,092 - -0.2 1,488,180 + +0.1 1,488,181 - -1.8 1,488,200 - -0.7 1,488,236 - +0.0 1,488,248 - +0.2 1,488,277 - +0.6 1,488,283 + +0.0 1,488,287 - +0.0 1,488,371 + -1.0 1,488,376 + +0.9 1,488,379 + +0.9 1,488,392 - -0.2 1,488,512 + -0.1 1,488,563 + -0.2 1,488,564 - +0.0 1,488,565 + +0.3 1,488,577 - +0.1 1,488,662 + +0.6 1,488,725 - CA265_RS06055 0.14 -0.4 1,488,725 - CA265_RS06055 0.14 -0.9 1,488,725 - CA265_RS06055 0.14 -1.0 1,488,788 + CA265_RS06055 0.19 -1.8 1,488,789 - CA265_RS06055 0.19 +0.1 1,488,838 - CA265_RS06055 0.23 -0.7 1,488,843 + CA265_RS06055 0.23 +0.8 1,488,844 - CA265_RS06055 0.23 +0.8 1,488,849 - CA265_RS06055 0.23 -0.2 1,488,889 - CA265_RS06055 0.26 +0.4 1,488,985 + CA265_RS06055 0.34 -0.1 1,489,106 + CA265_RS06055 0.43 -0.5 1,489,148 - CA265_RS06055 0.46 +0.1 1,489,163 + CA265_RS06055 0.47 +0.2 1,489,217 + CA265_RS06055 0.51 -1.7 1,489,217 + CA265_RS06055 0.51 +1.2 1,489,236 - CA265_RS06055 0.53 -0.1 1,489,236 - CA265_RS06055 0.53 -0.3 1,489,245 + CA265_RS06055 0.53 +1.0 1,489,281 + CA265_RS06055 0.56 +0.5
Or see this region's nucleotide sequence